Effect of cilostazol pretreatment on the PARP/AIF‑mediated apoptotic pathway in rat cerebral ischemia‑reperfusion models

Ba,Xiao‑Hong; Cai,Li‑Ping; Han,Wei

doi:10.3892/etm.2014.1551

May-2014 Volume 7 Issue 5

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May-2014 Volume 7 Issue 5

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Article

Effect of cilostazol pretreatment on the PARP/AIF‑mediated apoptotic pathway in rat cerebral ischemia‑reperfusion models

Authors:
- Xiao‑Hong Ba
- Li‑Ping Cai
- Wei Han
View Affiliations / Copyright

Affiliations: Department of Neurology, The First Affiliated Hospital, Liaoning Medical University, Jinzhou, Liaoning 121001, P.R. China, Department of Neurology, Liaoning Provincial Corps Hospital, Chinese People's Armed Police Force, Shenyang, Liaoning 110034, P.R. China
Pages: 1209-1214
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Published online on: February 17, 2014

https://doi.org/10.3892/etm.2014.1551
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Abstract

The aim of this study was to observe the expression of poly ADP‑ribose polymerase (PARP) and apoptosis‑inducing factor (AIF) in the CA1 region of the hippocampus and to explore whether cilostazol pretreatment exerts a protective effect on the brain through the PARP/AIF‑mediated pathway in a rat model of cerebral ischemia‑reperfusion. Rats were randomly divided into three groups: Sham‑surgery, ischemia‑reperfusion and cilostazol (n=45 rats/group). Rat models of middle cerebral artery occlusion were prepared using a thread occlusion method. Rats in the cilostazol group were administered 30 mg/kg intragastric cilostazol 6 and 2 h before brain ischemia, respectively. Following reperfusion, samples were collected at different time‑points (6, 24 and 72 h) and each group was further subdivided into three subgroups (n=15 rats/subgroup). Apoptosis was measured using the terminal deoxynucleotidyl‑transferase‑mediated dUTP nick end labeling method. The protein expression levels of AIF and PARP were detected using western blot analysis and the expression levels of AIF mRNA were determined using the reverse transcription‑polymerase chain reaction. AIF nuclear translocation occurred following local cerebral ischemia‑reperfusion injury. Apoptosis, levels of AIF and PARP protein expression and levels of AIF mRNA expression were significantly increased in the ischemia‑reperfusion group compared with the sham‑surgery group (P<0.05). However, apoptosis and the expression levels of AIF protein, PARP protein and AIF mRNA at different time‑points were significantly decreased in the cilostazol group compared with the ischemia‑reperfusion group (P<0.05). In conclusion, cilostazol has a protective effect on rat cerebral ischemia‑reperfusion injury, and acts by inhibiting nerve cell apoptosis by preventing the excessive activation of PARP and AIF nuclear translocation.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effect of cilostazol pretreatment on the PARP/AIF‑mediated apoptotic pathway in rat cerebral ischemia‑reperfusion models

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