Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
2014-March Volume 7 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
2014-March Volume 7 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

The evidence and the possible significance of autophagy in degeneration model of human cervical end-plate cartilage

  • Authors:
    • Hongguang Xu
    • Shouliang Xiong
    • Hong Wang
    • Min Zhang
    • Yunfei Yu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Yijishan Hospital, Wannan Medical College, Wuhu, Anhui 241001, P.R. China
  • Pages: 537-542
    |
    Published online on: December 30, 2013
       https://doi.org/10.3892/etm.2013.1465
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of this study was to observe autophagy in chondrocytes from degenerative human cervical vertebral end‑plates and to investigate the significance of variations in autophagy in the degeneration of cervical vertebral end‑plate chondrocytes. Cartilage end‑plates were obtained from 48 inpatients admitted to hospital between February 2011 and August 2012. The patients were divided into the control group (n=17) with cervical vertebral fracture or dislocation and the cervical spondylosis group (n=31) with cervical spondylotic myelopathy. End-plate chondrocytes were isolated via enzyme digestion and then cultured in vitro. The cells were stained with toluidine blue and hematoxylin-eosin (H&E). A laser scanning confocal microscope and monodansylcadaverine (MDC) were used to reveal autophagy in the end-plate chondrocytes. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect mRNA expression of type II collagen and aggrecan. Western blotting was conducted to detect LC3 proteins. The chondrocytes isolated from the degenerative human cervical end-plates were cultured successfully in vitro. The morphology of the cells from the cervical spondylosis group tended to exhibit changes in spindle morphology compared with the control group. Autophagic bodies were stained with MDC. LC3 proteins were visible in the intracellular and perinuclear regions under the laser scanning confocal microscope. The mRNA expression levels (relative to those of β-actin) of aggrecan (0.715±0.194) and type II collagen (0.628±0.254) in the cervical spondylosis group were markedly decreased compared with those in the control group (0.913±0.254 and 0.845±0.186, respectively; both P<0.05). The LC3-II/LC3-I ratio was observed to be significantly reduced in the cervical spondylosis group by Western blot analysis. Autophagy has an important role in human cervical disc degeneration. The regulation of autophagy may prevent disc degeneration in cartilage end-plate cells.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Roberts S and Johnson WE: Analysis of aging and degeneration of the human intervertebral disc. Spine (Phila Pa 1976). 24:500–501. 1999.PubMed/NCBI

2 

Jing L-B, Zhang X-L, Xu H-Z, et al: Protective effect of autophagy on nucleus pulposus cells under starvation. Chin J Pathophysiol. 28:1302–1307. 2012.(In Chinese).

3 

Haschtmann D, Stoyanov JV, Gédet P and Ferguson SJ: Vertebral endplate trauma induces disc cell apoptosis and promotes organ degeneration in vitro. Eur Spine J. 17:289–299. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Reggiori F and Klionsky DJ: Autophagy in the eukaryotic cell. Eukaryot cell. 1:11–21. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Chhangani D and Mishra A: Mahogunin ring finger-1 (MGRN1) suppresses chaperone-associated misfolded protein aggregation and toxicity. Sci Rep. 3:19722013. View Article : Google Scholar : PubMed/NCBI

6 

Liu H, Cao W, Li Y, Feng M, Wu X, Yu K and Liao M: Subgroup J avian leukosis virus infection inhibits autophagy in DF-1 cells. Virol J. 10:1962013. View Article : Google Scholar : PubMed/NCBI

7 

Miller G: The spine. Berquist TH: MRI of the Musculoskeletal System. 2nd edition. Raven Press; New York, NY: pp. 238–240. 1990

8 

Thompson JP, Pearce RH, Schechter MT, Adams ME, Tsang IK and Bishop PB: Preliminary evaluation of a scheme for grading the gross morphology of the human intervertehral disc. Spine (Phila Pa 1976). 15:411–415. 1990. View Article : Google Scholar : PubMed/NCBI

9 

Xu HG, Peng HX, Cheng JF and Lü K: Establishment and significance of an in vitro model of degeneration of human cervical endplate chondrocytes. Zhonghua Yi Xue Za Zhi. 91:2912–2916. 2011.(In Chinese).

10 

Le Maitre CL, Freemont AJ and Hoyland JA: The role of interleukin-1 in the pathogenesis of human intervertebral disc degeneration. Arthritis Res Ther. 7:R732–R745. 2005.

11 

Biederbick A, Kern HF and Elsässer HP: Monodansylcadaverine (MDC) is a specific in vivo marker for autophagic vacuoles. Eur J cell Biol. 66:3–14. 1995.PubMed/NCBI

12 

Shen C, Yan J, Jiang LS and Dai LY: Autophagy in rat annulus fibrosus cells: evidence and possible implications. Arthritis Res Ther. 13:R1322011. View Article : Google Scholar : PubMed/NCBI

13 

Liang QQ, Cui XJ, Xi ZJ, et al: Prolonged upright posture induces degenerative changes in intervertebral discs of rat cervical spine. Spine (Phila Pa 1976). 36:E14–E19. 2011.PubMed/NCBI

14 

Sowa G, Vadalà G, Studer R, et al: Characterization of intervertebral disc aging: longitudinal analysis of a rabbit model by magnetic resonance imaging, histology, and gene expression. Spine (Phila Pa 1976). 33:1821–1828. 2008. View Article : Google Scholar

