Protective effects of Quercus salicina on alloxan‑induced oxidative stress in HIT‑T15 pancreatic β cells

  • Authors:
    • Jia‑Le Song
    • Xin Zhao
    • Qiang Wang
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  • Published online on: January 7, 2013     https://doi.org/10.3892/etm.2013.885
  • Pages: 947-951
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Abstract

The present study was designed to investigate the protective effect of hot water extracts from Quercus salicina leaves (QSWE) on alloxan‑induced oxidative stress in HIT‑T15 Syrian hamster pancreatic insulinoma cells. The HIT‑T15 cells were treated with alloxan (1 mM) for 1 h and then co‑incubated with the QSWE for 24 h. Alloxan significantly decreased the viability of the HIT-T15 cells (P<0.05). QSWE did not exhibit significantly cytotoxic effects and increased the viability of the HIT‑T15 cells in a concentration‑dependent manner. To further investigate the protective effects of QSWE on alloxan‑induced oxidative stress in HIT‑T15 cells, the cellular levels of reactive oxygen species (ROS), lipid peroxidation and endogenous antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH‑px), were analyzed. QSWE decreased the intracellular levels of ROS and lipid peroxidation and increased the activity of antioxidant enzymes. These results suggest that QSWE exerted cytoprotective activity against alloxan‑induced oxidative stress in HIT‑T15 cells through the inhibition of lipid peroxidation, reduction of ROS levels and stimulation of antioxidant enzyme activity. In addition, QSWE also increased the insulin secretion activity of the alloxan‑treated HIT‑T15 cells.
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March 2013
Volume 5 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Song JL, Zhao X and Wang Q: Protective effects of Quercus salicina on alloxan‑induced oxidative stress in HIT‑T15 pancreatic β cells. Exp Ther Med 5: 947-951, 2013.
APA
Song, J., Zhao, X., & Wang, Q. (2013). Protective effects of Quercus salicina on alloxan‑induced oxidative stress in HIT‑T15 pancreatic β cells. Experimental and Therapeutic Medicine, 5, 947-951. https://doi.org/10.3892/etm.2013.885
MLA
Song, J., Zhao, X., Wang, Q."Protective effects of Quercus salicina on alloxan‑induced oxidative stress in HIT‑T15 pancreatic β cells". Experimental and Therapeutic Medicine 5.3 (2013): 947-951.
Chicago
Song, J., Zhao, X., Wang, Q."Protective effects of Quercus salicina on alloxan‑induced oxidative stress in HIT‑T15 pancreatic β cells". Experimental and Therapeutic Medicine 5, no. 3 (2013): 947-951. https://doi.org/10.3892/etm.2013.885