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Article

Proapoptotic effect and the mechanism of action of pingyangmycin on cavernous hemangiomas

  • Authors:
    • Yideng Huang
    • Ping Li
    • Siwen Xia
    • Yang Zhuo
    • Longjun Wu
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology, The 118th Hospital of Chinese PLA, Wenzhou, Zhejiang 325000, P.R. China, Department of Otorhinolaryngology, General Hospital of Chengdu Military Region of PLA, Chengdu, Sichuan 610083, P.R. China
  • Pages: 473-477
    |
    Published online on: November 26, 2013
       https://doi.org/10.3892/etm.2013.1428
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Abstract

This study aimed to investigate the proapoptotic effects and the mechanism of action of pingyangmycin (PY) on cavernous hemangioma. The rat spleen was used as a model of cavernous hemangioma. PY was injected into the spleen and the pathological changes were observed at different time‑points. Apoptosis was detected using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and transmission electron microscopy (TEM). The expression levels of the apoptosis-related protein, caspase-3, were determined using immunohistochemistry and image analysis. Rats injected with normal saline were the control group. Injection of normal saline did not damage rat spleens. On days 2 and 5 following PY injection, the spleens exhibited slight swelling. On days 8 and 14, atrophic changes were observed and the splenic sinus endothelial cells were damaged. At various time‑points following PY injection, the apoptotic cells were observed by TEM. The TUNEL assay showed that apoptosis occurred widely among the splenic sinus endothelial cells and other splenic cells. The apoptotic rate and caspase-3 expression levels increased with prolonged PY exposure. PY induced apoptosis of splenic sinus endothelial cells through the caspase-3 activation pathway, and resulted in endothelial cell necrosis and fibroblast hyperplasia.
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Copy and paste a formatted citation
Spandidos Publications style
Huang Y, Li P, Xia S, Zhuo Y and Wu L: Proapoptotic effect and the mechanism of action of pingyangmycin on cavernous hemangiomas. Exp Ther Med 7: 473-477, 2014.
APA
Huang, Y., Li, P., Xia, S., Zhuo, Y., & Wu, L. (2014). Proapoptotic effect and the mechanism of action of pingyangmycin on cavernous hemangiomas. Experimental and Therapeutic Medicine, 7, 473-477. https://doi.org/10.3892/etm.2013.1428
MLA
Huang, Y., Li, P., Xia, S., Zhuo, Y., Wu, L."Proapoptotic effect and the mechanism of action of pingyangmycin on cavernous hemangiomas". Experimental and Therapeutic Medicine 7.2 (2014): 473-477.
Chicago
Huang, Y., Li, P., Xia, S., Zhuo, Y., Wu, L."Proapoptotic effect and the mechanism of action of pingyangmycin on cavernous hemangiomas". Experimental and Therapeutic Medicine 7, no. 2 (2014): 473-477. https://doi.org/10.3892/etm.2013.1428
Copy and paste a formatted citation
x
Spandidos Publications style
Huang Y, Li P, Xia S, Zhuo Y and Wu L: Proapoptotic effect and the mechanism of action of pingyangmycin on cavernous hemangiomas. Exp Ther Med 7: 473-477, 2014.
APA
Huang, Y., Li, P., Xia, S., Zhuo, Y., & Wu, L. (2014). Proapoptotic effect and the mechanism of action of pingyangmycin on cavernous hemangiomas. Experimental and Therapeutic Medicine, 7, 473-477. https://doi.org/10.3892/etm.2013.1428
MLA
Huang, Y., Li, P., Xia, S., Zhuo, Y., Wu, L."Proapoptotic effect and the mechanism of action of pingyangmycin on cavernous hemangiomas". Experimental and Therapeutic Medicine 7.2 (2014): 473-477.
Chicago
Huang, Y., Li, P., Xia, S., Zhuo, Y., Wu, L."Proapoptotic effect and the mechanism of action of pingyangmycin on cavernous hemangiomas". Experimental and Therapeutic Medicine 7, no. 2 (2014): 473-477. https://doi.org/10.3892/etm.2013.1428
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