Propyl gallate inhibits TPA‑induced inflammation via the nuclear factor‑κB pathway in human THP‑1 monocytes

Hsu,Hung‑Chih; Lin,Wan‑Chen; Chang,Pey‑Jium; Hong,Chang‑Zern; Chen,Ching‑Hsein

doi:10.3892/etm.2013.896

Propyl gallate inhibits TPA‑induced inflammation via the nuclear factor‑κB pathway in human THP‑1 monocytes

Authors:
- Hung‑Chih Hsu
- Wan‑Chen Lin
- Pey‑Jium Chang
- Chang‑Zern Hong
- Ching‑Hsein Chen
View Affiliations

Affiliations: Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, Chiayi, Taiwan, R.O.C., Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan, R.O.C. , Department of Physical Therapy, Hung Kuang University, Taichung, Taiwan, R.O.C., Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences, National Chiayi University, Chiayi, Taiwan, R.O.C.
Published online on: January 16, 2013 https://doi.org/10.3892/etm.2013.896
Pages: 964-968

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Propyl gallate (PG) is an antioxidant that has been used as an additive in several foods to protect against oxidation. The present study examined the anti‑inflammatory effect of PG on 12‑O‑tetradecanoylphorbol‑13‑acetate (TPA)‑induced inflammation in human THP‑1 monocytes. Pretreatment with PG markedly inhibited the TPA‑induced expression levels of cyclooxygenase‑2 and prostaglandin E2. The application of PG significantly inhibited the nuclear translocation of p65, a subunit of nuclear factor‑κB (NF‑κB) and phosphorylation of p65 (Ser536) in TPA‑treated THP‑1 cells. PG also inhibited the phosphorylation of IκB and IκB kinase. These results indicate that PG inhibits the inflammatory response by blocking the NF‑κB signaling pathway in TPA‑induced THP‑1 monocytes. Therefore, PG may be useful as a therapeutic agent in inflammatory diseases.

