Effect of whey protein on plasma amino acids in diabetic mice

Han,Ting; Cai,Donglian; Geng,Shanshan; Wang,Ying; Zhen,Hui; Wu,Peiying

doi:10.3892/etm.2013.1329

Effect of whey protein on plasma amino acids in diabetic mice

Authors:
- Ting Han
- Donglian Cai
- Shanshan Geng
- Ying Wang
- Hui Zhen
- Peiying Wu
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Affiliations: Department of Clinical Nutrition, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Tongji University, Shanghai 200072, P.R. China, Department of Clinical Nutrition, Changhai Hospital, The Second Military Medical University, Shanghai 200433, P.R. China, Department of Clinical Nutrition, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, P.R. China
Published online on: October 7, 2013 https://doi.org/10.3892/etm.2013.1329
Pages: 1449-1454

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Abstract

The aim of this study was to investigate the effect of whey protein on plasma amino acid levels in a mouse model of type II diabetes, using high‑performance liquid chromatography (HPLC). The composition and content of amino acids in the whey proteins were analyzed using HPLC. Type I and type II diabetic mouse models were prepared using streptozotocin (STZ) and normal mice were used as a control. The ICR mice in each group were then randomly divided into four subgroups, to which 0, 10, 20 and 40% whey protein, respectively, was administered for four weeks. Changes in the plasma amino acid levels were observed in each group. The proportions of leucine, isoleucine and valine in the whey proteins were 14.40, 5.93 and 5.32% of the total amino acids, respectively, that is, the branched‑chain amino acid content was 25.65%. The levels of branched‑chain amino acids increased in the plasma of the normal and model mice following the administration of whey proteins by gavage and the amino acid levels increased as the concentration of the administered protein increased. In addition, the branched‑chain amino acid levels in the blood of the model mice were higher than those in the normal mice. The levels of plasma amino acids in diabetic mice increased following gavage with whey protein, which is rich in branched‑chain amino acids.

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