In vitro study on the feasibility of magnetic stent hyperthermia for the treatment of cardiovascular restenosis

Li,Li; Wang,Rui; Shi,Huan‑Huan; Xie,Le; Li,Jing‑Ding‑Sha; Kong,Wei‑Chao; Tang,Jin‑Tian; Ke,Da‑Nian; Zhao,Ling‑Yun

doi:10.3892/etm.2013.1177

August 2013 Volume 6 Issue 2

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

August 2013 Volume 6 Issue 2

Full Size Image

Article

In vitro study on the feasibility of magnetic stent hyperthermia for the treatment of cardiovascular restenosis

Authors:
- Li Li
- Rui Wang
- Huan‑Huan Shi
- Le Xie
- Jing‑Ding‑Sha Li
- Wei‑Chao Kong
- Jin‑Tian Tang
- Da‑Nian Ke
- Ling‑Yun Zhao
View Affiliations / Copyright

Affiliations: Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Institute of Medical Physics and Engineering, Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Department of Engineering Physics, Tsinghua University, Beijing 100084, P.R. China, Beijing MED Zenith Medical Scientific Co. Ltd, Beijing 101312, P.R. China
Pages: 347-354
|
Published online on: June 21, 2013

https://doi.org/10.3892/etm.2013.1177
Expand metrics +

Abstract

Thermal treatment or hyperthermia has received considerable attention in recent years due to its high efficiency, safety and relatively few side-effects. In this study, we investigated whether it was possible to utilize targeted thermal or in‑stent thermal treatments for the treatment of restenosis following percutaneous transluminal coronary angioplasty (PTCA) through magnetic stent hyperthermia (MSH). A 316L stainless steel stent and rabbit vascular smooth muscle cells (VSMCs) were used in the present study, in which the inductive heating characteristics of the stent under alternative magnetic field (AMF) exposure, as well as the effect of MSH on the proliferation, apoptosis, cell cycle and proliferating cell nuclear antigen (PCNA) expression of the rabbit VSMCs, were evaluated. The results demonstrated that 316L stainless steel coronary stents possess ideal inductive heating characteristics under 300 kHz AMF exposure. The heating properties were shown to be affected by the field intensity of the AMF, as well as the orientation the stent axis. MSH had a significant effect on the proliferation and apoptosis of VSMCs, and the effect was temperature‑dependent. While a mild temperature of 43˚C demonstrated negligible effects on the growth of VSMCs, MSH treatment above 47˚C effectively inhibited the VSMC proliferation and induced apoptosis. Furthermore, a 47˚C treatment exhibited a significant and long‑term inhibitory effect on VSMC migration. The results strongly suggested that MSH may be potentially applied in the clinic as an alternative approach for the prevention and treatment of restenosis.

