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Article

Changes in expression of four molecular marker proteins and one microRNA in mesothelial cells of the peritoneal dialysate effluent fluid of peritoneal dialysis patients

  • Authors:
    • Lin Zhang
    • Fuyou Liu
    • Youming Peng
    • Lin Sun
    • Guochun Chen
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Second Xiangya Hospital, Institute of Nephrology, Central South University, Changsha, Hunan 410011, P.R. China
  • Pages: 1189-1193
    |
    Published online on: September 2, 2013
       https://doi.org/10.3892/etm.2013.1281
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Abstract

The aim of this study was to detect the expression of microRNA‑200c and epithelial-mesenchymal transition (EMT) in the mesothelial cells of the peritoneal dialysate effluent fluid of peritoneal dialysis (PD) patients, and to investigate the association between microRNA-200c and peritoneal mesothelial cell EMT. Twelve patients who had recently started continuous ambulatory peritoneal dialysis (PD start group) and 16 patients who had been undergoing peritoneal dialysis for >6 months (PD >6 months group) were randomly chosen for the isolation, culture and identification of effluent cells. qPCR and western blot analysis were used to detect the expression levels of microRNA-200c and the levels of four cellular marker proteins, E-cadherin, vimentin, fibronectin (FN) and COL-1, in effluent cells. The results showed that the effluent cells in peritoneal dialysis were peritoneal mesothelial cells. The level of E-cadherin protein expression was significantly lower in the PD >6 months group than in the PD start group, while vimentin, FN and COL-1 protein expression levels were significantly increased in the PD >6 months group. microRNA-200c in the PD >6 months group was significantly downregulated. The E-cadherin protein expression level was significantly decreased and vimentin, FN and COL-1 protein expression levels were significantly increased in the PD >6 months group. The level of microRNA-200c was significantly reduced in the PD > 6 months group, suggesting that microRNA-200c may be associated with EMT.
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1. 

Smit W, Schouten N, van den Berg N, Langedijk MJ, Struijk DG and Krediet RT; Netherlands Ultrafiltration Failure Study Group: Analysis of the prevalence and causes of ultrafiltration failure during long-term peritoneal dialysis: a cross-sectional study. Perit Dial Int. 24:562–570. 2004.

2. 

Kim YL: Update on mechanisms of ultrafiltration failure. Perit Dial Int. 29(Suppl 2): S123–S127. 2009.PubMed/NCBI

3. 

Selgas R, Bajo A, Jiménez-Heffernan JA, Sánchez-Tomero JA, Del Peso G, Aguilera A and López-Cabrera M: Epithelialto-mesenchymal transition of the mesothelial cell - its role in the response of the peritoneum to dialysis. Nephrol Dial Transplant. 21(Suppl 2): ii2–ii7. 2006.PubMed/NCBI

4. 

Aroeira LS, Aguilera A, Sánchez-Tomero JA, et al: Epithelial to mesenchymal transition and peritoneal membrane failure in peritoneal dialysis patients: pathologic significance and potential therapeutic interventions. J Am Soc Nephrol. 18:2004–2013. 2007. View Article : Google Scholar

5. 

Elsurer R, Afsar B, Sezer S and Ozdemir FN: Peritoneal cells at admission: do they have prognostic significance in peritonitis? Ren Fail. 32:335–342. 2010. View Article : Google Scholar : PubMed/NCBI

6. 

Wienholds E, Kloosterman WP, Miska E, et al: MicroRNA expression in zebrafish embryonic development. Science. 309:310–311. 2005. View Article : Google Scholar

7. 

Chen CZ, Li L, Lodish HF and Bartel DP: MicroRNAs modulate hematopoietic lineage differentiation. Science. 303:83–86. 2004. View Article : Google Scholar : PubMed/NCBI

8. 

Kato M, Zhang J, Wang M, et al: MicroRNA-192 in diabetic kidney glomeruli and its function in TGF-beta-induced collagen expression via inhibition of E-box repressors. Proc Natl Acad Sci USA. 104:3432–3437. 2007. View Article : Google Scholar : PubMed/NCBI

9. 

Ogawa T, Iizuka M, Sekiya Y, et al: Suppression of type I collagen production by microRNA-29b in cultured human stellate cells. Biochem Biophys Res Commun. 391:316–321. 2010. View Article : Google Scholar : PubMed/NCBI

10. 

Kong D, Li Y, Wang Z, et al: miR-200 regulates PDGF-D-mediated epithelial-mesenchymal transition, adhesion, and invasion of prostate cancer cells. Stem Cells. 27:1712–1721. 2009. View Article : Google Scholar : PubMed/NCBI

11. 

Sabatel C, Cornet AM, Tabruyn SP, et al: Sprouty1, a new target of the angiostatic agent 16K prolactin, negatively regulates angiogenesis. Mol Cancer. 9:2312010. View Article : Google Scholar : PubMed/NCBI

12. 

Jeffrey SS: Cancer biomarker profiling with microRNAs. Nat Biotechnol. 26:400–401. 2008. View Article : Google Scholar : PubMed/NCBI

13. 

Cortez MA, Bueso-Ramos C, Ferdin J, et al: MicroRNAs in body fluids - the mix of hormones and biomarkers. Nat Rev Clin Oncol. 8:467–477. 2011. View Article : Google Scholar : PubMed/NCBI

14. 

