CD4+CD25+FoxP3+ regulatory T cells and cytokines interact with estradiol in cases of missed abortion

Cao,Weiping; Xu,Wenlin; Chen,Tinmei; Wang,Xiaoying; Wang,Xinzhi; Qiu,Jian; Chen,Nintao; Mao,Yu

doi:10.3892/etm.2013.1422

2014-February Volume 7 Issue 2

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2014-February Volume 7 Issue 2

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Article

CD4+CD25+FoxP3+ regulatory T cells and cytokines interact with estradiol in cases of missed abortion

Authors:
- Weiping Cao
- Wenlin Xu
- Tinmei Chen
- Xiaoying Wang
- Xinzhi Wang
- Jian Qiu
- Nintao Chen
- Yu Mao
View Affiliations / Copyright

Affiliations: Department of Maternity and Child Health, Maternity and Child Health Hospital of Zhenjiang, Zhenjiang, Jiangsu, P.R. China, Central Laboratory of Medicine, Maternity and Child Health Hospital of Zhenjiang, Zhenjiang, Jiangsu, P.R. China, Department of Obstetrics, Maternity and Child Health Hospital of Zhenjiang, Zhenjiang, Jiangsu, P.R. China, Jiangsu Center for Drug Screening, China Pharmaceutical University, Nanjing, Jiangsu, P.R. China, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, P.R. China
Pages: 417-422
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Published online on: November 25, 2013

https://doi.org/10.3892/etm.2013.1422
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Abstract

The aim of this study was to examine the interaction of estradiol (E2) with CD4+CD25+FoxP3+ regulatory T (Treg) cells and cytokines in cases of missed abortion (MA). The peripheral blood lymphocytes from patients with MA and controls (normal pregnancy and non-pregnant females) were isolated from the blood by Ficoll density gradient centrifugation. CD4+CD25+ Treg cells were isolated from peripheral blood mononuclear cells (PBMCs). The frequencies of CD4+CD25+FoxP3+ Treg cells and mRNA expression of transcription factor forkhead box protein 3 (FoxP3) in the peripheral blood of MA (n=33), normal pregnancy (n=33) and non-pregnant females (n=27) were determined by intracellular three-color flow cytometry and quantitative polymerase chain reaction (qPCR), respectively. The serum levels of interferon-γ (IFN-γ) and interleukin-4 (IL-4) were measured by enzyme-linked immunosorbent assay (ELISA) and a chemiluminescent immunoassay was used to examine the serum E2 levels. It was observed that the percentage of Foxp3+ T cells in the peripheral blood of patients with MA were lower compared with those in the normal pregnancy and healthy non-pregnant controls; The results demonstrated that MA patients exhibited decreased levels of a peripheral Th2-related cytokine (IL-4) and E2. Furthermore, the low levels of CD4+CD25+Foxp3+ T cells and IL-4 correlated positively with serum concentrations of E2. The data indicated that maternal immunological changes may reverse maternal tolerance in MA, and this phenomenon may be due to the interaction of E2 with CD4+CD25+Foxp3+ T cells and cytokines in MA.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

CD4+CD25+FoxP3+ regulatory T cells and cytokines interact with estradiol in cases of missed abortion

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