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Article

A peroxynitrite decomposition catalyst prevents mechanical allodynia and NMDA receptor activation in the hind-paw ischemia reperfusion injury rats

  • Authors:
    • Kyung-Hwa Kwak
    • Hoon Jung
    • Jun Mo Park
    • Jin-Seok Yeo
    • Hyunjee Kim
    • Hyung Chul Lee
    • Sung Hye Byun
    • Jong-Chan Kim
    • Sung-Sik Park
    • Dong Gun Lim
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology and Pain Medicine, Kyungpook National University Hospital, Daegu 700-721, Republic of Korea, Department of Anesthesiology and Pain Medicine, Keimyung University Dongsan Medical Center, Daegu 700‑721, Republic of Korea
  • Pages: 508-512
    |
    Published online on: December 9, 2013
       https://doi.org/10.3892/etm.2013.1440
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Abstract

The contributions of superoxide and nitric oxide to ischemia/reperfusion (I/R)‑induced neuropathic pain have previously been demonstrated in an animal model that mimics the symptoms of complex regional pain syndrome type I (CRPS I). Targeting peroxynitrite, which is the product of their interaction, may provide effective treatments for I/R‑induced neuropathic pain. In this study, the effect of the peroxynitrite decomposition catalyst FeTMPyP [5,10,15,20‑tetrakis (N‑methyl‑4'‑pyridyl)porphyrinato iron (III)], administered at doses of 1, 3 and 10 mg/kg via intraperitoneal injection 30 min prior to reperfusion, was evaluated in rats with chronic post‑ischemic pain. The pain behavior of the rats was tested with a von Frey filament. Phosphorylation of N‑methyl‑D‑aspartate (NMDA) receptors in the L4/6 section of the spinal cord was measured on the third day following reperfusion by western blotting. The rats treated with 3 or 10 mg/kg FeTMPyP demonstrated significant increases in their paw withdrawal thresholds and decreased levels of phosphorylated NMDA receptor subunit 1 compared with those of the vehicle group (all P<0.001). These findings suggest that nitrosative stress, specifically that associated with peroxynitrite, may be involved in the mechanical allodynia and central sensitization that are associated with CRPS I and may provide a rationale for CRPS I treatment strategies using peroxynitrite decomposition catalysts.
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Copy and paste a formatted citation
Spandidos Publications style
Kwak K, Jung H, Park JM, Yeo J, Kim H, Lee HC, Byun SH, Kim J, Park S, Lim DG, Lim DG, et al: A peroxynitrite decomposition catalyst prevents mechanical allodynia and NMDA receptor activation in the hind-paw ischemia reperfusion injury rats. Exp Ther Med 7: 508-512, 2014.
APA
Kwak, K., Jung, H., Park, J.M., Yeo, J., Kim, H., Lee, H.C. ... Lim, D.G. (2014). A peroxynitrite decomposition catalyst prevents mechanical allodynia and NMDA receptor activation in the hind-paw ischemia reperfusion injury rats. Experimental and Therapeutic Medicine, 7, 508-512. https://doi.org/10.3892/etm.2013.1440
MLA
Kwak, K., Jung, H., Park, J. M., Yeo, J., Kim, H., Lee, H. C., Byun, S. H., Kim, J., Park, S., Lim, D. G."A peroxynitrite decomposition catalyst prevents mechanical allodynia and NMDA receptor activation in the hind-paw ischemia reperfusion injury rats". Experimental and Therapeutic Medicine 7.2 (2014): 508-512.
Chicago
Kwak, K., Jung, H., Park, J. M., Yeo, J., Kim, H., Lee, H. C., Byun, S. H., Kim, J., Park, S., Lim, D. G."A peroxynitrite decomposition catalyst prevents mechanical allodynia and NMDA receptor activation in the hind-paw ischemia reperfusion injury rats". Experimental and Therapeutic Medicine 7, no. 2 (2014): 508-512. https://doi.org/10.3892/etm.2013.1440
Copy and paste a formatted citation
x
Spandidos Publications style
Kwak K, Jung H, Park JM, Yeo J, Kim H, Lee HC, Byun SH, Kim J, Park S, Lim DG, Lim DG, et al: A peroxynitrite decomposition catalyst prevents mechanical allodynia and NMDA receptor activation in the hind-paw ischemia reperfusion injury rats. Exp Ther Med 7: 508-512, 2014.
APA
Kwak, K., Jung, H., Park, J.M., Yeo, J., Kim, H., Lee, H.C. ... Lim, D.G. (2014). A peroxynitrite decomposition catalyst prevents mechanical allodynia and NMDA receptor activation in the hind-paw ischemia reperfusion injury rats. Experimental and Therapeutic Medicine, 7, 508-512. https://doi.org/10.3892/etm.2013.1440
MLA
Kwak, K., Jung, H., Park, J. M., Yeo, J., Kim, H., Lee, H. C., Byun, S. H., Kim, J., Park, S., Lim, D. G."A peroxynitrite decomposition catalyst prevents mechanical allodynia and NMDA receptor activation in the hind-paw ischemia reperfusion injury rats". Experimental and Therapeutic Medicine 7.2 (2014): 508-512.
Chicago
Kwak, K., Jung, H., Park, J. M., Yeo, J., Kim, H., Lee, H. C., Byun, S. H., Kim, J., Park, S., Lim, D. G."A peroxynitrite decomposition catalyst prevents mechanical allodynia and NMDA receptor activation in the hind-paw ischemia reperfusion injury rats". Experimental and Therapeutic Medicine 7, no. 2 (2014): 508-512. https://doi.org/10.3892/etm.2013.1440
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