Effects of adipose stem cell-conditioned medium on the migration of vascular endothelial cells, fibroblasts and keratinocytes Retraction in /10.3892/etm.2016.3709

Hu,Li; Zhao,Jiajia; Liu,Jiarong; Gong,Niya; Chen,Lili

doi:10.3892/etm.2013.887

Effects of adipose stem cell-conditioned medium on the migration of vascular endothelial cells, fibroblasts and keratinocytes

Retraction in: /10.3892/etm.2016.3709

Authors:
- Li Hu
- Jiajia Zhao
- Jiarong Liu
- Niya Gong
- Lili Chen
View Affiliations

Affiliations: Department of Stomatology, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, PR China
Published online on: January 7, 2013 https://doi.org/10.3892/etm.2013.887
Pages: 701-706

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Adipose stem cell‑conditioned medium (ASC‑CM) has been successfully used to treat multiple types of tissue and organ defects, including skin wounds both in vitro and in vivo. However, the mechanisms through which ASC-CM promotes wound healing remain unclear. We hypothesized that the wound healing effect of ASC‑CM is mediated in part by the promotion of the migration of vascular endothelial cells, fibroblasts and keratinocytes, the three cell types essential for wound healing. We reported that ASC‑CM stimulated the migration of these cells sequentially, and endothelial cells were the first cell type to respond to ASC‑CM stimulation (4 h), followed by fibroblasts (12 h) and then keratinocytes (24 h). We also determined the optimal concentration of ASC‑CM in stimulating these cells (50% dilution) in addition to the optimal time to intervene in order to maximize the wound healing activity of ASC‑CM. Our data suggest an important role for ASC‑CM in wound healing, possibly through the synthetic action of multiple adipose stem cell‑derived cytokines that in turn promote cell migration. Thus, ASC‑CM appears to have significant potential in wound healing applications.

View Figures

View References

1.	Zuk PA, Zhu M, Mizuno H, et al: Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 7:211–228. 2001. View Article : Google Scholar : PubMed/NCBI
2.	Bunnell BA, Flaat M, Gagliardi C, Patel B and Ripoll C: Adipose-derived stem cells: isolation, expansion and differentiation. Methods. 45:115–120. 2008. View Article : Google Scholar : PubMed/NCBI
3.	Hong SJ, Traktuev DO and March KL: Therapeutic potential of adipose-derived stem cells in vascular growth and tissue repair. Curr Opin Organ Transplant. 15:86–91. 2010. View Article : Google Scholar : PubMed/NCBI
4.	Fernyhough ME, Hausman GJ, Guan LL, Okine E, Moore SS and Dodson MV: Mature adipocytes may be a source of stem cells for tissue engineering. Biochem Biophys Res Commun. 368:455–457. 2008.PubMed/NCBI
5.	Sterodimas A, de Faria J, Nicaretta B and Pitanguy I: Tissue engineering with adipose-derived stem cells (ADSCs): current and future applications. J Plast Reconstr Aesthet Surg. 63:1886–1892. 2010. View Article : Google Scholar : PubMed/NCBI
6.	Liu J, Mao JJ and Chen L: Epithelial-mesenchymal interactions as a working concept for oral mucosa regeneration. Tissue Eng Part B Rev. 17:25–31. 2011. View Article : Google Scholar : PubMed/NCBI
7.	Kim WS, Park BS, Sung JH, Yang JM, Park SB, Kwak SJ and Park JS: Wound healing effect of adipose-derived stem cells: a critical role of secretory factors on human dermal fibroblasts. J Dermatol Sci. 48:15–24. 2007. View Article : Google Scholar : PubMed/NCBI
8.	Kondo T and Ishida Y: Molecular pathology of wound healing. Forensic Sci Int. 203:93–98. 2010. View Article : Google Scholar : PubMed/NCBI
9.	Fu X, Fang L, Li H, Li X, Cheng B and Sheng Z: Adipose tissue extract enhances skin wound healing. Wound Repair Regen. 15:540–548. 2007. View Article : Google Scholar : PubMed/NCBI
10.	Baraniak PR and McDevitt TC: Stem cell paracrine actions and tissue regeneration. Regen Med. 5:121–143. 2010. View Article : Google Scholar : PubMed/NCBI
11.	Casteilla L, Planat-Benard V, Laharrague P and Cousin B: Adipose-derived stromal cells: their identity and uses in clinical trials, an update. World J Stem Cells. 3:25–33. 2011. View Article : Google Scholar : PubMed/NCBI
12.	Yuan Z, Nie H, Wang S, et al: Biomaterial selection for tooth regeneration. Tissue Eng Part B Rev. 17:373–388. 2011. View Article : Google Scholar : PubMed/NCBI
13.	Häkkinen L, Koivisto L and Larjava H: An improved method for culture of epidermal keratinocytes from newborn mouse skin. Methods Cell Sci. 23:189–196. 2001.PubMed/NCBI
14.	Li S, Huang NF and Hsu S: Mechanotransduction in endothelial cell migration. J Cell Biochem. 96:1110–1126. 2005. View Article : Google Scholar
15.	Liebl J, Weitensteiner SB, Vereb G, Takács L, Fürst R, Vollmar AM and Zahler S: Cyclin-dependent kinase 5 regulates endothelial cell migration and angiogenesis. J Biol Chem. 285:35932–35943. 2010. View Article : Google Scholar : PubMed/NCBI
16.	Trochon V, Mabilat C, Bertrand P, et al: Evidence of involvement of CD44 in endothelial cell proliferation, migration and angiogenesis in vitro. Int J Cancer. 66:664–668. 1996. View Article : Google Scholar : PubMed/NCBI
17.	Cao G, O’Brien CD, Zhou Z, et al: Involvement of human PECAM-1 in angiogenesis and in vitro endothelial cell migration. Am J Physiol Cell Physiol. 282:C1181–C1190. 2002. View Article : Google Scholar : PubMed/NCBI
18.	Kanazawa S, Fujiwara T, Matsuzaki S, et al: bFGF regulates PI3-kinase-Rac1-JNK pathway and promotes fibroblast migration in wound healing. PLoS One. 5:e122282010. View Article : Google Scholar : PubMed/NCBI
19.	Maheshwari G, Wells A, Griffith LG and Lauffenburger DA: Biophysical integration of effects of epidermal growth factor and fibronectin on fibroblast migration. Biophys J. 76:2814–2823. 1999. View Article : Google Scholar : PubMed/NCBI
20.	Bae JS, Lee SH, Kim JE, et al: Betaig-h3 supports keratinocyte adhesion, migration, and proliferation through alpha3beta1 integrin. Biochem Biophys Res Commun. 294:940–948. 2002. View Article : Google Scholar : PubMed/NCBI
21.	Walter MN, Wright KT, Fuller HR, MacNeil S and Johnson WE: Mesenchymal stem cell-conditioned medium accelerates skin wound healing: an in vitro study of fibroblast and keratinocyte scratch assays. Exp Cell Res. 316:1271–1281. 2010. View Article : Google Scholar : PubMed/NCBI