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November 2013 Volume 6 Issue 5

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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Article

Effects of quercetin on the proliferation of breast cancer cells and expression of survivin in vitro

  • Authors:
    • Xiao-Hui Deng
    • Hai-Yan Song
    • Ying-Feng Zhou
    • Guo-Yan Yuan
    • Feng-Jin Zheng
  • View Affiliations / Copyright

    Affiliations: Department of Human Anatomy, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453000, P.R. China, West China School of Preclinical & Forensic Medicine, Chengdu, Sichuan 610041, P.R. China
  • Pages: 1155-1158
    |
    Published online on: September 3, 2013
       https://doi.org/10.3892/etm.2013.1285
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Abstract

Quercetin is a hydrophobic agent with potential anticancer activity. The aim of the present study was to observe the effects of quercetin on the proliferation of the breast cancer cell line MCF-7 and the gene expression of survivin. The molecular mechanism underlying the antiproliferative effect of quercetin was also investigated. MCF-7 breast cancer cells were treated with various concentrations of quercetin. The inhibitory effect of quercetin on proliferation was detected using the 3-(4,5-dimethylthiazol-2-yl)‑2,5-diphenyltetrazolium bromide (MTT) method and the inhibition rate was calculated. Cellular apoptosis was detected by immunocytochemistry and survivin mRNA expression levels were observed using reverse transcription‑polymerase chain reaction (RT-PCR). Western blot analysis was used to analyze changes in the expression levels of survivin protein. Quercetin induced the apoptosis of MCF-7 cells and inhibited the proliferation of the MCF-7 breast cancer cells in a time- and concentration-dependent manner. The mRNA and protein expression levels of survivin were reduced as the concentration of quercetin increased. Quercetin inhibited the growth of MCF-7 cells and promoted apoptosis by inducing G0/G1 phase arrest. It also regulated the expression of survivin mRNA in MCF-7 cells, which may be the mechanism underlying its antitumor effect.
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Copy and paste a formatted citation
Spandidos Publications style
Deng X, Song H, Zhou Y, Yuan G and Zheng F: Effects of quercetin on the proliferation of breast cancer cells and expression of survivin in vitro. Exp Ther Med 6: 1155-1158, 2013.
APA
Deng, X., Song, H., Zhou, Y., Yuan, G., & Zheng, F. (2013). Effects of quercetin on the proliferation of breast cancer cells and expression of survivin in vitro. Experimental and Therapeutic Medicine, 6, 1155-1158. https://doi.org/10.3892/etm.2013.1285
MLA
Deng, X., Song, H., Zhou, Y., Yuan, G., Zheng, F."Effects of quercetin on the proliferation of breast cancer cells and expression of survivin in vitro". Experimental and Therapeutic Medicine 6.5 (2013): 1155-1158.
Chicago
Deng, X., Song, H., Zhou, Y., Yuan, G., Zheng, F."Effects of quercetin on the proliferation of breast cancer cells and expression of survivin in vitro". Experimental and Therapeutic Medicine 6, no. 5 (2013): 1155-1158. https://doi.org/10.3892/etm.2013.1285
Copy and paste a formatted citation
x
Spandidos Publications style
Deng X, Song H, Zhou Y, Yuan G and Zheng F: Effects of quercetin on the proliferation of breast cancer cells and expression of survivin in vitro. Exp Ther Med 6: 1155-1158, 2013.
APA
Deng, X., Song, H., Zhou, Y., Yuan, G., & Zheng, F. (2013). Effects of quercetin on the proliferation of breast cancer cells and expression of survivin in vitro. Experimental and Therapeutic Medicine, 6, 1155-1158. https://doi.org/10.3892/etm.2013.1285
MLA
Deng, X., Song, H., Zhou, Y., Yuan, G., Zheng, F."Effects of quercetin on the proliferation of breast cancer cells and expression of survivin in vitro". Experimental and Therapeutic Medicine 6.5 (2013): 1155-1158.
Chicago
Deng, X., Song, H., Zhou, Y., Yuan, G., Zheng, F."Effects of quercetin on the proliferation of breast cancer cells and expression of survivin in vitro". Experimental and Therapeutic Medicine 6, no. 5 (2013): 1155-1158. https://doi.org/10.3892/etm.2013.1285
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