Tissue engineering is a promising method for the repair of spinal cord injuries (Review)

Ji,Wenchen; Hu,Shouye; Zhou,Jiao; Wang,Gang; Wang,Kunzheng; Zhang,Yuelin

doi:10.3892/etm.2013.1454

Tissue engineering is a promising method for the repair of spinal cord injuries (Review)

Authors:
- Wenchen Ji
- Shouye Hu
- Jiao Zhou
- Gang Wang
- Kunzheng Wang
- Yuelin Zhang
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Affiliations: Medical School of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Surgery, The Third Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an, Shaanxi 710068, P.R. China
Published online on: December 18, 2013 https://doi.org/10.3892/etm.2013.1454
Pages: 523-528

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Abstract

Spinal cord injury (SCI) may lead to a devastating and permanent loss of neurological function, which may place a great economic burden on the family of the patient and society. Methods for reducing the death of neuronal cells, inhibiting immune and inflammatory reactions, and promoting the growth of axons in order to build up synapses with the target cells are the focus of current research. Target cells are located in the damaged spinal cord which create a connect with the scaffold. As tissue engineering technology is developed for use in a variety of different areas, particularly the biomedical field, a clear understanding of the mechanisms of tissue engineering is important. This review establishes how this technology may be used in basic experiments with regard to SCI and considers its potential future clinical use.

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1	Findley PA, Banerjea R and Sambamoorthi U: Excess mortality associated with mental illness and substance use disorders among veteran clinic users with spinal cord injury. Disabil Rehabil. 33:1608–1615. 2011. View Article : Google Scholar : PubMed/NCBI
2	Wang M, Zhai P, Chen X, Schreyer DJ, Sun X and Cui F: Bioengineered scaffolds for spinal cord repair. Tissue Eng Part B Rev. 17:177–194. 2011. View Article : Google Scholar : PubMed/NCBI
3	Hulsebosch CE: Recent advances in pathophysiology and treatment of spinal cord injury. Adv Physiol Educ. 26:238–255. 2002.PubMed/NCBI
4	Baumgaertner W, Spitzbarth I and Beineke A: New knowledge on the pathology of spinal cord disease in dogs - views on therapy. Praktische Tierarzt. 93:794–796. 2012.
5	Wasner G: Spinal cord injury pain - from symptom to pathology. In: Proceedings of the 3rd International Congress on Neuropathic Pain (NeuPSIG); Medimond, Bologna. pp. 107–111. 2010
6	Shimizu H, Kakita A and Takahashi H: Spinal cord tau pathology in cervical spondylotic myelopathy. Acta Neuropathol. 115:185–192. 2008. View Article : Google Scholar : PubMed/NCBI
7	Norenberg MD, Smith J and Marcillo A: The pathology of human spinal cord injury: defining the problems. J Neurotrauma. 21:429–440. 2004. View Article : Google Scholar : PubMed/NCBI
8	Banerjea R, Findley PA, Smith B, Findley T and Sambamoorthi U: Co-occurring medical and mental illness and substance use disorders among veteran clinic users with spinal cord injury patients with complexities. Spinal Cord. 47:789–795. 2009. View Article : Google Scholar : PubMed/NCBI
9	May L, Day R and Warren S: Evaluation of patient education in spinal cord injury rehabilitation: knowledge, problem-solving and perceived importance. Disabil Rehabil. 28:405–413. 2006. View Article : Google Scholar : PubMed/NCBI
10	Cruz CD and Cruz F: Spinal cord injury and bladder dysfunction: new ideas about an old problem. ScientificWorldJournal. 11:214–234. 2011. View Article : Google Scholar : PubMed/NCBI
11	Qu WS, Tian DS, GUO ZB, et al: Inhibition of EGFR/MAPK signaling reduces microglial inflammatory response and the associated secondary damage in rats after spinal cord injury. J Neuroinflammation. 9:1782012. View Article : Google Scholar : PubMed/NCBI
12	Madigan NN, McMahon S, O’Brien T, Yaszemski MJ and Windebank AJ: Current tissue engineering and novel therapeutic approaches to axonal regeneration following spinal cord injury using polymer scaffolds. Respir Physiol Neurobiol. 169:183–199. 2009. View Article : Google Scholar
13	Hernandez J, Torres-Espín A and Navarro X: Adult stem cell transplants for spinal cord injury repair: current state in preclinical research. Curr Stem Cell Res Ther. 6:273–287. 2011. View Article : Google Scholar : PubMed/NCBI
14	He X, Fu W and Zheng J: Cell sources for trachea tissue engineering: past, present and future. Regen Med. 7:851–863. 2012.PubMed/NCBI
15	Malik A and Khan W: Stem cell therapy and tissue engineering applications for bone. Curr Stem Cell Res Ther. 8:183–184. 2013. View Article : Google Scholar : PubMed/NCBI
16	Khan WS and Malik A: Stem cell therapy and tissue engineering applications for cartilage regeneration. Curr Stem Cell Res Ther. 7:241–242. 2012. View Article : Google Scholar : PubMed/NCBI
17	Salehi M, Pasbakhsh P, Soleimani M, et al: Repair of spinal cord injury by co-transplantation of embryonic stem cell-derived motor neuron and olfactory ensheathing cell. Iran Biomed J. 13:125–135. 2009.