View Figures

View References

1.	Mutoh M, Takahashi M and Wakabayashi K: Roles of prostanoids in colon carcinogenesis and their potential targeting for cancer chemoprevention. Curr Pharm Des. 12:2375–2382. 2006. View Article : Google Scholar : PubMed/NCBI
2.	Piston D, Wang S, Feng Y, et al: The role of cyclooxygenase-2/prostanoid pathway in visceral pain induced liver stress response in rats. Chin Med J (Engl). 120:1813–1819. 2007.PubMed/NCBI
3.	Weerasinghe GR, Coon SL, Bhattacharjee AK, et al: Regional protein levels of cytosolic phospholipase A2 and cyclooxygenase-2 in Rhesus monkey brain as a function of age. Brain Res Bull. 69:614–621. 2006. View Article : Google Scholar : PubMed/NCBI
4.	Nagasaki S, Suzuki T, Miki Y, et al: 17Beta-hydroxysteroid dehydrogenase type 12 in human breast carcinoma: a prognostic factor via potential regulation of fatty acid synthesis. Cancer Res. 69:1392–1399. 2009. View Article : Google Scholar
5.	Branski RC, Zhou H, Sandulache VC, et al: Cyclooxygenase-2 signaling in vocal fold fibroblasts. Laryngoscope. 120:1826–1831. 2010. View Article : Google Scholar : PubMed/NCBI
6.	Oláh O, Németh I, Tóth-Szüki V, et al: Regional differences in the neuronal expression of cyclooxygenase-2 (COX-2) in the newborn pig brain. Acta Histochem Cytochem. 45:187–192. 2012.PubMed/NCBI
7.	Slattery ML, Lundgreen A, Bondurant KL and Wolff RK: Interferon-signaling pathway: associations with colon and rectal cancer risk and subsequent survival. Carcinogenesis. 32:1660–1667. 2011. View Article : Google Scholar : PubMed/NCBI
8.	Setia S and Sanyal SN: Nuclear factor kappa B: a pro-inflammatory, transcription factor-mediated signalling pathway in lung carcinogenesis and its inhibition by nonsteroidal anti-inflammatory drugs. J Environ Pathol Toxicol Oncol. 31:27–37. 2012. View Article : Google Scholar
9.	Liu D, Kim DH, Park JM, et al: Piceatannol inhibits phorbol ester-induced NF-kappa B activation and COX-2 expression in cultured human mammary epithelial cells. Nutr Cancer. 61:855–863. 2009. View Article : Google Scholar : PubMed/NCBI
10.	Fernández-Martínez AB, Carmena MJ, Arenas MI, et al: Overexpression of vasoactive intestinal peptide receptors and cyclooxygenase-2 in human prostate cancer. Analysis of potential prognostic relevance. Histol Histopathol. 27:1093–1101. 2012.
11.	Muller-Decker K, Neufang G, Berger I, et al: Transgenic cyclooxygenase-2 overexpression sensitizes mouse skin for carcinogenesis. Proc Natl Acad Sci USA. 99:12483–12488. 2002. View Article : Google Scholar : PubMed/NCBI
12.	Tiano HF, Loftin CD, Akunda J, et al: Deficiency of either cyclooxygenase (COX)-1 or COX-2 alters epidermal differentiation and reduces mouse skin tumorigenesis. Cancer Res. 62:3395–3401. 2002.PubMed/NCBI
13.	Cerella C, Sobolewski C, Chateauvieux S, et al: COX-2 inhibitors block chemotherapeutic agent-induced apoptosis prior to commitment in hematopoietic cancer cells. Biochem Pharmacol. 82:1277–1290. 2011. View Article : Google Scholar : PubMed/NCBI
14.	Gutierrez H, O’Keeffe GW, Gavaldà N, et al: Nuclear factor kappa B signaling either stimulates or inhibits neurite growth depending on the phosphorylation status of p65/RelA. J Neurosci. 28:8246–8256. 2008. View Article : Google Scholar : PubMed/NCBI
15.	Li X, Wu G, Wu M, et al: In vitro study of inhibitory millimeter wave treatment effects on the TNF-α-induced NF-κB signal transduction pathway. Int J Mol Med. 27:71–78. 2011.PubMed/NCBI
16.	Kole L, Giri B, Manna SK, et al: Biochanin-A, an isoflavon, showed anti-proliferative and anti-inflammatory activities through the inhibition of iNOS expression, p38-MAPK and ATF-2 phosphorylation and blocking NFκB nuclear translocation. Eur J Pharmacol. 653:8–15. 2011.PubMed/NCBI
17.	Kim SO, Kundu JK, Shin YK, et al: [6]-Gingerol inhibits COX-2 expression by blocking the activation of p38 MAP kinase and NF-kappaB in phorbol ester-stimulated mouse skin. Oncogene. 24:2558–2567. 2005.
18.	Kim HG, Han EH, Jang WS, et al: Piperine inhibits PMA-induced cyclooxygenase-2 expression through downregulating NF-κB, C/EBP and AP-1 signaling pathways in murine macrophages. Food Chem Toxicol. 50:2342–3428. 2012.PubMed/NCBI
19.	Granado-Serrano AB, Martín MÁ, Bravo L, et al: Quercetin attenuates TNF-induced inflammation in hepatic cells by inhibiting the NF-κB pathway. Nutr Cancer. 64:588–598. 2012.PubMed/NCBI
20.	Shen YC, Chen SL, Zhuang SR and Wang CK: Contribution of tomato phenolics to suppression of COX-2 expression in KB cells. J Food Sci. 73:C1–C10. 2008. View Article : Google Scholar : PubMed/NCBI
21.	Kim SH, Jun CD, Suk K, et al: Gallic acid inhibits histamine release and pro-inflammatory cytokine production in mast cells. Toxicol Sci. 91:123–131. 2006. View Article : Google Scholar : PubMed/NCBI
22.	Chen CH, Liu TZ, Chen CH, et al: The efficacy of protective effects of tannic acid, gallic acid, ellagic acid, and propyl gallate against hydrogen peroxide-induced oxidative stress and DNA damage in IMR-90 cells. Mol Nutr Food Res. 51:962–968. 2007. View Article : Google Scholar : PubMed/NCBI
23.	Arunkumar S, Ilango K, Manikandan RS and Ramalakshmi N: Synthesis and anti-inflammatory activity of some novel pyrazole derivatives of gallic acid. J Chem. 6(Suppl 1): S123–S128. 2009.