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Article

Effects of iodine-125 seeds on the methylation of SFRP2 and P16 in colorectal cancer

  • Authors:
    • Xiaogang Li
    • Ping Lu
    • Bo Li
    • Wangfu Zhang
    • Kaiyuan Luo
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The Fourth Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650021, P.R. China, Department of Vascular Surgery, The Fourth Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650021, P.R. China
  • Pages: 1225-1228
    |
    Published online on: September 13, 2013
       https://doi.org/10.3892/etm.2013.1298
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Abstract

The current study investigated the effects of iodine‑125 seeds on the gene methylation of SFRP2 and P16 in colorectal cancer. Mouse models of human colorectal cancer were randomly divided into an experimental group (n=25) and a control group (n=25). The control group was implanted with blank seeds (0 MBq) and the experimental group with iodine-125 seeds (14.8 MBq). At 20 days after implantation, the animals were sacrificed. The methylation levels of SFRP2 and P16 were detected using methylation-specific polymerase chain reactions (MSPs). Following iodine-125 seed irradiation, the level of SFRP2 methylation decreased. The methylation index of the experimental group (0.67±0.05) was significantly lower than that of the control group (0.84±0.07; P<0.05). In the experimental group, 10 samples (40%) displayed methylation in the P16 promoter region compared with 14 (56%) in the control group, which was a significant difference (P<0.05). Iodine-125 seeds induce the downregulation of methylated tumor suppressor gene promoters, thereby inhibiting the proliferation and growth of tumor cells.
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1. 

Kunnumakkara AB, Diagaradjane P, Anand P, et al: Curcumin sensitizes human colorectal cancer to capecitabine by modulation of cyclin D1, COX-2, MMP-9, VEGF and CXCR4 expression in an orthotopic mouse model. Int J Cancer. 125:2187–2197. 2009. View Article : Google Scholar : PubMed/NCBI

2. 

Moriya Y: Treatment strategy for locally recurrent rectal cancer. Jpn J Clin Oncol. 36:127–131. 2006. View Article : Google Scholar : PubMed/NCBI

3. 

Friel CM, Cromwell JW, Marra C, Madoff RD, Rothenberger DA and Garcia-Aguílar J: Salvage radical surgery after failed local excision for early rectal cancer. Dis Colon Rectum. 45:875–879. 2002. View Article : Google Scholar : PubMed/NCBI

4. 

Vermaas M, Ferenschild FT, Verhoef C, et al: Total pelvic exenteration for primary locally advanced and locally recurrent rectal cancer. Eur J Surg Oncol. 33:452–458. 2007. View Article : Google Scholar : PubMed/NCBI

5. 

Guiney MJ, Smith JG, Worotniuk V, Ngan S and Blakey D: Radiotherapy treatment for isolated loco-regional recurrence of rectosigmoid cancer following definitive surgery: Peter MacCallum Cancer Institute experience, 1981–1990. Int J Radiat Oncol Biol Phys. 38:1019–1025. 1997.

6. 

Wang J, Sui A, Jia Y, et al: Treatment of unresectable advanced gastric cancer using iodine-125 brachytherapy. Chin J Clin Oncol. 3:212–215. 2006. View Article : Google Scholar

7. 

Jarusevicius L, Inciura A, Juozaityte E, Vaiciunas K, Vaitkus A and Sniureviciute M: Comparison of implant quality between loose and intra-operatively linked iodine-125 seeds in prostate cancer brachytherapy. J Radiat Res. 53:439–446. 2012.

8. 

Straver ME, Loo CE, Alderliesten T, Rutgers EJ and Vrancken Peeters MT: Marking the axilla with radioactive iodine seeds (MARI procedure) may reduce the need for axillary dissection after neoadjuvant chemotherapy for breast cancer. Br J Surg. 97:1226–1231. 2010. View Article : Google Scholar

9. 

Ma ZH, Yang Y, Zou L and Luo KY: 125I seed irradiation induces up-regulation of the genes associated with apoptosis and cell cycle arrest and inhibits growth of gastric cancer xenografts. J Exp Clin Cancer Res. 31:612012. View Article : Google Scholar

10. 

Luo KY, Shao QH, Yang GK, et al: 125I seed implantation in sphincter preservation for treatment of low rectal cancer. Zhonghua Yi Xue Za Zhi. 85:1355–1357. 2005.(In Chinese).

11. 

