Inhibitory role of S‑nitrosoglutathione in the aggregation of frozen platelets, and its effect on the expression of membrane glycoproteins

Wu,Tao; Zhang,Changhong; Wang,Zantao; Liu,Jinghan; Li,Hui; Zhou,Wu; Wang,Shuying

doi:10.3892/etm.2013.1221

September 2013 Volume 6 Issue 3

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September 2013 Volume 6 Issue 3

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Article

Inhibitory role of S‑nitrosoglutathione in the aggregation of frozen platelets, and its effect on the expression of membrane glycoproteins

Authors:
- Tao Wu
- Changhong Zhang
- Zantao Wang
- Jinghan Liu
- Hui Li
- Wu Zhou
- Shuying Wang
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Affiliations: Department of Blood Transfusion, General Hospital of Beijing Military Region, Beijing 100700, P.R. China, Department of Surgery, Liaohe Oilfield General Hospital, Panjin, Liaoyang 124010, P.R. China, Department of Blood Transfusion, Chinese PLA General Hospital, Haidian, Beijing 100853, P.R. China
Pages: 831-839
|
Published online on: July 12, 2013

https://doi.org/10.3892/etm.2013.1221
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Abstract

The aim of this study was to explore the effects of S‑nitrosoglutathione (GSNO) on the aggregation of frozen platelets, the platelet nitric oxide (NO) content and the expression of membrane glycoproteins. The level of frozen platelet aggregation was measured using a platelet aggregation analyzer, while the content of NO was measured by the nitrate reductase method and the expression of frozen platelet membrane glycoproteins was determined by flow cytometry. The level of frozen platelet aggregation was reduced from 35.47±2.93 to 24.43±3.07% following treatment with GSNO. The mean NO concentration in the 32 samples of frozen platelets treated with GSNO was 45.64±6.31 µmol/l, which was significantly higher compared with the concentration in the fresh liquid platelet group. There were no significant differences in the levels of PAC‑1 in the fresh liquid platelet, frozen platelet and GSNO-treated frozen platelet groups; by contrast, significant differences were observed in the CD42b and CD62P levels. The platelet membrane glycoprotein expression levels in the frozen platelet and the GSNO‑treated frozen platelet groups were not significantly different. The results of the study indicate that GSNO has potential as a cryoprotectant, due to its ability to increase the NO concentration in frozen platelets, inhibit platelet aggregation and maintain platelet function. It is likely that the molecular arrangement and structure of the frozen platelets were altered following GSNO treatment, or that the frozen platelets were affected by alternative mechanisms.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Inhibitory role of S‑nitrosoglutathione in the aggregation of frozen platelets, and its effect on the expression of membrane glycoproteins

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