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Print ISSN: 1792-0981 Online ISSN: 1792-1015
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2014-March Volume 7 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

Differentiation of human embryonic germ cells and transplantation in rats with acute myocardial infarction

  • Authors:
    • Shuichang Yu
    • Yanbo Zhu
    • Fang Li
    • Yujuan Zhang
    • Chunlin Xia
  • View Affiliations / Copyright

    Affiliations: Department of Histology and Embryology, Medical College of Soochow University, Suzhou 215123, P.R. China, Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou 215006, P.R. China, Department of Histology and Embryology, Medical College of Soochow University, Suzhou 215123, P.R. China, Boxi Institute of Clinical Anatomy and Cytoneurobiology Laboratory, Medical College of Soochow University, Suzhou 215123, P.R. China
  • Pages: 615-620
    |
    Published online on: January 3, 2014
       https://doi.org/10.3892/etm.2014.1474
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Abstract

Human embryonic germ cells (hEGCs) are stem cells cultured from primordial germ cells, which reside in human embryonic genital ridges in vivo. In this study, hEGCs were induced to differentiate into cardiomyocytes by treatment with ascorbic acid in vitro and the effects of hEGC transplantation on rat models of acute myocardial infarction (AMI) were investigated. hEGCs were incubated with differentiation medium containing ascorbic acid at various concentrations. Levels of GATA-4 expression were measured to identify the optimal concentration of the inductor. Immunofluorescence microscopy was used to detect the expression of Cx43 on the induced cells. The hEGCs were injected into the myocardium of rats with AMI. The expression levels of MAB1281 and GATA-4 were used to indicate the survival, migration, distribution and differentiation of transplanted cells. The results revealed the positive expression of GATA-4, Cx43 and cardiac troponin T (cTnT) in differentiated cells, and immunocytochemistry showed that transplanted cells highly expressed GATA-4 and MAB1281. hEGCs were successfully induced to differentiate into cardiomyocytes by ascorbic acid in optimal concentrations in vitro and the transplanted hEGCs survived and differentiated into cardiomyocytes.
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Copy and paste a formatted citation
Spandidos Publications style
Yu S, Zhu Y, Li F, Zhang Y and Xia C: Differentiation of human embryonic germ cells and transplantation in rats with acute myocardial infarction. Exp Ther Med 7: 615-620, 2014.
APA
Yu, S., Zhu, Y., Li, F., Zhang, Y., & Xia, C. (2014). Differentiation of human embryonic germ cells and transplantation in rats with acute myocardial infarction. Experimental and Therapeutic Medicine, 7, 615-620. https://doi.org/10.3892/etm.2014.1474
MLA
Yu, S., Zhu, Y., Li, F., Zhang, Y., Xia, C."Differentiation of human embryonic germ cells and transplantation in rats with acute myocardial infarction". Experimental and Therapeutic Medicine 7.3 (2014): 615-620.
Chicago
Yu, S., Zhu, Y., Li, F., Zhang, Y., Xia, C."Differentiation of human embryonic germ cells and transplantation in rats with acute myocardial infarction". Experimental and Therapeutic Medicine 7, no. 3 (2014): 615-620. https://doi.org/10.3892/etm.2014.1474
Copy and paste a formatted citation
x
Spandidos Publications style
Yu S, Zhu Y, Li F, Zhang Y and Xia C: Differentiation of human embryonic germ cells and transplantation in rats with acute myocardial infarction. Exp Ther Med 7: 615-620, 2014.
APA
Yu, S., Zhu, Y., Li, F., Zhang, Y., & Xia, C. (2014). Differentiation of human embryonic germ cells and transplantation in rats with acute myocardial infarction. Experimental and Therapeutic Medicine, 7, 615-620. https://doi.org/10.3892/etm.2014.1474
MLA
Yu, S., Zhu, Y., Li, F., Zhang, Y., Xia, C."Differentiation of human embryonic germ cells and transplantation in rats with acute myocardial infarction". Experimental and Therapeutic Medicine 7.3 (2014): 615-620.
Chicago
Yu, S., Zhu, Y., Li, F., Zhang, Y., Xia, C."Differentiation of human embryonic germ cells and transplantation in rats with acute myocardial infarction". Experimental and Therapeutic Medicine 7, no. 3 (2014): 615-620. https://doi.org/10.3892/etm.2014.1474
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