18	Wilcock AC, Swedlow JR and Storey KG: Mitotic spindle orientation distinguishes stem cell and terminal modes of neuron production in the early spinal cord. Development. 134:1943–1954. 2007. View Article : Google Scholar : PubMed/NCBI
19	Daadi MM, Grueter BA, Malenka RC, Redmond DE Jr and Steinberg GK: Dopaminergic neurons from midbrain-specified human embryonic stem cell-derived neural stem cells engrafted in a monkey model of Parkinson’s disease. PLoS One. 7:e411202012.PubMed/NCBI
20	Meamar R, Dehghani L and Karamali F: Toxicity effects of methamphetamine on embryonic stem cell-derived neuron. J Res Med Sci. 17:470–474. 2012.PubMed/NCBI
21	Tavares I: Human neural stem cell transplantation in spinal cord injury models: how far from clinical application? Stem Cell Res Ther. 4:612013. View Article : Google Scholar : PubMed/NCBI
22	Deda H, Inci MC, Kurekci AE, et al: Treatment of chronic spinal cord injured patients with autologous bone marrow-derived hematopoietic stem cell transplantation: 1-year follow-up. Cytotherapy. 10:565–574. 2008.PubMed/NCBI
23	Kim JW, Ha KY, Molon JN and Kim YH: Bone marrow-derived mesenchymal stem cell transplantation for chronic spinal cord injury in rats: comparative study between intralesional and intravenous transplantation. Spine (Phila Pa 1976). 38:E1065–E1074. 2013. View Article : Google Scholar : PubMed/NCBI
24	Alessandri G, Pagano S, Bez A, et al: Isolation and culture of human muscle-derived stem cells able to differentiate into myogenic and neurogenic cell lineages. Lancet. 364:1872–1883. 2004. View Article : Google Scholar : PubMed/NCBI
25	Lavasani M, Lu A, Thompson SD, Robbins PD, Huard J and Niedernhofer LJ: Isolation of muscle-derived stem/progenitor cells based on adhesion characteristics to collagen-coated surfaces. Methods Mol Biol. 976:53–65. 2013. View Article : Google Scholar : PubMed/NCBI
26	Wu X, Wang S, Chen B and An X: Muscle-derived stem cells: isolation, characterization, differentiation, and application in cell and gene therapy. Cell Tissue Res. 340:549–567. 2010. View Article : Google Scholar : PubMed/NCBI
27	Danisovic L, Varga I, Polák S, Bajciková B, Adamkov M and Vojtassák J: Biological and morphological characterization of in vitro expanded human muscle-derived stem cells. Tsitologiia. 53:482–487. 2011.PubMed/NCBI
28	Danisovic L, Varga I, Polák S, Ulicna M, Bohmer D and Vojtassák J: Morphology of in vitro expanded human muscle-derived stem cells. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 152:235–238. 2008. View Article : Google Scholar : PubMed/NCBI
29	Woo JC, Bae WJ, Kim SJ, et al: Transplantation of muscle-derived stem cells into the corpus cavernosum restores erectile function in a rat model of cavernous nerve injury. Korean J Urol. 52:359–363. 2011.PubMed/NCBI
30	Stulpinas A, Imbrasaité A and Kalvelyté AV: Daunorubicin induces cell death via activation of apoptotic signalling pathway and inactivation of survival pathway in muscle-derived stem cells. Cell Biol Toxicol. 28:103–114. 2012. View Article : Google Scholar : PubMed/NCBI
