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Article

Inhibitory effects of Cinnamomum burmannii Blume stem bark extract and trans-cinnamaldehyde on nasopharyngeal carcinoma cells; synergism with cisplatin

  • Authors:
    • Maelinda Daker
    • Voon Yee Lin
    • Gabriel Akyirem Akowuah
    • Mun Fei Yam
    • Mariam Ahmad
  • View Affiliations / Copyright

    Affiliations: Molecular Pathology Unit, Institute for Medical Research, Jalan Pahang, Kuala Lumpur 50588, Malaysia, Faculty of Pharmaceutical Sciences, UCSI University, Kuala Lumpur 56000, Malaysia, Faculty of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Penang 11800, Malaysia
  • Pages: 1701-1709
    |
    Published online on: April 2, 2013
       https://doi.org/10.3892/etm.2013.1041
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Abstract

Nasopharyngeal carcinoma (NPC) is a malignancy that occurs in the epithelium of the nasopharynx. The standard treatment of NPC patients with locoregionally advanced stages is problematic and is often associated with toxicities. Therefore, it is essential to screen for naturally occurring compounds with strong apoptosis-inducing activity and minimal toxicity. This study investigated the effects of the standardized methanol extract of Cinnamomum burmannii Blume stem bark and its main constituent, trans-cinnamaldehyde (TCA), on human NPC cell lines. The content of TCA in C. burmannii methanol extract was standardized to be 13.61% w/w by means of gas chromatography-mass spectrometry (GC-MS). NPC cell proliferation was clearly inhibited within 24 h of treatment, with TCA exhibiting greater activity than the methanol extract. TCA was more active against NPC cells compared with cisplatin. There was a pronounced downregulation of the proliferation markers, Ki67 and proliferating cell nuclear antigen (PCNA) in the TCA-treated cells; while morphological observation indicated the induction of apoptosis. Caspase activation and prominent DNA damage, which are markers of apoptosis induction were detected. TCA demonstrated the ability to scavenge nitric oxide. The simultaneous combination of TCA and cisplatin produced synergistic anti-proliferative effects. Collectively, these data indicate the potential use of TCA for the treatment of NPC.
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Copy and paste a formatted citation
Spandidos Publications style
Daker M, Lin VY, Akowuah GA, Yam MF and Ahmad M: Inhibitory effects of Cinnamomum burmannii Blume stem bark extract and trans-cinnamaldehyde on nasopharyngeal carcinoma cells; synergism with cisplatin. Exp Ther Med 5: 1701-1709, 2013.
APA
Daker, M., Lin, V.Y., Akowuah, G.A., Yam, M.F., & Ahmad, M. (2013). Inhibitory effects of Cinnamomum burmannii Blume stem bark extract and trans-cinnamaldehyde on nasopharyngeal carcinoma cells; synergism with cisplatin. Experimental and Therapeutic Medicine, 5, 1701-1709. https://doi.org/10.3892/etm.2013.1041
MLA
Daker, M., Lin, V. Y., Akowuah, G. A., Yam, M. F., Ahmad, M."Inhibitory effects of Cinnamomum burmannii Blume stem bark extract and trans-cinnamaldehyde on nasopharyngeal carcinoma cells; synergism with cisplatin". Experimental and Therapeutic Medicine 5.6 (2013): 1701-1709.
Chicago
Daker, M., Lin, V. Y., Akowuah, G. A., Yam, M. F., Ahmad, M."Inhibitory effects of Cinnamomum burmannii Blume stem bark extract and trans-cinnamaldehyde on nasopharyngeal carcinoma cells; synergism with cisplatin". Experimental and Therapeutic Medicine 5, no. 6 (2013): 1701-1709. https://doi.org/10.3892/etm.2013.1041
Copy and paste a formatted citation
x
Spandidos Publications style
Daker M, Lin VY, Akowuah GA, Yam MF and Ahmad M: Inhibitory effects of Cinnamomum burmannii Blume stem bark extract and trans-cinnamaldehyde on nasopharyngeal carcinoma cells; synergism with cisplatin. Exp Ther Med 5: 1701-1709, 2013.
APA
Daker, M., Lin, V.Y., Akowuah, G.A., Yam, M.F., & Ahmad, M. (2013). Inhibitory effects of Cinnamomum burmannii Blume stem bark extract and trans-cinnamaldehyde on nasopharyngeal carcinoma cells; synergism with cisplatin. Experimental and Therapeutic Medicine, 5, 1701-1709. https://doi.org/10.3892/etm.2013.1041
MLA
Daker, M., Lin, V. Y., Akowuah, G. A., Yam, M. F., Ahmad, M."Inhibitory effects of Cinnamomum burmannii Blume stem bark extract and trans-cinnamaldehyde on nasopharyngeal carcinoma cells; synergism with cisplatin". Experimental and Therapeutic Medicine 5.6 (2013): 1701-1709.
Chicago
Daker, M., Lin, V. Y., Akowuah, G. A., Yam, M. F., Ahmad, M."Inhibitory effects of Cinnamomum burmannii Blume stem bark extract and trans-cinnamaldehyde on nasopharyngeal carcinoma cells; synergism with cisplatin". Experimental and Therapeutic Medicine 5, no. 6 (2013): 1701-1709. https://doi.org/10.3892/etm.2013.1041
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