Expression and activity of the TLR4/NF-κB signaling pathway in mouse intestine following administration of a short-term high-fat diet

Wang,Ning; Wang,Huguo; Yao,Hua; Wei,Qin; Mao,Xin-Min; Jiang,Tao; Xiang,Jing; Dila,Na

doi:10.3892/etm.2013.1214

Expression and activity of the TLR4/NF-κB signaling pathway in mouse intestine following administration of a short-term high-fat diet

Authors:
- Ning Wang
- Huguo Wang
- Hua Yao
- Qin Wei
- Xin-Min Mao
- Tao Jiang
- Jing Xiang
- Na Dila
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Affiliations: Department of No. 1 Cadre Wards Medicine, First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China, Department of Digestive and Vascular Surgery, First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China, Department of Metabolic Disease, First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China, Department of Biomedical Sciences, First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China, Experimental Teaching Demonstration Centre, Pharmaceutical College of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China, Department of Endocrinology, Xinjiang Petroleum Administration Bureau General Hospital, Urumqi, Xinjiang 830011, P.R. China
Published online on: July 10, 2013 https://doi.org/10.3892/etm.2013.1214
Pages: 635-640

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Abstract

Insulin resistance in obesity is associated with chronic systemic low-grade inflammation. Although it has been shown that Toll-like receptor 4 (TLR4) in the liver, muscle and adipose tissue plays an important role in obesity‑associated inflammation and insulin resistance, the effect of TLR4 activation in the intestine has not been investigated. The aim of this study was to explore the activation of the mouse intestinal TLR4/NF-κB signaling pathway following the administration of a short‑term high-fat diet, as well as the function of the signaling pathway in the local enteric inflammatory response. The effect of the high-fat diet on TLR4 activation, NF-κB and phosphorylated IκB (PIκB) activity, and tumor necrosis factor (TNF)-α and IL-6 expression in the intestinal tissues of diet‑induced obese C57BL/6 mice was investigated. The results demonstrated that the high-fat diet induced TLR4 mRNA and protein expression in intestinal tissues. TLR4/NF-κB signaling pathway activation gradually increased as the number of days of high-fat diet administration increased, and peaked on day 7. Additionally, activation of the signaling pathway reduced PIκB expression levels and increased TNF-α and IL-6 expression levels in intestinal tissues. Our results demonstrated that a short-term high-fat diet induces activation of the TLR4/NF-κB signaling pathway in intestinal tissues, which causes local intestinal low-grade inflammation. These data improve our understanding of the molecular events involved in intestinal low-grade inflammation, which may be the triggering factor for chronic systemic low-grade inflammation.

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