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Experimental and Therapeutic Medicine
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Print ISSN: 1792-0981 Online ISSN: 1792-1015
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June 2013 Volume 5 Issue 6

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

Expression and characterization of cecropinXJ, a bioactive antimicrobial peptide from Bombyx mori (Bombycidae, Lepidoptera) in Escherichia coli

  • Authors:
    • Lijie Xia
    • Fuchun Zhang
    • Zhongyuan Liu
    • Ji Ma
    • Jianhua Yang

  • View Affiliations / Copyright

    Affiliations: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, Xinjiang 830046, P.R. China, Department of Pediatrics, Section of Hematology/Oncology, Baylor College of Medicine, Houston, TX 77030, USA
  • Pages: 1745-1751
    |
    Published online on: April 9, 2013
       https://doi.org/10.3892/etm.2013.1056
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Abstract

Insect antimicrobial peptides (AMPs) have a broad antimicrobial spectrum. To aid the characterization of the gene function and further applications, we cloned the gene of cecropinXJ into the prokaryotic expression vector pET32a and expressed cecropinXJ in Escherichia coli BL2l (DE3). Following induction by isopropyl-β-D-thiogalactoside (IPTG), a 25 kDa fusion peptide of cecropinXJ with a tagged thioredoxin (Trx) protein was highly expressed in E. coli. The yield was 10 mg/l culture medium following purification on nickel‑nitrilotriacetic acid (Ni-NTA) metal affinity chromatography matrices. The purified recombinant antibacterial peptide, cecropinXJ, retained a high stability against Staphylococcus aureus over a temperature range from 4 to 100˚C and a pH range from pH 2.0 to 12.0. The minimum inhibitory concentration (MIC) of the fusion protein against S. aureus was 0.4 µM. The recombinant cecropinXJ is also cytotoxic to several types of human cancer cells.

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Copy and paste a formatted citation
Spandidos Publications style
Xia L, Zhang F, Liu Z, Ma J and Yang J: Expression and characterization of cecropinXJ, a bioactive antimicrobial peptide from Bombyx mori (Bombycidae, Lepidoptera) in Escherichia coli. Exp Ther Med 5: 1745-1751, 2013.
APA
Xia, L., Zhang, F., Liu, Z., Ma, J., & Yang, J. (2013). Expression and characterization of cecropinXJ, a bioactive antimicrobial peptide from Bombyx mori (Bombycidae, Lepidoptera) in Escherichia coli. Experimental and Therapeutic Medicine, 5, 1745-1751. https://doi.org/10.3892/etm.2013.1056
MLA
Xia, L., Zhang, F., Liu, Z., Ma, J., Yang, J."Expression and characterization of cecropinXJ, a bioactive antimicrobial peptide from Bombyx mori (Bombycidae, Lepidoptera) in Escherichia coli". Experimental and Therapeutic Medicine 5.6 (2013): 1745-1751.
Chicago
Xia, L., Zhang, F., Liu, Z., Ma, J., Yang, J."Expression and characterization of cecropinXJ, a bioactive antimicrobial peptide from Bombyx mori (Bombycidae, Lepidoptera) in Escherichia coli". Experimental and Therapeutic Medicine 5, no. 6 (2013): 1745-1751. https://doi.org/10.3892/etm.2013.1056
Copy and paste a formatted citation
x
Spandidos Publications style
Xia L, Zhang F, Liu Z, Ma J and Yang J: Expression and characterization of cecropinXJ, a bioactive antimicrobial peptide from Bombyx mori (Bombycidae, Lepidoptera) in Escherichia coli. Exp Ther Med 5: 1745-1751, 2013.
APA
Xia, L., Zhang, F., Liu, Z., Ma, J., & Yang, J. (2013). Expression and characterization of cecropinXJ, a bioactive antimicrobial peptide from Bombyx mori (Bombycidae, Lepidoptera) in Escherichia coli. Experimental and Therapeutic Medicine, 5, 1745-1751. https://doi.org/10.3892/etm.2013.1056
MLA
Xia, L., Zhang, F., Liu, Z., Ma, J., Yang, J."Expression and characterization of cecropinXJ, a bioactive antimicrobial peptide from Bombyx mori (Bombycidae, Lepidoptera) in Escherichia coli". Experimental and Therapeutic Medicine 5.6 (2013): 1745-1751.
Chicago
Xia, L., Zhang, F., Liu, Z., Ma, J., Yang, J."Expression and characterization of cecropinXJ, a bioactive antimicrobial peptide from Bombyx mori (Bombycidae, Lepidoptera) in Escherichia coli". Experimental and Therapeutic Medicine 5, no. 6 (2013): 1745-1751. https://doi.org/10.3892/etm.2013.1056
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