View Figures

View References

1.	Gryn SE and Hackam DG: Lifetime risk prediction in cardiovascular prevention: wave of the future? Can J Cardiol. 29:142–143. 2013.PubMed/NCBI
2.	Riegler J, Liew A, Hynes SO, et al: Superparamagnetic iron oxide nanoparticle targeting of MSCs in vascular injury. Biomaterials. 34:1987–1994. 2013. View Article : Google Scholar : PubMed/NCBI
3.	Grüntzig AR, Senning Å and Siegenthaler WE: Nonoperative dilatation of coronary-artery stenosis - percutaneous transluminal coronary angioplasty. N Engl J Med. 301:61–68. 1979.PubMed/NCBI
4.	Puranik AS, Dawson ER and Peppas NA: Recent advances in drug eluting stents. Int J Pharm. 441:665–679. 2013. View Article : Google Scholar : PubMed/NCBI
5.	Wei Y, Ji Y, Xiao LL, Lin QK, Xu JP, Ren KF and Ji J: Surface engineering of cardiovascular stent with endothelial cell selectivity for in vivo re-endothelialisation. Biomaterials. 34:2588–2599. 2013. View Article : Google Scholar : PubMed/NCBI
6.	Chen X and Fujise K: Restenosis: Emerging molecular targets: Going beyond drug-eluting stents. Drug Discov Today: Dis Mech. 2:1–9. 2005. View Article : Google Scholar
7.	Latib A, Mussardo M, Ielasi A, et al: Long-term outcomes after the percutaneous treatment of drug-eluting stent restenosis. JACC Cardiovasc Interv. 4:155–164. 2011. View Article : Google Scholar : PubMed/NCBI
8.	Kishore R and Losordo DW: Gene therapy for restenosis: Biological solution to a biological problem. J Mol Cell Cardiol. 42:461–468. 2007. View Article : Google Scholar : PubMed/NCBI
9.	Movahed MR: Brachytherapy with gamma radiation of a coronary artery for instent restenosis may induce the regression of in-stent restenosis of an adjacent coronary artery without angioplasty. First case report and review of the literature. Cardiovasc Radiat Med. 5:166–170. 2004. View Article : Google Scholar
10.	Shammas NW, Shammas GA, Hafez A, Kelly R, Reynolds E and Shammas AN: Safety and one-year revascularization outcome of excimer laser ablation therapy in treating in-stent restenosis of femoropopliteal arteries: A retrospective review from a single center. Cardiovasc Revasc Med. 13:341–344. 2012.
11.	Acharya G and Park K: Mechanisms of controlled drug release from drug-eluting stents. Adv Drug Deliv Rev. 58:387–401. 2006. View Article : Google Scholar : PubMed/NCBI
12.	Stettler C, Wandel S, Allemann S, et al: Outcomes associated with drug-eluting and bare-metal stents: a collaborative network meta-analysis. Lancet. 370:937–948. 2007. View Article : Google Scholar : PubMed/NCBI
13.	Chicheł A, Skowronek J, Kubaszewska M and Kanikowski M: Hyperthermia - description of a method and a review of clinical applications. Rep Pract Oncol Radiother. 12:267–275. 2007.
14.	Atienza JM, Guinea GV, Rojo FJ, et al: The influence of pressure and temperature on the behavior of the human aorta and carotid arteries. Rev Esp Cardiol. 60:259–267. 2007.(In Spanish).
15.	Orihara K, Biro S, Hamasaki S, Eto H, Miyata M, Ikeda Y and Tei C: Hyperthermia at 43 degrees C for 2h inhibits the proliferation of vascular smooth muscle cells, but not endothelial cells. J Mol Cell Cardiol. 34:1205–1215. 2002. View Article : Google Scholar : PubMed/NCBI
16.	Raucci F and Di Fiore MM: The reproductive activity in the testis of Podarcis s. sicula involves D-aspartic acid: A study on c-kit receptor protein, tyrosine kinase activity and PCNA protein during annual sexual cycle. Gen Comp Endocrinol. 161:373–383. 2009.PubMed/NCBI
17.	Liang CC, Park AY and Guan JL: In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc. 2:329–333. 2007. View Article : Google Scholar : PubMed/NCBI
18.	Gilchrist RK, Medal R, Shorey WD, Hanselman RC, Parrott JC and Taylor CB: Selective inductive heating of lymph nodes. Ann Surg. 146:596–606. 1957. View Article : Google Scholar : PubMed/NCBI
19.	Thiesen B and Jordan A: Clinical applications of magnetic nanoparticles for hyperthermia. Int J Hyperthermia. 24:467–474. 2008. View Article : Google Scholar
20.	Floren MG, Günther RW and Schmitz-Rode T: Noninvasive inductive stent heating: alternative approach to prevent instent restenosis? Invest Radiol. 39:264–270. 2004. View Article : Google Scholar : PubMed/NCBI
21.	Oya J, Shoji H, Sato F, et al: Thermotherapy with metallic stent excited by the magnetic field. IEEE T Magn. 42:3593–3595. 2006. View Article : Google Scholar
22.	Brasselet C, Durand E, Addad F, et al: Effect of local heating on restenosis and in-stent neointimal hyperplasia in the atherosclerotic rabbit model: a dose-ranging study. Eur Heart J. 29:402–412. 2008. View Article : Google Scholar
23.	Li CJ, Zheng YF and Zhao LC: Heating NiTi stent in magnetic fields and the thermal effect on smooth muscle cells. Key Eng Mater. 288:579–582. 2005.
24.	Liu JY, Zhao LY, Wang YY, Li DY, Tao D, Li LY and Tang JT: Magnetic stent hyperthermia for esophageal cancer: an in vitro investigation in the ECA-109 cell line. Oncol Rep. 27:791–797. 2012.PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

In vitro study on the feasibility of magnetic stent hyperthermia for the treatment of cardiovascular restenosis

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Related Articles