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Article

Opposite effect of angiotensin receptor blockade on CXCL8 production and CXCR1/2 expression of angiotensin II‑treated THP‑1 monocytes

  • Authors:
    • Konstantina Vogiatzi
    • Stavros Apostolakis
    • Zaharenia Vlata
    • Elias Krabovitis
    • Demetrios A. Spandidos
  • View Affiliations / Copyright

    Affiliations: Laboratory of Clinical Virology, Faculty of Medicine University of Crete, Heraklion 71003, Crete, Greece, Institute of Molecular Biology and Biotechnology, Department of Applied Bioschemistry and Immunology, Heraklion 71003, Crete, Greece
  • Pages: 987-991
    |
    Published online on: January 21, 2013
       https://doi.org/10.3892/etm.2013.909
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Abstract

Interleukin‑8 (IL‑8) or CXCL8 is a potent chemotactic factor that is involved in atherogenesis. IL‑8 mediates its pre‑inflammatory effects through interaction with CXCR1 and CXCR2. In the present study, we investigated the effects of angiotensin II (Ang II) on IL‑8 synthesis and CXCR1/CXCR2 expression of THP‑1 monocytes. IL‑8 was measured in the culture medium using ELISA. Expression of chemokine receptors CXCR1 and CXCR2 was evaluated by flow cytometry. Results demonstrated that the addition of Ang II increased IL‑8 production in the THP‑1 monocytes. The Ang II type 1 receptor blocker (ARB) losartan significantly blocked Ang II‑induced IL‑8 production. Notably, losartan blocked LPS‑induced IL‑8 production by THP‑1 monocytes and produced a small but statistically significant reduction of baseline IL‑8 production of naïve THP‑1 cells. Losartan also produced a statistically significant increase of fluorescence intensity of naïve CXCR1‑ and CXCR2‑positive THP‑1 monocytes, probably as a negative feedback effect secondary to IL‑8 downregulation. In conclusion, we demonstrated that Ang II increased IL‑8 production by THP‑1 monocytes. Losartan significantly suppressed the latter effect, suggesting an AT‑1 mediated pathway. Moreover, losartan suppressed the IL‑8 production of naïve THP‑1 monocytes and LPS‑treated THP‑1 monocytes, suggesting a broader spectrum of pleiotropic effects. Extrapolating this in vitro observation to in vivo pathways, we propose Ang II‑induced IL‑8 production by monocytes as another pre‑atherogenic potential of Ang II that can be effectively blocked by the AT1 receptor blockade.
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Copy and paste a formatted citation
Spandidos Publications style
Vogiatzi K, Apostolakis S, Vlata Z, Krabovitis E and Spandidos DA: Opposite effect of angiotensin receptor blockade on CXCL8 production and CXCR1/2 expression of angiotensin II‑treated THP‑1 monocytes. Exp Ther Med 5: 987-991, 2013.
APA
Vogiatzi, K., Apostolakis, S., Vlata, Z., Krabovitis, E., & Spandidos, D.A. (2013). Opposite effect of angiotensin receptor blockade on CXCL8 production and CXCR1/2 expression of angiotensin II‑treated THP‑1 monocytes. Experimental and Therapeutic Medicine, 5, 987-991. https://doi.org/10.3892/etm.2013.909
MLA
Vogiatzi, K., Apostolakis, S., Vlata, Z., Krabovitis, E., Spandidos, D. A."Opposite effect of angiotensin receptor blockade on CXCL8 production and CXCR1/2 expression of angiotensin II‑treated THP‑1 monocytes". Experimental and Therapeutic Medicine 5.3 (2013): 987-991.
Chicago
Vogiatzi, K., Apostolakis, S., Vlata, Z., Krabovitis, E., Spandidos, D. A."Opposite effect of angiotensin receptor blockade on CXCL8 production and CXCR1/2 expression of angiotensin II‑treated THP‑1 monocytes". Experimental and Therapeutic Medicine 5, no. 3 (2013): 987-991. https://doi.org/10.3892/etm.2013.909
Copy and paste a formatted citation
x
Spandidos Publications style
Vogiatzi K, Apostolakis S, Vlata Z, Krabovitis E and Spandidos DA: Opposite effect of angiotensin receptor blockade on CXCL8 production and CXCR1/2 expression of angiotensin II‑treated THP‑1 monocytes. Exp Ther Med 5: 987-991, 2013.
APA
Vogiatzi, K., Apostolakis, S., Vlata, Z., Krabovitis, E., & Spandidos, D.A. (2013). Opposite effect of angiotensin receptor blockade on CXCL8 production and CXCR1/2 expression of angiotensin II‑treated THP‑1 monocytes. Experimental and Therapeutic Medicine, 5, 987-991. https://doi.org/10.3892/etm.2013.909
MLA
Vogiatzi, K., Apostolakis, S., Vlata, Z., Krabovitis, E., Spandidos, D. A."Opposite effect of angiotensin receptor blockade on CXCL8 production and CXCR1/2 expression of angiotensin II‑treated THP‑1 monocytes". Experimental and Therapeutic Medicine 5.3 (2013): 987-991.
Chicago
Vogiatzi, K., Apostolakis, S., Vlata, Z., Krabovitis, E., Spandidos, D. A."Opposite effect of angiotensin receptor blockade on CXCL8 production and CXCR1/2 expression of angiotensin II‑treated THP‑1 monocytes". Experimental and Therapeutic Medicine 5, no. 3 (2013): 987-991. https://doi.org/10.3892/etm.2013.909
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