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Article

Evidence of an anti-apoptotic effect of qinghuobaiduyin on intestinal mucosa following burn injury

  • Authors:
    • Jie Zhu
    • Ping Wang
    • Quanyong He
    • Jianda Zhou
    • Chengqun Luo
  • View Affiliations / Copyright

    Affiliations: Department of Burn and Plastics Surgery, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China, PET/CT Center, Hunan Provincial Tumor Hospital, Changsha, Hunan 410013, P.R. China
  • Pages: 1390-1396
    |
    Published online on: September 26, 2013
       https://doi.org/10.3892/etm.2013.1314
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Abstract

Burn injuries are common in wartime and in times of peace. The prevention and therapy of ischemia‑reperfusion injury to the organs, in particular the intestine, during the burn shock and recovery process has become a popular yet challenging area of research. Studies concerning the apoptosis of the cells of the burned intestinal mucosa have gained considerable attention. Qinghuobaiduyin (QHBDY) is a traditional Chinese medicine that has been used as a clinical prescription since 1995 to treat burn patients due to its opsonization function in the immune system and favorable clinical therapeutic effect. The aim of this study was to investigate the effect of QHBDY on the apoptosis of intestinal mucosa following burn injury. An animal model was constructed comprising severely burned rats that were treated with various dosages of QHBDY. Tissues from the small intestine were collected to investigate the apoptosis rate by TUNEL assay and the protein expression levels of heat shock protein 70 (Hsp70) and caspase-3 by immunohistochemistry. In addition, IEC-18 cells treated with QHBDY and burn serum were investigated. The cell apoptosis rate was analyzed by flow cytometry (FCM), the protein expression levels of Hsp70 were measured by western blot analysis and caspase-3 activity was analyzed by a colorimetric assay. The results showed that in animal experiments, compared with the burned group, the apoptosis rates in the treatment group was decreased, the protein expression level of Hsp70 was increased while Caspase-3 was decreased. In cell experiments, after treatment with QHBDY, the cell apoptosis rate was lower than that of the burn serum group. In addition, Hsp70 protein expression was upregulated and caspase-3 activity was decreased. QHBDY may play an important role in the prevention of apoptosis at the whole animal and cellular levels.
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View References

1 

Itoh H, Yagi M, Fushida S, et al: Activation of immediate early gene, c-fos, and c-jun in the rat small intestine after ischemia/reperfusion. Transplantion. 69:598–604. 2000. View Article : Google Scholar : PubMed/NCBI

2 

Wang L, Zhou Y, Peng J, Zhang Z, Jiang DJ and Li YJ: Role of endogenous nitric oxide synthase inhibitor in gastric mucosal injury. Can J Physiol Pharmacol. 86:97–104. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Kumar V, Mohanty MK and Kanth S: Fatal burns in Manipal area: a 10 year study. J Forensic Leg Med. 14:3–6. 2007.PubMed/NCBI

4 

Shinozawa Y: Fluid management and care for multiple organ dysfunction syndrome in patients with extensive burns. Nippon Geka Gakkai Zasshi. 106:736–739. 2005.(In Japanese).

5 

Duncan DJ, Hopkins PM and Harrison SM: Negative inotropic effect of tumour necrosis factor-alpha and interleukin-1beta are ameliorated by alfentanil in rat ventricular myocytes. Br J Pharmacol. 150:720–726. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Lin WJ, Wang DL and Pan YQ: Depression research of psychoneuroimmunology: the role of cytokines. Adv Psychol Sci. 16:404–410. 2008.(In Chinese).

7 

Cheng C, Li Z, Huang W, et al: The influence of the large military exercise on the soldiers’ some immune-endocrine function. Med J Chin PLA. 32:189–190. 2007.(In Chinese).

8 

Mishima S, Yukioka T, Matsuda H and Shimazaki S: Mild hypotension and body burns synergistically increase bacterial translocation in rats consistent with a ‘two-hit phenomenon’. J Burn Care Rehabil. 18:22–26. 1997.PubMed/NCBI

9 

Zhang HY, Lv NH, Xie Y, et al: Protection of heat shock preconditioning on acute gastric mucosal lesion in scalded rats and its mechanism. Zhonghua Shao Shang Za Zhi. 23:58–61. 2007.(In Chinese).

