Proapoptotic effect and the mechanism of action of pingyangmycin on cavernous hemangiomas

  • Authors:
    • Yideng Huang
    • Ping Li
    • Siwen Xia
    • Yang Zhuo
    • Longjun Wu
  • View Affiliations

  • Published online on: November 26, 2013     https://doi.org/10.3892/etm.2013.1428
  • Pages: 473-477
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

This study aimed to investigate the proapoptotic effects and the mechanism of action of pingyangmycin (PY) on cavernous hemangioma. The rat spleen was used as a model of cavernous hemangioma. PY was injected into the spleen and the pathological changes were observed at different time‑points. Apoptosis was detected using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and transmission electron microscopy (TEM). The expression levels of the apoptosis-related protein, caspase-3, were determined using immunohistochemistry and image analysis. Rats injected with normal saline were the control group. Injection of normal saline did not damage rat spleens. On days 2 and 5 following PY injection, the spleens exhibited slight swelling. On days 8 and 14, atrophic changes were observed and the splenic sinus endothelial cells were damaged. At various time‑points following PY injection, the apoptotic cells were observed by TEM. The TUNEL assay showed that apoptosis occurred widely among the splenic sinus endothelial cells and other splenic cells. The apoptotic rate and caspase-3 expression levels increased with prolonged PY exposure. PY induced apoptosis of splenic sinus endothelial cells through the caspase-3 activation pathway, and resulted in endothelial cell necrosis and fibroblast hyperplasia.
View Figures
View References

Related Articles

Journal Cover

2014-February
Volume 7 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Huang Y, Li P, Xia S, Zhuo Y and Wu L: Proapoptotic effect and the mechanism of action of pingyangmycin on cavernous hemangiomas. Exp Ther Med 7: 473-477, 2014
APA
Huang, Y., Li, P., Xia, S., Zhuo, Y., & Wu, L. (2014). Proapoptotic effect and the mechanism of action of pingyangmycin on cavernous hemangiomas. Experimental and Therapeutic Medicine, 7, 473-477. https://doi.org/10.3892/etm.2013.1428
MLA
Huang, Y., Li, P., Xia, S., Zhuo, Y., Wu, L."Proapoptotic effect and the mechanism of action of pingyangmycin on cavernous hemangiomas". Experimental and Therapeutic Medicine 7.2 (2014): 473-477.
Chicago
Huang, Y., Li, P., Xia, S., Zhuo, Y., Wu, L."Proapoptotic effect and the mechanism of action of pingyangmycin on cavernous hemangiomas". Experimental and Therapeutic Medicine 7, no. 2 (2014): 473-477. https://doi.org/10.3892/etm.2013.1428