31	Shibuya M, Miura T, Fukagawa Y, et al: Tongue muscle-derived stem cells express connexin 43 and improve cardiac remodeling and survival after myocardial infarction in mice. Circ J. 74:1219–1226. 2010. View Article : Google Scholar : PubMed/NCBI
32	Kwon EB and Lee JY, Piao S, Kim IG, Ra JC and Lee JY: Comparison of human muscle-derived stem cells and human adipose-derived stem cells in neurogenic trans-differentiation. Korean J Urol. 52:852–857. 2011. View Article : Google Scholar : PubMed/NCBI
33	Kwon JS, Kim GH, Kim da Y, et al: Chitosan-based hydrogels to induce neuronal differentiation of rat muscle-derived stem cells. Int J Biol Macromol. 51:974–979. 2012. View Article : Google Scholar : PubMed/NCBI
34	Zuk PA: The adipose-derived stem cell: looking back and looking ahead. Mol Biol Cell. 21:1783–1787. 2010. View Article : Google Scholar : PubMed/NCBI
35	Wilson B, Liotta LA and Petricoin EF: Dynamic protein pathway activation mapping of adipose-derived stem cell differentiation implicates novel regulators of adipocyte differentiation. Mol Cell Proteomics. 12:2522–2535. 2013. View Article : Google Scholar
36	Su SJ, Chang KL, Su SH, Yeh YT, Shyu HW and Chen KM: Caffeine regulates osteogenic differentiation and mineralization of primary adipose-derived stem cells and a bone marrow stromal cell line. Int J Food Sci Nutr. 64:429–436. 2013. View Article : Google Scholar : PubMed/NCBI
37	Merceron C, Portron S, Masson M, et al: The effect of two- and three-dimensional cell culture on the chondrogenic potential of human adipose-derived mesenchymal stem cells after subcutaneous transplantation with an injectable hydrogel. Cell Transplant. 20:1575–1588. 2011. View Article : Google Scholar
38	Liqing Y, Jia G, Jiqing C, et al: Directed differentiation of motor neuron cell-like cells from human adipose-derived stem cells in vitro. Neuroreport. 22:370–373. 2011. View Article : Google Scholar : PubMed/NCBI
39	Valenzuela CD, Allori AC, Reformat DD, et al: Characterization of adipose-derived mesenchymal stem cell combinations for vascularized bone engineering. Tissue Eng Part A. 19:1373–1385. 2013. View Article : Google Scholar : PubMed/NCBI
40	Kleintjes WG: Treatment of basal cell carcinoma with autogenous growth factors and adipose-derived stem cells. Plast Reconstr Surg. 126:312e–313e. 2010. View Article : Google Scholar : PubMed/NCBI
41	Sterodimas A, de Faria J, Nicaretta B and Pitanguy I: Tissue engineering with adipose-derived stem cells (ADSCs): current and future applications. J Plast Reconstr Aesthet Surg. 63:1886–1892. 2010. View Article : Google Scholar : PubMed/NCBI
42	Oh JS, Park IS, Kim KN, Yoon do H, Kim SH and Ha Y: Transplantation of an adipose stem cell cluster in a spinal cord injury. Neuroreport. 23:277–282. 2012. View Article : Google Scholar : PubMed/NCBI
43	Barriga A, Medrano M, De-Juan J and Burgos J: Intravenous infusion of adult adipose tissue stem cells for repairing spinal cord ischaemic lesions. An experimental study on animals. Rev Esp Cir Ortop Traumatol. 57:89–94. 2013.(In Spanish).
