Soluble toll-like receptor 4 reversed attenuating effect of Chinese herbal Xiao-Qing-Long-Tang on allergen induced nerve growth factor and thymic stromal lymphopoietin

  • Authors:
    • Ren‑Shiu Chang
    • Yu‑Chin Wang
    • Shung‑Te Kao
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  • Published online on: September 13, 2013     https://doi.org/10.3892/etm.2013.1294
  • Pages: 1199-1207
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Abstract

Xiao‑Qing‑Long‑Tang (XQLT) is known to regulate allergic immune reactions. The aim of this study was to investigate the effects of XQLT on allergen‑induced cytokines and associated signaling pathways. An acute allergic mouse model was used to investigate the effects of XQLT on nerve growth factor (NGF) during an allergic reaction, while human pulmonary alveolar epithelial cells (HPAEpiCs) were used to investigate the effects of XQLT on Dermatophagoides pteronyssinus group 2 (Der p 2)‑induced NGF, p75 neurotrophin receptor (p75NTR) and thymic stromal lymphopoietin (TSLP) expression. XQLT was demonstrated to inhibit NGF- and p75NTR-related allergic reactions in the mouse model. XQLT also reduced the expression of Toll‑like receptor 4 (TLR4) in the lungs of the model mice. XQLT inhibited Der p 2‑induced NGF, TSLP and p75NTR expression in the HPAEpiC cell line. The use of recombinant soluble TLR4 (sTLR4) in a competitive assay partially attenuated the inhibitory effect of XQLT on NGF, TSLP and p75NTR expression in HPAEpiC cells. The inhibitory effect of XQLT on the Ser536 phosphorylation of p65 (nuclear factor‑κB; NF‑κB), a TLR4‑induced factor, was also attenuated by sTLR4. In conclusion, XQLT inhibited Der p allergen‑induced NGF, p75NTR and TSLP expression. This inhibition was attenuated by sTLR4. The mechanism of action of XQLT may be correlated with TLR4, a primary receptor in the innate immune system. The findings of this study may focus the search for pharmacological targets of XQLT onto TLR4, which is important in the allergen presentation pathway.
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November 2013
Volume 6 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Chang RS, Wang YC and Kao ST: Soluble toll-like receptor 4 reversed attenuating effect of Chinese herbal Xiao-Qing-Long-Tang on allergen induced nerve growth factor and thymic stromal lymphopoietin. Exp Ther Med 6: 1199-1207, 2013
APA
Chang, R., Wang, Y., & Kao, S. (2013). Soluble toll-like receptor 4 reversed attenuating effect of Chinese herbal Xiao-Qing-Long-Tang on allergen induced nerve growth factor and thymic stromal lymphopoietin. Experimental and Therapeutic Medicine, 6, 1199-1207. https://doi.org/10.3892/etm.2013.1294
MLA
Chang, R., Wang, Y., Kao, S."Soluble toll-like receptor 4 reversed attenuating effect of Chinese herbal Xiao-Qing-Long-Tang on allergen induced nerve growth factor and thymic stromal lymphopoietin". Experimental and Therapeutic Medicine 6.5 (2013): 1199-1207.
Chicago
Chang, R., Wang, Y., Kao, S."Soluble toll-like receptor 4 reversed attenuating effect of Chinese herbal Xiao-Qing-Long-Tang on allergen induced nerve growth factor and thymic stromal lymphopoietin". Experimental and Therapeutic Medicine 6, no. 5 (2013): 1199-1207. https://doi.org/10.3892/etm.2013.1294