15 

Shin BH, Lim Y, Oh HJ, et al: Pharmacological activation of Sirt1 ameliorates polyglutamine-induced toxicity through the regulation of autophagy. PLoS One. 8:e649532013. View Article : Google Scholar : PubMed/NCBI

16 

Caramés B, Taniguchi N, Otsuki S, Blanco FJ and Lotz M: Autophagy is a protective mechanism in normal cartilage, and its aging-related loss is linked with cell death and osteoarthritis. Arthritis Rheum. 62:791–801. 2010.PubMed/NCBI

17 

Sun K, Xie X, Liu Y, et al: Autophagy lessens ischemic liver injury by reducing oxidative damage. Cell Biosci. 3:262013. View Article : Google Scholar : PubMed/NCBI

18 

Zhu X, Wu L, Qiao H, et al: Autophagy stimulates apoptosis in HER2-overexpressing breast cancers treated by lapatinib. J Cell Biochem. 114:2643–2653. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Bachar-Wikstrom E, Wikstrom JD, Ariav Y, Tirosh B, Kaiser N, Cerasi E and Leibowitz G: Stimulation of autophagy improves endoplasmic reticulum stress-induced diabetes. Diabetes. 62:1227–1237. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Caramés B, Hasegawa A, Taniguchi N, Miyaki S, Blanco FJ and Lotz M: Autophagy activation by rapamycin reduces severity of experimental osteoarthritis. Ann Rheum Dis. 71:575–581. 2012.PubMed/NCBI

21 

Mori K: Tripartite management of unfolded proteins in the endoplasmic reticulum. Cell. 101:451–454. 2000. View Article : Google Scholar : PubMed/NCBI

22 

Kim HS, Montana V, Jang HJ, Parpura V and Kim JA: Epigallocatechin gallate (EGCG) stimulates autophagy in vascular endothelial cells: A potential role for reducing lipid accumulation. J Biol Chem. 288:22693–22705. 2013. View Article : Google Scholar

23 

Sanjuan MA, Dillon CP, Tait SW, et al: Toll-like receptor signalling in macrophages links the autophagy pathway to phagocytosis. Nature. 450:1253–1257. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Ye W, Xu K, Huang D, Liang A, Peng Y, Zhu W and Li C: Age-related increases of macroautophagy and chaperone-mediated autophagy in rat nucleus pulposus. Connect Tissue Res. 52:472–478. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Zhu W-R, Ye W, Xu K, Lou Z-K, Ren J-K, Chen J-H and Li C-H: Starvation-induced changes of LC-3 and Beclin-1 expression in annulus fibrosus cells. Chin J Exp Sur. 28:983–985. 2011.(In Chinese).

26 

Ye W, Chu Z, Xu K, et al: Expression and significance of Beclin-1 and microtubule-associated protein 1 light chain 3 in nucleus pulpous of rats during the aging process. Chin J Exp Surg. 27:643–645. 2010.

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Xu H, Xiong S, Wang H, Zhang M and Yu Y: The evidence and the possible significance of autophagy in degeneration model of human cervical end-plate cartilage. Exp Ther Med 7: 537-542, 2014.
APA
Xu, H., Xiong, S., Wang, H., Zhang, M., & Yu, Y. (2014). The evidence and the possible significance of autophagy in degeneration model of human cervical end-plate cartilage. Experimental and Therapeutic Medicine, 7, 537-542. https://doi.org/10.3892/etm.2013.1465
MLA
Xu, H., Xiong, S., Wang, H., Zhang, M., Yu, Y."The evidence and the possible significance of autophagy in degeneration model of human cervical end-plate cartilage". Experimental and Therapeutic Medicine 7.3 (2014): 537-542.
Chicago
Xu, H., Xiong, S., Wang, H., Zhang, M., Yu, Y."The evidence and the possible significance of autophagy in degeneration model of human cervical end-plate cartilage". Experimental and Therapeutic Medicine 7, no. 3 (2014): 537-542. https://doi.org/10.3892/etm.2013.1465
Copy and paste a formatted citation
x
Spandidos Publications style
Xu H, Xiong S, Wang H, Zhang M and Yu Y: The evidence and the possible significance of autophagy in degeneration model of human cervical end-plate cartilage. Exp Ther Med 7: 537-542, 2014.
APA
Xu, H., Xiong, S., Wang, H., Zhang, M., & Yu, Y. (2014). The evidence and the possible significance of autophagy in degeneration model of human cervical end-plate cartilage. Experimental and Therapeutic Medicine, 7, 537-542. https://doi.org/10.3892/etm.2013.1465
MLA
Xu, H., Xiong, S., Wang, H., Zhang, M., Yu, Y."The evidence and the possible significance of autophagy in degeneration model of human cervical end-plate cartilage". Experimental and Therapeutic Medicine 7.3 (2014): 537-542.
Chicago
Xu, H., Xiong, S., Wang, H., Zhang, M., Yu, Y."The evidence and the possible significance of autophagy in degeneration model of human cervical end-plate cartilage". Experimental and Therapeutic Medicine 7, no. 3 (2014): 537-542. https://doi.org/10.3892/etm.2013.1465
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team