44	Ferrero-Gutierrez A, Menendez-Menendez Y, Alvarez-Viejo M, Meana A and Otero J: New serum-derived albumin scaffold seeded with adipose-derived stem cells and olfactory ensheathing cells used to treat spinal cord injured rats. Histol Histopathol. 28:89–100. 2013.
45	Chung JY, Kim W, Im W, et al: Neuroprotective effects of adipose-derived stem cells against ischemic neuronal damage in the rabbit spinal cord. J Neurol Sci. 317:40–46. 2012. View Article : Google Scholar : PubMed/NCBI
46	Barry DS, Pakan JM, O’Keeffe GW and McDermott KW: The spatial and temporal arrangement of the radial glial scaffold suggests a role in axon tract formation in the developing spinal cord. J Anat. 222:203–213. 2013. View Article : Google Scholar : PubMed/NCBI
47	Roberts A, Li WC and Soffe SR: A functional scaffold of CNS neurons for the vertebrates: the developing Xenopus laevis spinal cord. Dev Neurobiol. 72:575–584. 2012. View Article : Google Scholar
48	Yoshii S, Ito S, Shima M, Taniguchi A and Akagi M: Functional restoration of rabbit spinal cord using collagen-filament scaffold. J Tissue Eng Regen Med. 3:19–25. 2009. View Article : Google Scholar : PubMed/NCBI
49	Ellis-Behnke RG and Schneider GE: Peptide amphiphiles and porous biodegradable scaffolds for tissue regeneration in the brain and spinal cord. Methods Mol Biol. 726:259–281. 2011. View Article : Google Scholar : PubMed/NCBI
50	Stokols S and Tuszynski MH: Freeze-dried agarose scaffolds with uniaxial channels stimulate and guide linear axonal growth following spinal cord injury. Biomaterials. 27:443–451. 2006. View Article : Google Scholar : PubMed/NCBI
51	Guo SZ, Ren XJ, Wu B and Jiang T: Preparation of the acellular scaffold of the spinal cord and the study of biocompatibility. Spinal Cord. 48:576–581. 2010. View Article : Google Scholar : PubMed/NCBI
52	Silva NA, Salgado AJ, Sousa RA, et al: Development and characterization of a novel hybrid tissue engineering-based scaffold for spinal cord injury repair. Tissue Eng Part A. 16:45–54. 2010. View Article : Google Scholar : PubMed/NCBI
53	Chung TW and Chang YL: Silk fibroin/chitosan-hyaluronic acid versus silk fibroin scaffolds for tissue engineering: promoting cell proliferations in vitro. J Mater Sci Mater Med. 21:1343–1351. 2010. View Article : Google Scholar : PubMed/NCBI
54	Hilmi AB, Halim AS, Hassan A, Lim CK, Noorsal K and Zainol I: In vitro characterization of a chitosan skin regenerating template as a scaffold for cells cultivation. Springerplus. 2:792013. View Article : Google Scholar
55	Guan L, Tian P, Ge H, et al: Chitosan-functionalized silk fibroin 3D scaffold for keratocyte culture. J Mol Histol. May 1–2013.(Epub ahead of print).
56	She Z, Liu W and Feng Q: Self-assembly model, hepatocytes attachment and inflammatory response for silk fibroin/chitosan scaffolds. Biomed Mater. 4:0450142009. View Article : Google Scholar : PubMed/NCBI
57	She Z, Jin C, Huang Z, Zhang B, Feng Q and Xu Y: Silk fibroin/chitosan scaffold: preparation, characterization, and culture with HepG2 cell. J Mater Sci Mater Med. 19:3545–3553. 2008. View Article : Google Scholar : PubMed/NCBI
58	Cigognini D, Satta A, Colleoni B, et al: Evaluation of early and late effects into the acute spinal cord injury of an injectable functionalized self-assembling scaffold. PLoS One. 6:e197822011. View Article : Google Scholar : PubMed/NCBI
59	Comolli N, Neuhuber B, Fischer I and Lowman A: In vitro analysis of PNIPAAm-PEG, a novel, injectable scaffold for spinal cord repair. Acta Biomater. 5:1046–1055. 2009. View Article : Google Scholar : PubMed/NCBI
60	Kubinová S, Horák D, Hejčl A, et al: SIKVAV-modified highly superporous PHEMA scaffolds with oriented pores for spinal cord injury repair. J Tissue Eng Regen Med. February 11–2013.(Epub ahead of print).
