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Article

Advanced glycation end‑products reduce podocyte adhesion by activating the renin‑angiotensin system and increasing integrin‑linked kinase

  • Authors:
    • Cailian Cheng
    • Zhenda Zheng
    • Chenggang Shi
    • Xun Liu
    • Zengchun Ye
    • Tanqi Lou
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, The Third Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510630, P.R. China, Department of Cardiology, The Third Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510630, P.R. China
  • Pages: 1494-1498
    |
    Published online on: September 23, 2013
       https://doi.org/10.3892/etm.2013.1312
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Abstract

The aim of this study was to investigate the effects of advanced glycation end‑products (AGEs) on podocyte adhesion and the underlying mechanisms. Immortalized mouse podocytes were exposed to various conditions and podocyte adhesion was evaluated using a hexosaminidase assay. The expression levels of integrin‑linked kinase (ILK) were measured by quantitative polymerase chain reaction (qPCR) and western blotting. Treatment with AGEs resulted in a significant, concentration‑dependent reduction in podocyte adhesion (P<0.05) and an incremental rise in ILK expression up to a maximum of 100%. Pretreatment with losartan significantly prevented the upregulation of ILK and attenuated the loss of podocyte adhesion observed in podocytes exposed to AGEs (P<0.05). However, the adhesion of losartan‑treated podocytes remained lower than that of the podocytes exposed to bovine serum albumin. The results indicate that AGEs reduce podocyte adhesion via the upregulation of ILK expression, which occurs partly through activation of the renin‑angiotensin system in podocytes.
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Copy and paste a formatted citation
Spandidos Publications style
Cheng C, Zheng Z, Shi C, Liu X, Ye Z and Lou T: Advanced glycation end‑products reduce podocyte adhesion by activating the renin‑angiotensin system and increasing integrin‑linked kinase. Exp Ther Med 6: 1494-1498, 2013.
APA
Cheng, C., Zheng, Z., Shi, C., Liu, X., Ye, Z., & Lou, T. (2013). Advanced glycation end‑products reduce podocyte adhesion by activating the renin‑angiotensin system and increasing integrin‑linked kinase. Experimental and Therapeutic Medicine, 6, 1494-1498. https://doi.org/10.3892/etm.2013.1312
MLA
Cheng, C., Zheng, Z., Shi, C., Liu, X., Ye, Z., Lou, T."Advanced glycation end‑products reduce podocyte adhesion by activating the renin‑angiotensin system and increasing integrin‑linked kinase". Experimental and Therapeutic Medicine 6.6 (2013): 1494-1498.
Chicago
Cheng, C., Zheng, Z., Shi, C., Liu, X., Ye, Z., Lou, T."Advanced glycation end‑products reduce podocyte adhesion by activating the renin‑angiotensin system and increasing integrin‑linked kinase". Experimental and Therapeutic Medicine 6, no. 6 (2013): 1494-1498. https://doi.org/10.3892/etm.2013.1312
Copy and paste a formatted citation
x
Spandidos Publications style
Cheng C, Zheng Z, Shi C, Liu X, Ye Z and Lou T: Advanced glycation end‑products reduce podocyte adhesion by activating the renin‑angiotensin system and increasing integrin‑linked kinase. Exp Ther Med 6: 1494-1498, 2013.
APA
Cheng, C., Zheng, Z., Shi, C., Liu, X., Ye, Z., & Lou, T. (2013). Advanced glycation end‑products reduce podocyte adhesion by activating the renin‑angiotensin system and increasing integrin‑linked kinase. Experimental and Therapeutic Medicine, 6, 1494-1498. https://doi.org/10.3892/etm.2013.1312
MLA
Cheng, C., Zheng, Z., Shi, C., Liu, X., Ye, Z., Lou, T."Advanced glycation end‑products reduce podocyte adhesion by activating the renin‑angiotensin system and increasing integrin‑linked kinase". Experimental and Therapeutic Medicine 6.6 (2013): 1494-1498.
Chicago
Cheng, C., Zheng, Z., Shi, C., Liu, X., Ye, Z., Lou, T."Advanced glycation end‑products reduce podocyte adhesion by activating the renin‑angiotensin system and increasing integrin‑linked kinase". Experimental and Therapeutic Medicine 6, no. 6 (2013): 1494-1498. https://doi.org/10.3892/etm.2013.1312
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