Clinical value of a whole blood interferon‑γ release assay for the diagnosis of Mycobacterium tuberculosis infection during antitubercular treatment

Wang,Haifeng; Li,Zhenyun; Zhang,Qunmei; Lu,Guangjian; Wang,Qingjiang; Zhang,Ligong; Zhang,Chenguang

doi:10.3892/etm.2013.1127

August 2013 Volume 6 Issue 2

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August 2013 Volume 6 Issue 2

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Article

Clinical value of a whole blood interferon‑γ release assay for the diagnosis of Mycobacterium tuberculosis infection during antitubercular treatment

Authors:
- Haifeng Wang
- Zhenyun Li
- Qunmei Zhang
- Guangjian Lu
- Qingjiang Wang
- Ligong Zhang
- Chenguang Zhang
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Affiliations: The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China, Department of Laboratory Medicine, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China
Pages: 455-458
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Published online on: May 21, 2013

https://doi.org/10.3892/etm.2013.1127
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Abstract

The aim of this study was to evaluate the sensitivity and specificity of a whole blood interferon‑γ release assay, the QuantiFERON®‑TB Gold In‑Tube (QFT‑GIT) test, in the diagnosis of Mycobacterium tuberculosis (MTB) infection, and to assess its monitoring role during antitubercular treatment. In total, 20 patients received the QFT test, along with other commonly‑used tests, prior to, and following, 2‑ and 6‑month courses of antitubercular treatment; the results were compared and statistically analyzed. The rate of positive results for tuberculosis (TB) was 95% for the QFT test, which was significantly higher compared with those for the purified protein derivative (PPD; 55%) and the antitubercular antibody tests (15%), as well as the acid‑fast bacilli smear (20%) and cultures for TB (20%; P<0.05 for all). The sensitivity and specificity of the QFT test were 96 and 93.8%, respectively. The positive result rate obtained with the QFT test was significantly higher in the TB group compared with that in the non‑TB group (6.3%; P<0.05). Moreover, the positive result rate obtained with the QFT test was significantly lower in the 6‑month‑treated group compared with that in the 2‑month group (P<0.05). In conclusion, the QFT test is a sensitive and specific method for rapidly diagnosing MTB infection, and has an improved practical clinical value in evaluating antitubercular therapies compared with that of the PPD test.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Clinical value of a whole blood interferon‑γ release assay for the diagnosis of Mycobacterium tuberculosis infection during antitubercular treatment

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