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Expression of a tumor-associated gene, LASS2, in the human bladder carcinoma cell lines BIU-87, T24, EJ and EJ-M3

  • Authors:
    • Qinghua Zhao
    • Haifeng Wang
    • Mingying Yang
    • Delin  Yang
    • Yigang Zuo
    • Jiansong Wang

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  • Published online on: January 11, 2013     https://doi.org/10.3892/etm.2013.892
  • Pages: 942-946
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Abstract

Homo sapiens longevity assurance homolog 2 of yeast LAG1 (LASS2), a metastasis suppressor gene of human cancer, is the most abundantly expressed member of the ceramide synthase gene family. Expression of LASS2 has been reported in carcinomas of the prostate, liver and breast. However, there has been no report on the expression of LASS2 in human bladder cancer cell lines. In order to investigate the expression and potential role of this new tumor metastasis supressor gene in human bladder cancer, we compared the proliferation, metastasis and invasion among the BIU-87, T24, EJ and EJ-M3 human bladder cancer cell lines. The mRNA expression levels of the LASS2 gene were examined using real-time quantitative PCR (qPCR). The expression levels of LASS1 and LASS3 mRNA were used as references. The protein expression level of the LASS2 gene was detected using western blotting. The most aggressive of these four human cancer cell lines was observed to be EJ-M3. The expression of LASS2 mRNA was significantly correlated with diverse proliferation, metastasis and invasion. The expression levels of LASS1 and LASS3 mRNA were not correlated with these parameters. At the protein level, we observed that the more aggressive the cancer cell line, the lower the LASS2 protein expression level. Therefore, LASS2 expression may be correlated with the development and progression of human bladder cancer and may be a prognostic indicator for this cancer.
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March 2013
Volume 5 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhao Q, Wang H, Yang M, Yang D, Zuo Y and Wang J: Expression of a tumor-associated gene, LASS2, in the human bladder carcinoma cell lines BIU-87, T24, EJ and EJ-M3. Exp Ther Med 5: 942-946, 2013
APA
Zhao, Q., Wang, H., Yang, M., Yang, D., Zuo, Y., & Wang, J. (2013). Expression of a tumor-associated gene, LASS2, in the human bladder carcinoma cell lines BIU-87, T24, EJ and EJ-M3. Experimental and Therapeutic Medicine, 5, 942-946. https://doi.org/10.3892/etm.2013.892
MLA
Zhao, Q., Wang, H., Yang, M., Yang, D., Zuo, Y., Wang, J."Expression of a tumor-associated gene, LASS2, in the human bladder carcinoma cell lines BIU-87, T24, EJ and EJ-M3". Experimental and Therapeutic Medicine 5.3 (2013): 942-946.
Chicago
Zhao, Q., Wang, H., Yang, M., Yang, D., Zuo, Y., Wang, J."Expression of a tumor-associated gene, LASS2, in the human bladder carcinoma cell lines BIU-87, T24, EJ and EJ-M3". Experimental and Therapeutic Medicine 5, no. 3 (2013): 942-946. https://doi.org/10.3892/etm.2013.892