Inhibition of fibrosis and inflammation by triple therapy with pirfenidone, edaravone and erythropoietin in rabbits with drug-induced lung injury: comparison of CT imaging and pathological findings

  • Authors:
    • Shobu Watanabe
    • Norihisa Nitta
    • Akinaga Sonoda
    • Ayumi Nitta-Seko
    • Shinichi Ohta
    • Keiko Tsuchiya
    • Hideji Otani
    • Yuki Tomozawa
    • Yukihiro Nagatani
    • Kenichi Mukaisho
    • Masashi Takahashi
    • Kiyoshi Murata
  • View Affiliations

  • Published online on: September 18, 2013     https://doi.org/10.3892/etm.2013.1308
  • Pages: 1096-1100
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Abstract

In a rabbit model of bleomycin-induced lung injury, computed tomography (CT) and pathological studies were conducted to investigate whether the progression of this injury is inhibited by pirfenidone and by triple therapy with pirfenidone, edaravone and erythropoietin. We divided nine rabbits with bleomycin-induced lung injury into three equally sized groups. Group 1 served as the control, group 2 received pirfenidone alone and group 3 was treated with pirfenidone, edaravone and erythropoietin. Multidetector CT (MDCT) scans were acquired immediately after the administration of bleomycin, and further scans were performed on days 14 and 28. The area of abnormal opacity was calculated. The rabbit lungs were removed and the size of abnormal areas in macroscopic specimens was calculated and the degree of fibrosis and inflammation in microscopic specimens was scored. In order, the average size of the area of abnormal opacity on CT scans was largest in group 1, followed by groups 2 and 3. On day 28, the area of opacity was significantly smaller in group 3 than in group 1 (P=0.071). The average size of the area of abnormal opacity on macroscopic findings was largest in group 1, followed in order by groups 2 and 3; the difference between group 1 and 3 was significant (P<0.05). The average fibrosis score was highest in group 3 followed by groups 2 and 1. By contrast, the average inflammation score was highest in group 2 followed by groups 1 and 3. Although the administration of pirfenidone alone slowed the progression of bleomycin-induced lung injury, the triple-drug combination was more effective.
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November 2013
Volume 6 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Watanabe S, Nitta N, Sonoda A, Nitta-Seko A, Ohta S, Tsuchiya K, Otani H, Tomozawa Y, Nagatani Y, Mukaisho K, Mukaisho K, et al: Inhibition of fibrosis and inflammation by triple therapy with pirfenidone, edaravone and erythropoietin in rabbits with drug-induced lung injury: comparison of CT imaging and pathological findings. Exp Ther Med 6: 1096-1100, 2013
APA
Watanabe, S., Nitta, N., Sonoda, A., Nitta-Seko, A., Ohta, S., Tsuchiya, K. ... Murata, K. (2013). Inhibition of fibrosis and inflammation by triple therapy with pirfenidone, edaravone and erythropoietin in rabbits with drug-induced lung injury: comparison of CT imaging and pathological findings. Experimental and Therapeutic Medicine, 6, 1096-1100. https://doi.org/10.3892/etm.2013.1308
MLA
Watanabe, S., Nitta, N., Sonoda, A., Nitta-Seko, A., Ohta, S., Tsuchiya, K., Otani, H., Tomozawa, Y., Nagatani, Y., Mukaisho, K., Takahashi, M., Murata, K."Inhibition of fibrosis and inflammation by triple therapy with pirfenidone, edaravone and erythropoietin in rabbits with drug-induced lung injury: comparison of CT imaging and pathological findings". Experimental and Therapeutic Medicine 6.5 (2013): 1096-1100.
Chicago
Watanabe, S., Nitta, N., Sonoda, A., Nitta-Seko, A., Ohta, S., Tsuchiya, K., Otani, H., Tomozawa, Y., Nagatani, Y., Mukaisho, K., Takahashi, M., Murata, K."Inhibition of fibrosis and inflammation by triple therapy with pirfenidone, edaravone and erythropoietin in rabbits with drug-induced lung injury: comparison of CT imaging and pathological findings". Experimental and Therapeutic Medicine 6, no. 5 (2013): 1096-1100. https://doi.org/10.3892/etm.2013.1308