Ge Y, Xiao L, Chen X, et al: MicroRNAs in peritoneal dialysis effluent are promising biomarkers for peritoneal fibrosis in peritoneal dialysis patients. Med Hypotheses. 78:155–156. 2012. View Article : Google Scholar : PubMed/NCBI

15. 

Wang B, Koh P, Winbanks C, et al: miR-200a prevents renal fibrogenesis through repression of TGF-β2 expression. Diabetes. 60:280–287. 2011.PubMed/NCBI

16. 

Yang S, Banerjee S, de Freitas A, et al: Participation of miR-200 in pulmonary fibrosis. Am J Pathol. 180:484–493. 2012. View Article : Google Scholar : PubMed/NCBI

17. 

Iwano M, Plieth D, Danoff TM, et al: Evidence that fibroblasts derive from epithelium during tissue fibrosis. J Clin Invest. 110:341–350. 2002. View Article : Google Scholar : PubMed/NCBI

18. 

Williams JD, Craig KJ, Topley N and Williams GT: Peritoneal dialysis: changes to the structure of the peritoneal membrane and potential for biocompatible solutions. Kidney Int Suppl. 84:S158–S161. 2003. View Article : Google Scholar : PubMed/NCBI

19. 

Williams JD, Craig KJ, Topley N, et al: Peritoneal Biopsy Study Group: Morphologic changes in the peritoneal membrane of patients with renal disease. J Am Soc Nephrol. 13:470–479. 2002.PubMed/NCBI

20. 

Yáñez-Mó M, Lara-Pezzi E, Selgas R, et al: Peritoneal dialysis and epithelial-to-mesenchymal transition of mesothelial cells. N Engl J Med. 348:403–413. 2003.

21. 

Jiménez-Heffernan JA, Aguilera A, Aroeira LS, et al: Immunohistochemical characterization of fibroblast subpopulations in normal peritoneal tissue and in peritoneal dialysis-induced fibrosis. Virchows Arch. 444:247–256. 2004.

22. 

Schilte MN, Loureiro J, Keuning ED, ter Wee PM, Celie JW, Beelen RH and van den Born J: Long-term intervention with heparins in a rat model of peritoneal dialysis. Perit Dial Int. 29:26–35. 2009.PubMed/NCBI

23. 

Margetts PJ, Bonniaud P, Liu L, Hoff CM, Holmes CJ, West-Mays JA and Kelly MM: Transient overexpression of TGF-{beta}1 induces epithelial mesenchymal transition in the rodent peritoneum. J Am Soc Nephrol. 16:425–436. 2005.

24. 

Del Peso G, Jiménez-Heffernan JA, Bajo MA, et al: Epithelial-to-mesenchymal transition of mesothelial cells is an early event during peritoneal dialysis and is associated with high peritoneal transport. Kidney Int Suppl. 108:S26–S33. 2008.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang L, Liu F, Peng Y, Sun L and Chen G: Changes in expression of four molecular marker proteins and one microRNA in mesothelial cells of the peritoneal dialysate effluent fluid of peritoneal dialysis patients. Exp Ther Med 6: 1189-1193, 2013.
APA
Zhang, L., Liu, F., Peng, Y., Sun, L., & Chen, G. (2013). Changes in expression of four molecular marker proteins and one microRNA in mesothelial cells of the peritoneal dialysate effluent fluid of peritoneal dialysis patients. Experimental and Therapeutic Medicine, 6, 1189-1193. https://doi.org/10.3892/etm.2013.1281
MLA
Zhang, L., Liu, F., Peng, Y., Sun, L., Chen, G."Changes in expression of four molecular marker proteins and one microRNA in mesothelial cells of the peritoneal dialysate effluent fluid of peritoneal dialysis patients". Experimental and Therapeutic Medicine 6.5 (2013): 1189-1193.
Chicago
Zhang, L., Liu, F., Peng, Y., Sun, L., Chen, G."Changes in expression of four molecular marker proteins and one microRNA in mesothelial cells of the peritoneal dialysate effluent fluid of peritoneal dialysis patients". Experimental and Therapeutic Medicine 6, no. 5 (2013): 1189-1193. https://doi.org/10.3892/etm.2013.1281
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang L, Liu F, Peng Y, Sun L and Chen G: Changes in expression of four molecular marker proteins and one microRNA in mesothelial cells of the peritoneal dialysate effluent fluid of peritoneal dialysis patients. Exp Ther Med 6: 1189-1193, 2013.
APA
Zhang, L., Liu, F., Peng, Y., Sun, L., & Chen, G. (2013). Changes in expression of four molecular marker proteins and one microRNA in mesothelial cells of the peritoneal dialysate effluent fluid of peritoneal dialysis patients. Experimental and Therapeutic Medicine, 6, 1189-1193. https://doi.org/10.3892/etm.2013.1281
MLA
Zhang, L., Liu, F., Peng, Y., Sun, L., Chen, G."Changes in expression of four molecular marker proteins and one microRNA in mesothelial cells of the peritoneal dialysate effluent fluid of peritoneal dialysis patients". Experimental and Therapeutic Medicine 6.5 (2013): 1189-1193.
Chicago
Zhang, L., Liu, F., Peng, Y., Sun, L., Chen, G."Changes in expression of four molecular marker proteins and one microRNA in mesothelial cells of the peritoneal dialysate effluent fluid of peritoneal dialysis patients". Experimental and Therapeutic Medicine 6, no. 5 (2013): 1189-1193. https://doi.org/10.3892/etm.2013.1281
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