The role of the Rho/Rock signaling pathway in the pathogenesis of acute ischemic myocardial fibrosis in rat models

Gao,Hai-Cheng; Zhao,Hang; Zhang,Wen-Qi; Li,Yun-Qian; Ren,Li-Qun

doi:10.3892/etm.2013.935

April 2013 Volume 5 Issue 4

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April 2013 Volume 5 Issue 4

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Article

The role of the Rho/Rock signaling pathway in the pathogenesis of acute ischemic myocardial fibrosis in rat models

Authors:
- Hai-Cheng Gao
- Hang Zhao
- Wen-Qi Zhang
- Yun-Qian Li
- Li-Qun Ren
View Affiliations / Copyright

Affiliations: Department of Clinical Pharmacy and Pharmaceutical Management, Jilin University, Changchun 130021, P.R. China, Department of Neurosurgery, The First Hospital of Jilin University, Changchun 130021, P.R. China, Department of Cardiology, China-Japan Union Hospital of Jilin University, Changchun 130033, P.R. China
Pages: 1123-1128
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Published online on: January 30, 2013

https://doi.org/10.3892/etm.2013.935
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Abstract

The aim of this study was to investigate the role of the Rho/Rho associated coiled coil-forming protein kinase (Rock) signaling pathway in the pathogenesis of ischemic myocardial fibrosis (MF) in rats. The MF rat model was established using isoprenaline hydrochloride (ISO, 15 mg/kg). Rats were randomly divided into ten groups: a control group and ISO-treated groups at 2 h, 4 h, 6 h, 12 h, 24 h, 48 h, 72 h, 7 days and 21 days . The MF model was evaluated by serum enzyme levels, hematoxylin and eosin (H&E) staining and Masson's staining, ex vivo. The mRNA expression of RhoA and Rock I was assessed with reverse transcription-polymerase chain reaction (RT-PCR). The cell type was evaluated by immunofluorescent and immunohistochemical staining. The protein expression of Rock I was evaluated using western blotting and immunohistochemistry. MF was found to be more developed in the ISO-treated group compared with the control group. CD31 and vimentin expression in fibroblasts and endothelial cells were significantly increased. In addition, the mRNA and protein levels of RhoA and Rock I were significantly increased. In conclusion, activation of Rho/Rock accelerates the degree of ischemic MF. Inhibition of Rho/Rock may be a novel therapeutic strategy for the prevention of ischemic MF.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

The role of the Rho/Rock signaling pathway in the pathogenesis of acute ischemic myocardial fibrosis in rat models

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