Effect of traditional Chinese medicine (Xiaochaihu Tang) on the expression of MMP‑2 and MMP‑9 in rats with endometriosis

Jiao,Luyang; Qi,Xiaofen; Lu,Guangjian; Zhang,Qunmei; Zhang,Chunxiao; Gao,Jianhui

doi:10.3892/etm.2013.1316

December 2013 Volume 6 Issue 6

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December 2013 Volume 6 Issue 6

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Article

Effect of traditional Chinese medicine (Xiaochaihu Tang) on the expression of MMP‑2 and MMP‑9 in rats with endometriosis

Authors:
- Luyang Jiao
- Xiaofen Qi
- Guangjian Lu
- Qunmei Zhang
- Chunxiao Zhang
- Jianhui Gao
View Affiliations / Copyright

Affiliations: Department of Laboratory, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China, Department of Emergency, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China, Department of Blood Transfusion, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China, Department of Tuberculosis III, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China, Department of Science and Technology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China
Pages: 1385-1389
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Published online on: September 26, 2013

https://doi.org/10.3892/etm.2013.1316
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Abstract

The aim of this study was to explore the effect of a traditional Chinese medicine (Xiaochaihu Tang, XCHT) on the expression of matrix metalloproteinase‑2 (MMP‑2) and MMP‑9 in rats with endometriosis (EMs). A total of 48 specific‑pathogen‑free (SPF) female Sprague‑Dawley (SD) rats were randomly divided into control (n=8) and EMs (n=40) groups. The EMs model was established using a surgical procedure. At 21 days, the rats with EMs were screened and divided into four subgroups (n=8): the model control, low‑dose (7.5 g/kg) XCHT‑treated, high‑dose (15 g/kg) XCHT‑treated and gestrinone‑treated (0.5 mg/kg) groups. Following 21 days of treatment, the rats were sacrificed. Reverse transcription‑polymerase chain reaction (RT‑PCR) and western blotting were used to examine the mRNA and protein levels of MMP‑2 and MMP‑9 in the endometrium. The expression levels of MMP‑2 and MMP‑9 were significantly increased in the rats with EMs compared with those in normal rats. Moreover, XCHT was able to significantly inhibit the expression of MMP‑2 and MMP‑9 compared with that in the model control group. In conclusion, XCHT was able to decrease the expression of MMP‑2 and MMP‑9 in the ectopic endometrium. The present results may provide a potential theoretical basis for the therapy of EMs.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effect of traditional Chinese medicine (Xiaochaihu Tang) on the expression of MMP‑2 and MMP‑9 in rats with endometriosis

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