Studies on the effect of adenosine on calcium oscillation in hippocampal neurons

Chen,Jinbo; Wang,Yuan; Wang,Yuliang; Yi,Xiangming; Ge,Ruli

doi:10.3892/etm.2013.973

April 2013 Volume 5 Issue 4

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April 2013 Volume 5 Issue 4

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Article

Studies on the effect of adenosine on calcium oscillation in hippocampal neurons

Authors:
- Jinbo Chen
- Yuan Wang
- Yuliang Wang
- Xiangming Yi
- Ruli Ge
View Affiliations / Copyright

Affiliations: Department of Neurology, The Affiliated Hospital of Binzhou Medical University, Binzhou 256603, P.R. China
Pages: 1165-1168
|
Published online on: February 25, 2013

https://doi.org/10.3892/etm.2013.973
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Abstract

Adenosine (Ade) is an antiepileptic agent. In order to investigate the possible mechanism of action of Ade, its effect on calcium (Ca2+) oscillations in hippocampal neurons of Sprague Dawley (SD) rats was explored. Primary hippocampal neurons were cultured from suckling neonatal SD rats. Cells were cultured for 7-9 days and the Ca2+ oscillations in response to perfusion with Ade were detected using confocal laser scanning microscopy in combination with Fluo-3/AM labeling. This study found that Ade inhibits the spontaneous synchronized Ca2+ oscillation frequency and amplitude in mature hippocampal neurons and such inhibition depends on the Ade dosage level to a certain extent. Ade also had a significant inhibitory effect on high potassium-induced Ca2+ oscillation frequency and amplitude. Ade had a significant inhibitory effect on high-voltage-activated Ca2+ channel-mediated Ca2+ influx and Ca2+ oscillations in neurons. This may be one of the mechanisms for Ade to exert antiepileptic effects as an endogenous substance.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Studies on the effect of adenosine on calcium oscillation in hippocampal neurons

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