Wang JJ, Yuan HS, Li JN, Jiang WJ, Jiang YL and Tian SQ: Interstitial permanent implantation of 125I seeds as salvage therapy for re-recurrent rectal carcinoma. Int J Colorectal Dis. 24:391–399. 2009.PubMed/NCBI

12. 

Ma JX, Jin ZD, Si PR, et al: Continuous and low-energy 125I seed irradiation changes DNA methyltransferases expression patterns and inhibits pancreatic cancer tumor growth. J Exp Clin Cancer Res. 30:352011.PubMed/NCBI

13. 

Waddington CH: Preliminary notes on the development of the wings in normal and mutant strains of drosophila. Proc Natl Acad Sci USA. 25:299–307. 1939. View Article : Google Scholar : PubMed/NCBI

14. 

Esteller M: Epigenetics in cancer. N Engl J Med. 358:1148–1159. 2008. View Article : Google Scholar

15. 

Weber M, Hellmann I, Stadler MB, et al: Distribution, silencing potential and evolutionary impact of promoter DNA methylation in the human genome. Nat Genet. 39:457–466. 2007. View Article : Google Scholar : PubMed/NCBI

16. 

Herman JG, Graff JR, Myöhänen S, Nelkin BD and Baylin SB: Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci USA. 93:9821–9826. 1996. View Article : Google Scholar : PubMed/NCBI

17. 

Löf-Ohlin ZM and Nilsson TK: Pyrosequencing assays to study promoter CpG site methylation of the O6-MGMT, hMLH1, p14ARF, p16INK4a, RASSF1A, and APC1A genes. Oncol Rep. 21:721–729. 2009.PubMed/NCBI

18. 

Jubb AM, Bell SM and Quirke P: Methylation and colorectal cancer. J Pathol. 195:111–134. 2001. View Article : Google Scholar : PubMed/NCBI

19. 

Serrano M, Hannon GJ and Beach D: A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4. Nature. 366:704–707. 1993. View Article : Google Scholar : PubMed/NCBI

20. 

Kaiyuan Luo: Practical Insterstitial Brachytherapy of Malignant tumor. Beijing: The People’s Medical Publishing House; first edition. 2008

21. 

Raiche J, Rodriguez-Juarez R, Pogribny I and Kovalchuk O: Sex- and tissue-specific expression of maintenance and de novo DNA methyltransferases upon low dose X-irradiation in mice. Biochem Biophys Res Commun. 325:39–47. 2004. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li X, Lu P, Li B, Zhang W and Luo K: Effects of iodine-125 seeds on the methylation of SFRP2 and P16 in colorectal cancer. Exp Ther Med 6: 1225-1228, 2013.
APA
Li, X., Lu, P., Li, B., Zhang, W., & Luo, K. (2013). Effects of iodine-125 seeds on the methylation of SFRP2 and P16 in colorectal cancer. Experimental and Therapeutic Medicine, 6, 1225-1228. https://doi.org/10.3892/etm.2013.1298
MLA
Li, X., Lu, P., Li, B., Zhang, W., Luo, K."Effects of iodine-125 seeds on the methylation of SFRP2 and P16 in colorectal cancer". Experimental and Therapeutic Medicine 6.5 (2013): 1225-1228.
Chicago
Li, X., Lu, P., Li, B., Zhang, W., Luo, K."Effects of iodine-125 seeds on the methylation of SFRP2 and P16 in colorectal cancer". Experimental and Therapeutic Medicine 6, no. 5 (2013): 1225-1228. https://doi.org/10.3892/etm.2013.1298
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Lu P, Li B, Zhang W and Luo K: Effects of iodine-125 seeds on the methylation of SFRP2 and P16 in colorectal cancer. Exp Ther Med 6: 1225-1228, 2013.
APA
Li, X., Lu, P., Li, B., Zhang, W., & Luo, K. (2013). Effects of iodine-125 seeds on the methylation of SFRP2 and P16 in colorectal cancer. Experimental and Therapeutic Medicine, 6, 1225-1228. https://doi.org/10.3892/etm.2013.1298
MLA
Li, X., Lu, P., Li, B., Zhang, W., Luo, K."Effects of iodine-125 seeds on the methylation of SFRP2 and P16 in colorectal cancer". Experimental and Therapeutic Medicine 6.5 (2013): 1225-1228.
Chicago
Li, X., Lu, P., Li, B., Zhang, W., Luo, K."Effects of iodine-125 seeds on the methylation of SFRP2 and P16 in colorectal cancer". Experimental and Therapeutic Medicine 6, no. 5 (2013): 1225-1228. https://doi.org/10.3892/etm.2013.1298
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