Monitoring luciferase‑labeled human prostate stem cell antigen‑expressing tumor growth in a mouse model

Dong,Lei; Zhang,Xiaopeng; Yu,Changming; Yu,Ting; Liu,Shuling; Hou,Lihua; Fu,Ling; Yi,Shaoqiong; Chen,Wei

doi:10.3892/etm.2013.1293

Monitoring luciferase‑labeled human prostate stem cell antigen‑expressing tumor growth in a mouse model

Authors:
- Lei Dong
- Xiaopeng Zhang
- Changming Yu
- Ting Yu
- Shuling Liu
- Lihua Hou
- Ling Fu
- Shaoqiong Yi
- Wei Chen
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Affiliations: Clinical Laboratory Center, PLA Air Force General Hospital, Haidian, Beijing 100142, P.R. China, Beijing Institute of Biotechnology, Academy of Military Medical Sciences, Fengtai, Beijing 100071, P.R. China
Published online on: September 13, 2013 https://doi.org/10.3892/etm.2013.1293
Pages: 1208-1212

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Abstract

The aim of this study was to establish a tumor model in mice with the expression of luciferase (Luc) and human prostate stem cell antigen (PSCA), in order to evaluate the activities of anticancer drugs or vaccines for prostate cancer. RM‑1 cells were stably transfected with pcDNA‑Luc and pcDNA‑PSCA plasmids. The Luc‑expressing cells were examined using a luminometer and the PSCA‑expressing cells were examined using a reverse transcription‑polymerase chain reaction (RT‑PCR) and flow cytometric analysis. Male C57BL/6 mice were inoculated subcutaneously with the RM‑PSCA/Luc cells, prior to the tumor growth and survival time of the mice being measured, respectively. In vivo bioluminescence imaging was used to detect Luc expression and immunohistochemical analysis was used to detect PSCA expression. Inoculation of the tumor cells into the C57BL/6 mice closely mimicked the tumor growth of prostate cancer. All of the inoculated mice exhibited a detectable tumor within two weeks. Tumor progression was able to be quantitatively monitored following the inoculation of 1x106 RM‑PSCA/Luc cells. There was an excellent correlation (R2=0.9849) between the photon counts and tumor volume. The expression of PSCA in tumor tissues was confirmed using immunohistochemical analysis. The Luc and PSCA co‑expression tumor model was successfully established in mice, which is likely to accelerate the understanding of the pathogenesis of prostate cancer and facilitate the development of novel antitumor drugs or vaccines for the disease.