10 

Odashima M, Otaka M, Jin M, et al: Induction of a 72-kDa heat-shock protein in cultured rat gastric mucosal cells and rat gastric mucosa by zinc L-carnosine. Dig Dis Sci. 47:2799–2804. 2002. View Article : Google Scholar : PubMed/NCBI

11 

Muñoz J, Albillos A, Pérez-Páramo M, Rossi I and Alvarez-Mon M: Factors mediating the hemodynamic effects of tumor necrosis factor-alpha in portal hypertensive rats. Am J Physiol. 276:G687–G693. 1999.PubMed/NCBI

12 

Suemasu S, Tanaka K, Namba T, Ishihara T, Katsu T, Fujimoto M, Adachi H, Sobue G, Takeuchi K, Nakai A and Mizushima T: A role for HSP70 in protecting against indomethacin-induced gastric lesions. J Biol Chem. 284:19705–19715. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Walker HL and Mason AD Jr: A standard animal burn. J Trauma. 8:1049–1051. 1968. View Article : Google Scholar : PubMed/NCBI

14 

Bromberg Z, Raj N, Goloubinoff P, Deutschman CS and Weiss YG: Enhanced expression of 70-kilodalton heat shock protein limits cell division in a sepsis-induced model of acute respiratory distress syndrome. Crit Care Med. 36:246–255. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Wilmore DW and Robinson MK: Metabolism and nutrition support. Surgical Basic Science. Fischer JE: 7th Edition. Mosby Press Co; St Louis, MO: pp. 1251993

16 

Wang LC: Research progress on chemical compositions and efficacy of Lonicera spp. J Anhui Agric Sci. 37:2036–2037. 2009.(In Chinese).

17 

Wang L, Cui B and Zhang H: Pharmacological research progress on Lonicera spp. Chin J Anim Husbandry Vet Med. 34:91–95. 2007.(In Chinese).

18 

He X and Lan R: Pharmacological action and clinical application of honeysuckle. Lishizhen Med Mat Med Res. 15:865–867. 2004.(In Chinese).

19 

Gong ZZ, Zheng YX, Zheng NG, et al: Anti-oxidative effect of honeysuckle in vivo: an experimental study. Prac J Med Pharm. 23:584–585. 2006.(In Chinese).

20 

Kang OH, Choi JG, Lee JH and Kwon DY: Luteolin isolated from the flowers of Lonicera japonica suppresses inflammatory mediator release by blocking NF-κB and MAPKs activation pathways in HMC-1 cells. Molecules. 15:385–398. 2010.PubMed/NCBI

21 

Xiang Z and Ning Z: Scavenging and antioxidant properties of compound derived from chlorogenic acid in South-China honeysuckle. LWT - Food Sci Technol. 41:1189–1203. 2008. View Article : Google Scholar

22 

Schierenbeck KA: Japanese honeysuckle (Lonicera japonica) as an invasive species; history, ecology, and context. Crit Rev Plant Sci. 23:3912004.

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Copy and paste a formatted citation
Spandidos Publications style
Zhu J, Wang P, He Q, Zhou J and Luo C: Evidence of an anti-apoptotic effect of qinghuobaiduyin on intestinal mucosa following burn injury. Exp Ther Med 6: 1390-1396, 2013.
APA
Zhu, J., Wang, P., He, Q., Zhou, J., & Luo, C. (2013). Evidence of an anti-apoptotic effect of qinghuobaiduyin on intestinal mucosa following burn injury. Experimental and Therapeutic Medicine, 6, 1390-1396. https://doi.org/10.3892/etm.2013.1314
MLA
Zhu, J., Wang, P., He, Q., Zhou, J., Luo, C."Evidence of an anti-apoptotic effect of qinghuobaiduyin on intestinal mucosa following burn injury". Experimental and Therapeutic Medicine 6.6 (2013): 1390-1396.
Chicago
Zhu, J., Wang, P., He, Q., Zhou, J., Luo, C."Evidence of an anti-apoptotic effect of qinghuobaiduyin on intestinal mucosa following burn injury". Experimental and Therapeutic Medicine 6, no. 6 (2013): 1390-1396. https://doi.org/10.3892/etm.2013.1314
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu J, Wang P, He Q, Zhou J and Luo C: Evidence of an anti-apoptotic effect of qinghuobaiduyin on intestinal mucosa following burn injury. Exp Ther Med 6: 1390-1396, 2013.
APA
Zhu, J., Wang, P., He, Q., Zhou, J., & Luo, C. (2013). Evidence of an anti-apoptotic effect of qinghuobaiduyin on intestinal mucosa following burn injury. Experimental and Therapeutic Medicine, 6, 1390-1396. https://doi.org/10.3892/etm.2013.1314
MLA
Zhu, J., Wang, P., He, Q., Zhou, J., Luo, C."Evidence of an anti-apoptotic effect of qinghuobaiduyin on intestinal mucosa following burn injury". Experimental and Therapeutic Medicine 6.6 (2013): 1390-1396.
Chicago
Zhu, J., Wang, P., He, Q., Zhou, J., Luo, C."Evidence of an anti-apoptotic effect of qinghuobaiduyin on intestinal mucosa following burn injury". Experimental and Therapeutic Medicine 6, no. 6 (2013): 1390-1396. https://doi.org/10.3892/etm.2013.1314
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