Increased expression of chymase in inflammatory polyps in elderly patients with functional bowel disorder

Rong,Jian-Ming; Ji,Hong-Zan; Wu,Xiao-Wei; Sun,Quan; Guo,Mei‑Xia; Xu,Xiao-Bing; Wang,Fang-Yu

doi:10.3892/etm.2013.1444

Increased expression of chymase in inflammatory polyps in elderly patients with functional bowel disorder

Authors:
- Jian-Ming Rong
- Hong-Zan Ji
- Xiao-Wei Wu
- Quan Sun
- Mei‑Xia Guo
- Xiao-Bing Xu
- Fang-Yu Wang
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Affiliations: Department of Geratology, No.454 Hospital of PLA, Nanjing, Jiangsu 210002, P.R. China, Department of Gastroenterology, Nanjing Jin Ling Hospital, Nanjing, Jiangsu 210002, P.R. China
Published online on: December 11, 2013 https://doi.org/10.3892/etm.2013.1444
Pages: 371-374

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Abstract

Chymase, a chymotrypsin‑like protease, is a non‑angiotensin‑converting enzyme (ACE) angiotensin II (Ang II)‑generating enzyme. The aim of the present study was to investigate whether chymase activity was increased in inflammatory polyps of elderly patients with functional bowel disorder (FBD). This study enrolled 45 elderly patients with FBD and 44 healthy control individuals. Expression of chymase in intestinal mucosa was assessed using fluorescence quantitative polymerase chain reaction and immunohistochemistry (IHC). IHC showed an increased number of chymase‑positive mast cells in inflammatory polyps than in healthy intestinal mucosa (P<0.05). Compared with healthy mucosa, expression of chymase at the mRNA and protein level was significantly higher in inflammatory polyps. The frequencies of the chymase GG genotype and the G allele type were higher in the intestinal mucosa of patients with FBD compared with healthy controls (66.67 versus 40.91%, 81.11 versus 63.63%, both P<0.05). The frequency of the G allele type in the intestinal mucosa of the C4 subgroup of FBD was higher than that in the control group. However, in other FBD subgroups, there was no difference between patients and controls. Based on the fact that enhanced chymase expression was observed in inflammatory polyps of elderly patients with FBD relative to those in healthy controls, it was concluded that chymase has a significant role in the pathogenesis of inflammatory polyps in elderly patients with FBD.

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1	Keating E, Lemos C, Monteiro R, Azevedo I and Martel F: The effect of a series of organic cations upon the plasmalemmal serotonin transporter, SERT. Life Sci. 76:103–119. 2004. View Article : Google Scholar : PubMed/NCBI
2	Mykletun A, Heradstveit O, Eriksen K, et al: Health anxiety and disability pension award: The HUSK Study. Psychosom Med. 71:353–360. 2009. View Article : Google Scholar : PubMed/NCBI
3	Ji HZ, Wu XW, Xu XB, et al: The correlation between functional bowel disorder in the elderly and hypertensive target organ damage. Chin J Prev Contr Chron Dis. 15:365–366. 2007.(In Chinese).
4	Corleto VD, Pagnini C, Cattaruzza MS, et al: Is proliferative colonic disease presentation changing? World J Gastroenterol. 18:6614–6619. 2012. View Article : Google Scholar : PubMed/NCBI
5	Wu X, Cokkinides V, Chen VW, et al: Associations of subsite-specific colorectal cancer incidence rates and stage of disease at diagnosis with county-level poverty, by race and sex. Cancer. 107:1121–1127. 2006. View Article : Google Scholar : PubMed/NCBI
6	Andoh A, Deguchi Y, Inatomi O, et al: Immunohistochemical study of chymase-positive mast cells in inflammatory bowel disease. Oncol Rep. 16:103–107. 2006.PubMed/NCBI
7	Daemen MJ, Lombardi DM, Bosman FT and Schwartz SM: Angiotensin II induces smooth muscle cell proliferation in the normal and injured rat arterial wall. Circ Res. 68:450–456. 1991. View Article : Google Scholar : PubMed/NCBI
8	Maltby S, Khazaie K and McNagny KM: Mast cells in tumor growth: angiogenesis, tissue remodelling and immune-modulation. Biochim Biophys Acta. 1796:19–26. 2009.PubMed/NCBI
9	He SH: Key role of mast cells and their major secretory products in inflammatory bowel disease. World J Gastroenterol. 10:309–318. 2004.PubMed/NCBI
10	Drossman DA: The functional gastrointestinal disorders and the Rome II process. Gut. 45(Suppl 2): II1–II5. 1999.PubMed/NCBI
11	Du W, Ji H, Cao S, et al: EpCAM: a potential antimetastatic target for gastric cancer. Dig Dis Sci. 55:2165–2171. 2010. View Article : Google Scholar : PubMed/NCBI
12	Drossman DA: The functional gastrointestinal disorders and the Rome III process. Gastroenterology. 130:1377–1390. 2006. View Article : Google Scholar : PubMed/NCBI
13	Takai S, Jin D, Sakaguchi M and Miyazaki M: Chymase-dependent angiotensin II formation in human vascular tissue. Circulation. 100:654–658. 1999. View Article : Google Scholar : PubMed/NCBI
14	Wu XW, Sun Q, Ji HZ, et al: Chymase genenic polymorphism of elderly patients with functional bowel disorders. Chin J Gerontol. 30:158–160. 2010.
15	Matsuzuka T, Miller K, Pickel L, Doi C, Ayuzawa R and Tamura M: The synergistic induction of cyclooxygenase-2 in lung fibroblasts by angiotensin II and pro-inflammatory cytokines. Mol Cell Biochem. 320:163–171. 2009. View Article : Google Scholar : PubMed/NCBI
16	Riaz AA, Wang Y, Schramm R, et al: Role of angiotensin II in ischemia/reperfusion-induced leukocyte-endothelium interactions in the colon. FASEB J. 18:881–883. 2004.PubMed/NCBI
17	Mohan M, Jaiswal BS and Kasture S: Effect of Solanum torvum on blood pressure and metabolic alterations in fructose hypertensive rats. Ethnopharmacol. 126:86–89. 2009. View Article : Google Scholar : PubMed/NCBI
18	Xu XB and Ji HZ: Anti-tumor activity of angiotensin-converting-enzyme inhibitors. J Med Postgraduates. 21:325–329. 2008.
19	Urata H: Pathological involvement of chymase-dependent angiotensin II formation in the development of cardiovascular disease. J Renin Angiotensin Aldosterone Syst. 1(2 Suppl): S35–S37. 2000. View Article : Google Scholar : PubMed/NCBI
20	Jin D, Takai S, Yamada M, et al: Impact of chymase inhibitor on cardiac function and survival after myocardial infarction. Cardiovasc Res. 60:413–420. 2003. View Article : Google Scholar : PubMed/NCBI
21	Kakimoto K, Takai S, Murano M, et al: Significance of chymase-dependent matrix metalloproteinase-9 activation on indomethacin-induced small intestinal damages in rats. J Pharmacol Exp Ther. 332:684–689. 2010. View Article : Google Scholar : PubMed/NCBI
22	Takai S, Jin D and Miyazaki M: New approaches to blockade of the renin-angiotensin-aldosterone system: chymase as an important target to prevent organ damage. J Pharmacol Sci. 113:301–309. 2010. View Article : Google Scholar : PubMed/NCBI