Effects of adipose stem cell-conditioned medium on the migration of vascular endothelial cells, fibroblasts and keratinocytes Retraction in /10.3892/etm.2016.3709

Hu,Li; Zhao,Jiajia; Liu,Jiarong; Gong,Niya; Chen,Lili

doi:10.3892/etm.2013.887

March 2013 Volume 5 Issue 3

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March 2013 Volume 5 Issue 3

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Article

Effects of adipose stem cell-conditioned medium on the migration of vascular endothelial cells, fibroblasts and keratinocytes

Retraction in: /10.3892/etm.2016.3709

Authors:
- Li Hu
- Jiajia Zhao
- Jiarong Liu
- Niya Gong
- Lili Chen
View Affiliations / Copyright

Affiliations: Department of Stomatology, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, PR China
Pages: 701-706
|
Published online on: January 7, 2013

https://doi.org/10.3892/etm.2013.887
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Abstract

Adipose stem cell‑conditioned medium (ASC‑CM) has been successfully used to treat multiple types of tissue and organ defects, including skin wounds both in vitro and in vivo. However, the mechanisms through which ASC-CM promotes wound healing remain unclear. We hypothesized that the wound healing effect of ASC‑CM is mediated in part by the promotion of the migration of vascular endothelial cells, fibroblasts and keratinocytes, the three cell types essential for wound healing. We reported that ASC‑CM stimulated the migration of these cells sequentially, and endothelial cells were the first cell type to respond to ASC‑CM stimulation (4 h), followed by fibroblasts (12 h) and then keratinocytes (24 h). We also determined the optimal concentration of ASC‑CM in stimulating these cells (50% dilution) in addition to the optimal time to intervene in order to maximize the wound healing activity of ASC‑CM. Our data suggest an important role for ASC‑CM in wound healing, possibly through the synthetic action of multiple adipose stem cell‑derived cytokines that in turn promote cell migration. Thus, ASC‑CM appears to have significant potential in wound healing applications.

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