Tumor suppressive microRNA‑424 inhibits osteosarcoma cell migration and invasion via targeting fatty acid synthase

  • Authors:
    • Xing Hua Long
    • Jian Hua Mao
    • Ai Fen Peng
    • Yang Zhou
    • Shan Hu Huang
    • Zhi Li Liu
  • View Affiliations

  • Published online on: February 18, 2013     https://doi.org/10.3892/etm.2013.959
  • Pages: 1048-1052
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Numerous studies have recently suggested that miRNAs contribute to the development of various types of human cancer as well as to their invasive and metastatic capacities. The aim of this study was to investigate the functional significance of miR‑424 and to identify its possible target genes in osteosarcoma (OS) cells. Previously, inhibition of fatty acid synthase (FASN) has been shown to suppress OS cell proliferation, invasion and migration. The prediction was made using the microRNA.org and TargetScan.human6.0.database. The results showed that FASN is a promising target gene of miR‑424. FASN may be a direct target of miR-424 as shown by the luciferase reporter assays. Furthermore, miR-424 expression was increased in osteosarcoma cells by transfection with has-miR-424. FASN mRNA and protein expression levels were measured by RT-PCR and western blot analysis. Cell migration and invasion was measured using Transwell migration and Transwell invasion assays. Expression levels of FASN mRNA and protein were greatly decreased in U2OS cells transfected with has-miR-424. The migration and invasion of cells was significantly decreased by the upregulation of miR-424. These findings suggested that miR-424 plays a key role in inhibiting OS cell migration and invasion through targeting FASN.
View Figures
View References

Related Articles

Journal Cover

April 2013
Volume 5 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Long XH, Mao JH, Peng AF, Zhou Y, Huang SH and Liu ZL: Tumor suppressive microRNA‑424 inhibits osteosarcoma cell migration and invasion via targeting fatty acid synthase. Exp Ther Med 5: 1048-1052, 2013
APA
Long, X.H., Mao, J.H., Peng, A.F., Zhou, Y., Huang, S.H., & Liu, Z.L. (2013). Tumor suppressive microRNA‑424 inhibits osteosarcoma cell migration and invasion via targeting fatty acid synthase. Experimental and Therapeutic Medicine, 5, 1048-1052. https://doi.org/10.3892/etm.2013.959
MLA
Long, X. H., Mao, J. H., Peng, A. F., Zhou, Y., Huang, S. H., Liu, Z. L."Tumor suppressive microRNA‑424 inhibits osteosarcoma cell migration and invasion via targeting fatty acid synthase". Experimental and Therapeutic Medicine 5.4 (2013): 1048-1052.
Chicago
Long, X. H., Mao, J. H., Peng, A. F., Zhou, Y., Huang, S. H., Liu, Z. L."Tumor suppressive microRNA‑424 inhibits osteosarcoma cell migration and invasion via targeting fatty acid synthase". Experimental and Therapeutic Medicine 5, no. 4 (2013): 1048-1052. https://doi.org/10.3892/etm.2013.959