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Article

Reduced expression levels of the death‑associated protein kinase and E-cadherin are correlated with the development of esophageal squamous cell carcinoma

  • Authors:
    • Jianwen Zhai
    • Xiaogang Yang
    • Yanli Zhang
    • Qingbin Qi
    • Jigang Hu
    • Qiaomei Wang
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Affiliated Hospital of Hebei Engineering University, Handan, Hebei 056002, P.R. China
  • Pages: 972-976
    |
    Published online on: January 22, 2013
       https://doi.org/10.3892/etm.2013.916
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Abstract

The aim of this study was to investigate the expression levels of the death‑associated protein kinase (DAPK) and E-cadherin in esophageal squamous cell carcinoma (ESCC) and their correlation with clinical and pathological factors. Immunohistochemistry [streptavidin-peroxidase (SP) method], in situ hybridization, immunoblot assays and reverse transcription-PCR (RT-PCR) were used to detect the expression of DAPK and E-cadherin in the carcinomas and the adjacent normal tissues of 76 cases of esophageal squamous carcinomas. The immunoblot assay indicated that the expression levels of DAPK and E-cadherin were decreased significantly in the ESCC tissue (P<0.05) when compared with the adjacent normal tissues. The RT-PCR results showed that the mRNA levels of DAPK and E-cadherin were reduced. The abnormal expression of DAPK was highly correlated with the invasiveness and lymphatic metastasis of the cancer. The abnormal expression of E-cadherin was highly correlated with the differentiation and lymphatic metastasis of the cancer. The decreased expression levels of DAPK and E-cadherin correlated with the development of ESCC. The combined detection of DAPK and E-cadherin proteins may be correlated with the degree of malignancy and metastatic potency of ESCC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhai J, Yang X, Zhang Y, Qi Q, Hu J and Wang Q: Reduced expression levels of the death‑associated protein kinase and E-cadherin are correlated with the development of esophageal squamous cell carcinoma. Exp Ther Med 5: 972-976, 2013.
APA
Zhai, J., Yang, X., Zhang, Y., Qi, Q., Hu, J., & Wang, Q. (2013). Reduced expression levels of the death‑associated protein kinase and E-cadherin are correlated with the development of esophageal squamous cell carcinoma. Experimental and Therapeutic Medicine, 5, 972-976. https://doi.org/10.3892/etm.2013.916
MLA
Zhai, J., Yang, X., Zhang, Y., Qi, Q., Hu, J., Wang, Q."Reduced expression levels of the death‑associated protein kinase and E-cadherin are correlated with the development of esophageal squamous cell carcinoma". Experimental and Therapeutic Medicine 5.3 (2013): 972-976.
Chicago
Zhai, J., Yang, X., Zhang, Y., Qi, Q., Hu, J., Wang, Q."Reduced expression levels of the death‑associated protein kinase and E-cadherin are correlated with the development of esophageal squamous cell carcinoma". Experimental and Therapeutic Medicine 5, no. 3 (2013): 972-976. https://doi.org/10.3892/etm.2013.916
Copy and paste a formatted citation
x
Spandidos Publications style
Zhai J, Yang X, Zhang Y, Qi Q, Hu J and Wang Q: Reduced expression levels of the death‑associated protein kinase and E-cadherin are correlated with the development of esophageal squamous cell carcinoma. Exp Ther Med 5: 972-976, 2013.
APA
Zhai, J., Yang, X., Zhang, Y., Qi, Q., Hu, J., & Wang, Q. (2013). Reduced expression levels of the death‑associated protein kinase and E-cadherin are correlated with the development of esophageal squamous cell carcinoma. Experimental and Therapeutic Medicine, 5, 972-976. https://doi.org/10.3892/etm.2013.916
MLA
Zhai, J., Yang, X., Zhang, Y., Qi, Q., Hu, J., Wang, Q."Reduced expression levels of the death‑associated protein kinase and E-cadherin are correlated with the development of esophageal squamous cell carcinoma". Experimental and Therapeutic Medicine 5.3 (2013): 972-976.
Chicago
Zhai, J., Yang, X., Zhang, Y., Qi, Q., Hu, J., Wang, Q."Reduced expression levels of the death‑associated protein kinase and E-cadherin are correlated with the development of esophageal squamous cell carcinoma". Experimental and Therapeutic Medicine 5, no. 3 (2013): 972-976. https://doi.org/10.3892/etm.2013.916
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