61	Kang KN, Lee JY, Kim da Y, et al: Regeneration of completely transected spinal cord using scaffold of poly(D,L-lactide-coglycolide)/small intestinal submucosa seeded with rat bone marrow stem cells. Tissue Eng Part A. 17:2143–2152. 2011. View Article : Google Scholar
62	Liu T, Houle JD, Xu J, Chan BP and Chew SY: Nanofibrous collagen nerve conduits for spinal cord repair. Tissue Eng Part A. 18:1057–1066. 2012. View Article : Google Scholar : PubMed/NCBI
63	Zhu Y, Wang A, Shen W, et al: Nanofibrous patches for spinal cord regeneration. Adv Funct Mater. 20:1433–1440. 2010. View Article : Google Scholar : PubMed/NCBI
64	Teng YD, Lavik EB, Qu X, et al: Functional recovery following traumatic spinal cord injury mediated by a unique polymer scaffold seeded with neural stem cells. Proc Natl Acad Sci USA. 99:3024–3029. 2002. View Article : Google Scholar : PubMed/NCBI
65	Du BL, Zeng CG, Zhang W, Quan DP, Ling EA and Zeng YS: A comparative study of gelatin sponge scaffolds and PLGA scaffolds transplanted to completely transected spinal cord of rat. J Biomed Mater Res A. June 15–2013.(Epub ahead of print).
66	McCall J, Weidner N and Blesch A: Neurotrophic factors in combinatorial approaches for spinal cord regeneration. Cell Tissue Res. 349:27–37. 2012. View Article : Google Scholar : PubMed/NCBI
67	de Leon RD: Could neurotrophins replace treadmill training as locomotor therapy following spinal cord injury? Focus on ‘neurotrophic factors promote and enhance locomotor recovery in untrained spinalized cats’. J Neurophysiol. 98:1845–1846. 2007.PubMed/NCBI
68	Sharma HS: Neurotrophic factors in combination: a possible new therapeutic strategy to influence pathophysiology of spinal cord injury and repair mechanisms. Curr Pharm Des. 13:1841–1874. 2007. View Article : Google Scholar : PubMed/NCBI
69	Sharma HS and Sharma A: Rodent spinal cord injury model and application of neurotrophic factors for neuroprotection. Methods Mol Biol. 846:393–415. 2012. View Article : Google Scholar : PubMed/NCBI
70	Berry A, Bindocci E and Alleva E: NGF, brain and behavioral plasticity. Neural Plast. 2012:7840402012.PubMed/NCBI
71	Allen SJ, Watson JJ, Shoemark DK, Barua NU and Patel NK: GDNF, NGF and BDNF as therapeutic options for neurodegeneration. Pharmacol Ther. 138:155–175. 2013. View Article : Google Scholar : PubMed/NCBI
72	Weishaupt N, Blesch A and Fouad K: BDNF: the career of a multifaceted neurotrophin in spinal cord injury. Exp Neurol. 238:254–264. 2012. View Article : Google Scholar : PubMed/NCBI
73	Weishaupt N, Li S, Di Pardo A, Sipione S and Fouad K: Synergistic effects of BDNF and rehabilitative training on recovery after cervical spinal cord injury. Behav Brain Res. 239:31–42. 2013. View Article : Google Scholar : PubMed/NCBI
74	Mantilla CB, Gransee HM, Zhan WZ and Sieck GC: Motoneuron BDNF/TrkB signaling enhances functional recovery after cervical spinal cord injury. Exp Neurol. 247:101–109. 2013. View Article : Google Scholar : PubMed/NCBI
75	Stokols S, Sakamoto J, Breckon C, Holt T, Weiss J and Tuszynski MH: Templated agarose scaffolds support linear axonal regeneration. Tissue Eng. 12:2777–2787. 2006. View Article : Google Scholar : PubMed/NCBI
76	Shang AJ, Hong SQ, Xu Q, et al: NT-3-secreting human umbilical cord mesenchymal stromal cell transplantation for the treatment of acute spinal cord injury in rats. Brain Res. 1391:102–113. 2011. View Article : Google Scholar : PubMed/NCBI
