Establishment of canine models of lunatomalacia through liquid nitrogen freezing

Huang,Qishun; Fu,Qiang; Zheng,Huaiyuan; Gan,Meng; Wong,Yuxiong; Chen,Zhenbing

doi:10.3892/etm.2013.904

March 2013 Volume 5 Issue 3

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March 2013 Volume 5 Issue 3

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Article

Establishment of canine models of lunatomalacia through liquid nitrogen freezing

Authors:
- Qishun Huang
- Qiang Fu
- Huaiyuan Zheng
- Meng Gan
- Yuxiong Wong
- Zhenbing Chen
View Affiliations / Copyright

Affiliations: Department of Hand Surgery, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
Pages: 880-884
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Published online on: January 17, 2013

https://doi.org/10.3892/etm.2013.904
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Abstract

The aim of this study was to investigate the feasibility of establishing dog models of lunatomalacia through liquid nitrogen freezing. Twelve adult crossbred dogs were divided into three groups. Unilateral lunates were peeled off the parenchyma and frozen to result in avascular necrosis. They were observed dynamically through X-ray, computed tomography (CT) and magnetic resonance imaging (MRI). Furthermore, gross and histomorphological observations of samples were performed. Disseminated punctate hyperintense images and abnormal manifestations were detected, respectively. At 12 weeks after surgery, uneven bone density of the lunate and a flattened lunate of irregular shape were detected. A large area of irregular hypointense foci and hyperintensity was observed. Gross sample observation revealed a large area of dead bone. A decrease in the density of the trabecular bones and several vacant bone lacunas were visible. Liquid nitrogen freezing is a successful and reliable method for preparing animal models of lunatomalacia.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Establishment of canine models of lunatomalacia through liquid nitrogen freezing

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