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Article

Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro

  • Authors:
    • Xiao-Man Xu
    • Yi Zhang
    • Dan Qu
    • Hong-Bo Liu
    • Xiu Gu
    • Guang-Yu Jiao
    • Li Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China, Department of Geriatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China
  • Pages: 707-710
    |
    Published online on: January 8, 2013
       https://doi.org/10.3892/etm.2013.889
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Abstract

Drug combination therapies are common practice in the treatment of cancer. Cisplatin is the most active chemotherapeutic agent for lung cancer treatment. Osthole is a natural compound extracted from a number of medicinal plants. To determine whether osthole enhances the anticancer effect of cisplatin in human lung cancer, we treated NCI‑H460 cells with osthole alone or in combination with cisplatin and evaluated cell growth and apoptosis using 3‑(4,5‑dimethyl thiazol‑2yl)‑2,5‑diphenyltetrazolium bromide (MTT) assay, flow cytometry and fluorescence microscopy. The results showed that, in comparison with single agent treatment, the combination of osthole and cisplatin resulted in greater efficacy in growth inhibition and apoptosis induction. Western blot analysis revealed that the combination effect of osthole and cisplatin was due to regulation of the Bcl‑2 family proteins. Findings of this investigation suggested that osthole combined with cisplatin is a potential clinical chemotherapeutic approach in human lung cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Xu X, Zhang Y, Qu D, Liu H, Gu X, Jiao G and Zhao L: Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro. Exp Ther Med 5: 707-710, 2013.
APA
Xu, X., Zhang, Y., Qu, D., Liu, H., Gu, X., Jiao, G., & Zhao, L. (2013). Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro. Experimental and Therapeutic Medicine, 5, 707-710. https://doi.org/10.3892/etm.2013.889
MLA
Xu, X., Zhang, Y., Qu, D., Liu, H., Gu, X., Jiao, G., Zhao, L."Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro". Experimental and Therapeutic Medicine 5.3 (2013): 707-710.
Chicago
Xu, X., Zhang, Y., Qu, D., Liu, H., Gu, X., Jiao, G., Zhao, L."Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro". Experimental and Therapeutic Medicine 5, no. 3 (2013): 707-710. https://doi.org/10.3892/etm.2013.889
Copy and paste a formatted citation
x
Spandidos Publications style
Xu X, Zhang Y, Qu D, Liu H, Gu X, Jiao G and Zhao L: Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro. Exp Ther Med 5: 707-710, 2013.
APA
Xu, X., Zhang, Y., Qu, D., Liu, H., Gu, X., Jiao, G., & Zhao, L. (2013). Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro. Experimental and Therapeutic Medicine, 5, 707-710. https://doi.org/10.3892/etm.2013.889
MLA
Xu, X., Zhang, Y., Qu, D., Liu, H., Gu, X., Jiao, G., Zhao, L."Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro". Experimental and Therapeutic Medicine 5.3 (2013): 707-710.
Chicago
Xu, X., Zhang, Y., Qu, D., Liu, H., Gu, X., Jiao, G., Zhao, L."Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro". Experimental and Therapeutic Medicine 5, no. 3 (2013): 707-710. https://doi.org/10.3892/etm.2013.889
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