77	Shumsky JS, Tobias CA, Tumolo M, Long WD, Giszter SF and Murray M: Delayed transplantation of fibroblasts genetically modified to secrete BDNF and NT-3 into a spinal cord injury site is associated with limited recovery of function. Exp Neurol. 184:114–130. 2003. View Article : Google Scholar : PubMed/NCBI
78	Chen Q, Smith GM and Shine HD: Immune activation is required for NT-3-induced axonal plasticity in chronic spinal cord injury. Exp Neurol. 209:497–509. 2008. View Article : Google Scholar : PubMed/NCBI
79	Guo JS, Zeng YS, Li HB, et al: Cotransplant of neural stem cells and NT-3 gene modified Schwann cells promote the recovery of transected spinal cord injury. Spinal Cord. 45:15–24. 2007. View Article : Google Scholar : PubMed/NCBI
80	Wang X, Li Y, Gao Y, et al: Combined use of spinal cord-mimicking partition type scaffold architecture and neurotrophin-3 for surgical repair of completely transected spinal cord in rats. J Biomater Sci Polym Ed. 24:927–939. 2013. View Article : Google Scholar : PubMed/NCBI
81	Hara T, Fukumitsu H, Soumiya H, Furukawa Y and Furukawa S: Injury-induced accumulation of glial cell line-derived neurotrophic factor in the rostral part of the injured rat spinal cord. Int J Mol Sci. 13:13484–13500. 2012. View Article : Google Scholar : PubMed/NCBI
82	Lin S, Wang Y, Zhang C and Xu J: Modification of the neurotrophin-3 gene promotes cholinergic neuronal differentiation and survival of neural stem cells derived from rat embryonic spinal cord in vitro and in vivo. J Int Med Res. 40:1449–1458. 2012. View Article : Google Scholar : PubMed/NCBI
83	Yao L, Yao S, Daly W, Hendry W, Windebank A and Pandit A: Non-viral gene therapy for spinal cord regeneration. Drug Discov Today. 17:998–1005. 2012. View Article : Google Scholar : PubMed/NCBI
84	Bo X, Wu D, Yeh J and Zhang Y: Gene therapy approaches for neuroprotection and axonal regeneration after spinal cord and spinal root injury. Curr Gene Ther. 11:101–115. 2011. View Article : Google Scholar : PubMed/NCBI
85	Blesch A, Fischer I and Tuszynski MH: Gene therapy, neurotrophic factors and spinal cord regeneration. Handb Clin Neurol. 109:563–574. 2012. View Article : Google Scholar : PubMed/NCBI
86	Foust KD, Flotte TR, Reier PJ and Mandel RJ: Recombinant adeno-associated virus-mediated global anterograde delivery of glial cell line-derived neurotrophic factor to the spinal cord: comparison of rubrospinal and corticospinal tracts in the rat. Hum Gene Ther. 19:71–82. 2008. View Article : Google Scholar
87	Koda M, Hashimoto M, Murakami M, et al: Adenovirus vector-mediated in vivo gene transfer of brain-derived neurotrophic factor (BDNF) promotes rubrospinal axonal regeneration and functional recovery after complete transection of the adult rat spinal cord. J Neurotrauma. 21:329–337. 2004. View Article : Google Scholar
88	Blits B, Kitay BM, Farahvar A, Caperton CV, Dietrich WD and Bunge MB: Lentiviral vector-mediated transduction of neural progenitor cells before implantation into injured spinal cord and brain to detect their migration, deliver neurotrophic factors and repair tissue. Restor Neurol Neurosci. 23:313–324. 2005.
89	Morizono K and Chen IS: Targeted gene delivery by intravenous injection of retroviral vectors. Cell Cycle. 4:854–856. 2005. View Article : Google Scholar : PubMed/NCBI