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1.	Jemal A, Siegel R, Ward E, Murray T, Xu J and Thun MJ: Cancer statistics, 2007. CA Cancer J Clin. 57:43–66. 2007. View Article : Google Scholar
2.	Hillman GG, Triest JA, Cher ML, Kocheril SV and Talati BR: Prospects of immunotherapy for the treatment of prostate carcinoma - a review. Cancer Detect Prev. 23:333–342. 1999. View Article : Google Scholar : PubMed/NCBI
3.	Crawford ED, Rosenblum M, Ziada AM and Lange PH: Hormone refractory prostate cancer. Urology. 54(Suppl): 1–7. 1999. View Article : Google Scholar
4.	Reiter RE, Gu z, Watabe T, Thomas G, Szigeti K, Davis E, Wahl M, Nisitani S, Yamashiro J, Le Beau MM, Loda M and Witte ON: Prostate stem cell antigen: a cell surface maker over-expressed in prostate cancer. Natl Acad Sci USA. 95:1735–1740. 1998. View Article : Google Scholar : PubMed/NCBI
5.	Antica M, Wu L and Scollay R: Stem cell antigen 2 expression in adult and developing mice. Immunol Lett. 55:47–51. 1997. View Article : Google Scholar : PubMed/NCBI
6.	Nakamura K, Yasunaga Y, Ko D, Xu LL, Moul JW, Peehl DM, Srivastava S and Rhim JS: Cadmium-induced neoplastic transformation of human prostate epithelial cells. Int J Oncol. 20:543–547. 2002.PubMed/NCBI
7.	Gu Z, Thomas G, Yamashiro J, Shintaku IP, Dorey F, Raitano A, Witte ON, Said JW, Loda M and Reiter RE: Prostate stem cell antigen (PSCA) expression increases with high Gleason score, advanced stage and bone metastasis in prostate cancer. Oncogene. 19:1288–1296. 2000. View Article : Google Scholar : PubMed/NCBI
8.	Zhang X, Yu C, Zhao J, Fu L, Yi S, Liu S, Yu T and Chen W: Vaccination with a DNA vaccine based on human PSCA and HSP70 adjuvant enhances the antigen-specific CD8⁺ T-cell response and inhibits the PSCA⁺ tumors growth in mice. J Gene Med. 9:715–726. 2007. View Article : Google Scholar : PubMed/NCBI
9.	Garcia-Hernandez Mde L, Gray A, Hubby B, Klinger OJ and Kast WM: Prostate stem cell antigen vaccination induces a long-term protective immune response against prostate cancer in the absence of autoimmunity. Cancer Res. 68:861–869. 2008.
10.	Ahmad S, Casey G, Cronin M, Rajendran S, Sweeney P, Tangney M and O’Sullivan GC: Induction of effective antitumor response after mucosal bacterial vector mediated DNA vaccinationwith endogenous prostate cancer specific antigen. J Urol. 186:687–693. 2011. View Article : Google Scholar
11.	Kalra J, Anantha M, Warburton C, Waterhouse D, Yan H, Yang YJ, Strut D, Osooly M, Masin D and Bally MB: Validating the use of a luciferase labeled breast cancer cell line, MDA435LCC6, as a means to monitor tumor progression and to assess the therapeutic activity of an established anticancer drug, docetaxel (Dt) alone or in combination with the ILK inhibitor, QLT0267. Cancer Biol Ther. 11:826–838. 2011.
12.	Dithmar S, Rusciano D and Grossniklaus HE: A new technique for implantation of tissue culture melanoma cells in a murine model of metastatic ocular melanoma. Melanoma Res. 10:2–8. 2000. View Article : Google Scholar : PubMed/NCBI
13.	Ntziachristos V, Ripoll J, Wang LV and Weissleder R: Looking and listening to light: the evolution of whole-body photonic imaging. Nat Biotechnol. 23:313–320. 2005. View Article : Google Scholar : PubMed/NCBI
14.	Herschman HR: Molecular imaging: looking at problems, seeing solutions. Science. 302:605–608. 2003. View Article : Google Scholar : PubMed/NCBI
15.	Weissleder R and Ntziachristos V: Shedding light onto live molecular targets. Nat Med. 9:123–128. 2003. View Article : Google Scholar : PubMed/NCBI
16.	Edinger M, Hoffmann P, Contag CH and Negrin RS: Evaluation of effector cell fate and function by in vivo bioluminescence imaging. Methods. 31:172–179. 2003. View Article : Google Scholar : PubMed/NCBI
17.	Dong L, Zhang X, Yi S, Mao Y, Yu T, Hou L, Fu L, Yu C and Chen W: Establishment of a mouse model of human PSCA-expressing prostate cancer. Acta Lab Anim Sci Sin. 17:428–431. 2009.(In Chinese).
18.	Edinger M, Sweeny TJ, Tucker AA, Olomu AB, Negrin RS and Contag CH: Noninvasive assessment of tumor cell proliferation in animal models. Neoplasia. 1:303–310. 1999. View Article : Google Scholar : PubMed/NCBI
19.	Wang X, Rosol M, Ge S, Peterson D, McNamara G, Pollack H, Kohn DB, Nelson MD and Crooks GM: Dynamic tracking of human hematopoietic stem cell engraftment using in vivo bioluminescence imaging. Blood. 102:3478–3482. 2003. View Article : Google Scholar : PubMed/NCBI
20.	Zhang H, Li Y, Wang Z and Zhang B: Establishment of xenograft mouse models to study human lung cancer by using in vivo imaging system. Sheng Wu Gong Cheng Xue Bao. 25:1204–1210. 2009.(In Chinese).