Andrographolide inhibits the activation of NF‑κB and MMP‑9 activity in H3255 lung cancer cells

Luo,Weimin; Liu,Yuefeng; Zhang,Jun; Luo,Xiangyu; Lin,Chenyi; Guo,Jialong

doi:10.3892/etm.2013.1196

September 2013 Volume 6 Issue 3

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September 2013 Volume 6 Issue 3

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Article

Andrographolide inhibits the activation of NF‑κB and MMP‑9 activity in H3255 lung cancer cells

Authors:
- Weimin Luo
- Yuefeng Liu
- Jun Zhang
- Xiangyu Luo
- Chenyi Lin
- Jialong Guo
View Affiliations / Copyright

Affiliations: Department of Cardiothoracic Surgery, Taihe Hospital Affiliated to Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Department of Ophthalmology, People's Hospital Affiliated to Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China
Pages: 743-746
|
Published online on: July 2, 2013

https://doi.org/10.3892/etm.2013.1196
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Abstract

This study aimed to determine the effect of andrographolide (AD) on the growth of H3255 lung cancer cells and its possible impact on the expression and activity of the matrix metalloproteinase (MMP)‑9 protein. H3255 cells were cultured in vitro, and treated with AD (1, 5 or 10 µM) for 24, 48 or 72 h. Cell proliferation was detected using an MTT assay and the expression of MMP‑9 mRNA was measured using a reverse transcription-polymerase chain reaction (RT‑PCR). The activity of MMP‑9 was assessed by gelatin zymography, while the nuclear translocation of the nuclear factor‑κB (NF‑κB) p65 subunit and the phosphorylation of IκB were determined by western blotting. AD inhibited the proliferation of the H3255 cells in a concentration‑ and time‑dependent manner, in addition to downregulating the expression of MMP‑9 mRNA and the activity of MMP‑9. Moreover, AD significantly inhibited the nuclear translocation of the NF‑κB p65 subunit and suppressed IκB phosphorylation. The significant inhibition of H3255 cell proliferation by AD may have been correlated with the reduction in MMP‑9 expression and activity through the inhibition of the phosphorylation of IκB and the translocation of NF‑κB. The results suggest that AD is a promising drug candidate for the treatment of the migration and invasion of malignant tumors.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Andrographolide inhibits the activation of NF‑κB and MMP‑9 activity in H3255 lung cancer cells

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