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Article

Total flavonoids from Plumula Nelumbinis suppress angiotensin II‑induced fractalkine production by inhibiting the ROS/NF‑κB pathway in human umbilical vein endothelial cells

  • Authors:
    • Yongwen Cao
    • Lulu Zheng
    • Shao Liu
    • Zhenyu Peng
    • Saidan Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China, Department of Pharmacology, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China, Department of Emergency, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China
  • Pages: 1187-1192
    |
    Published online on: February 17, 2014
       https://doi.org/10.3892/etm.2014.1554
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Abstract

Angiotensin II (Ang II) is a neuroendocrine factor that promotes hypertension and has been implicated in vascular inflammation through the induction of reactive oxygen species (ROS) and proinflammatory genes in endothelial cells. However, relatively little attention has been paid to the effect of Ang II on fractalkine (FKN), an important chemokine involved in endothelial dysfunction. In the study, we aimed to investigate the protective role of total flavonoids from Plumula Nelumbinis (TFPN), the main component extracted from Semen Nelumbinis, in Ang II-induced oxidative stress injury in human umbilical vein endothelial cells (HUVECs). Furthermore, we studied whether TFPN could attenuate the Ang II-induced generation of ROS and the activation of nuclear factor-κB (NF-κB); whether these Ang II-induced effects were inhibited by apocynin (a nicotinamide adenine dinucleotide phosphate oxidase inhibitor) and pyrrolidine dithiocarbamate (an NF-κB inhibitor). In the present study, it was observed that total flavonoids from Plumula Nelumbinis (TFPN), the main component extracted from Semen Nelumbinis, concentration‑dependently inhibited the FKN production induced by Ang II in human umbilical vein endothelial cells (HUVECs). Furthermore, TFPN attenuated the Ang II‑induced generation of ROS and the activation of nuclear factor‑κB (NF‑κB); these Ang II‑induced effects were also inhibited by apocynin (a nicotinamide adenine dinucleotide phosphate oxidase inhibitor) and pyrrolidine dithiocarbamate (an NF‑κB inhibitor). In conclusion, the findings of the present study indicate that TFPN attenuate Ang II‑induced upregulation of FKN by inhibiting the ROS/NF‑κB pathway in HUVECs and thus have a suppressive effect on vascular inflammation.
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1 

Rajagopalan S, Kurz S, Münzel T, et al: Angiotensin II-mediated hypertension in the rat increases vascular superoxide production via membrane NADH/NADPH oxidase activation. Contribution to alterations of vasomotor tone. J Clin Invest. 97:1916–1923. 1996. View Article : Google Scholar

2 

Touyz RM and Briones AM: Reactive oxygen species and vascular biology: implications in human hypertension. Hypertens Res. 34:5–14. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Wang J, Hu X, Yin W and Cai H: Alkaloids of plumula Nelumbinis. Zhongguo Zhong Yao Za Zhi. 16:673–675. 7031991.(In Chinese).

4 

Lebeau J, Furman C, Bernier JL, Duriez P, Teissier E and Cotelle N: Antioxidant properties of di-tert-butylhydroxylated flavonoids. Free Radic Biol Med. 29:900–912. 2000. View Article : Google Scholar : PubMed/NCBI

5 

Owen RW, Haubner R, Mier W, et al: Isolation, structure elucidation and antioxidant potential of the major phenolic and flavonoid compounds in brined olive drupes. Food Chem Toxicol. 41:703–717. 2003. View Article : Google Scholar

6 

Bergman M, Perelman A, Dubinsky Z and Grossman S: Scavenging of reactive oxygen species by a novel glucurinated flavonoid antioxidant isolated and purified from spinach. Phytochemistry. 62:753–762. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Dugas AJ Jr, Castañeda-Acosta J, Bonin GC, Price KL, Fischer NH and Winston GW: Evaluation of the total peroxyl radical-scavenging capacity of flavonoids: structure-activity relationships. J Nat Prod. 63:327–331. 2000. View Article : Google Scholar : PubMed/NCBI

8 

Mishra B, Priyadarsini KI, Kumar MS, Unnikrishnan MK and Mohan H: Effect of O-glycosylation on the antioxidant activity and free radical reactions of a plant flavonoid, chrysoeriol. Bioorg Med Chem. 11:2677–2685. 2003. View Article : Google Scholar : PubMed/NCBI

9 

Jia SJ, Jiang DJ, Hu CP, et al: Lysophosphatidylcholine-induced elevation of asymmetric dimethylarginine level by the NADPH oxidase pathway in endothelial cells. Vascul Pharmacol. 44:143–148. 2006. View Article : Google Scholar

10 

Park JB, Charbonneau F and Schiffrin EL: Correlation of endothelial function in large and small arteries in human essential hypertension. J Hypertens. 19:415–420. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Tomita N, Yamasaki K, Osako MK, Komai N, Shimosato T and Morishita R: Combination therapy based on the angiotensin receptor blocker olmesartan for vascular protection in spontaneously hypertensive rats. Mol Med Rep. 2:733–738. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Guzik TJ, West NE, Black E, et al: Vascular superoxide production by NAD(P)H oxidase: association with endothelial dysfunction and clinical risk factors. Circ Res. 86:E85–E90. 2000. View Article : Google Scholar

13 

Cai H and Harrison DG: Endothelial dysfunction in cardiovascular diseases: the role of oxidant stress. Circ Res. 87:840–844. 2000. View Article : Google Scholar : PubMed/NCBI

14 

Guzik TJ, Hoch NE, Brown KA, et al: Role of the T cell in the genesis of angiotensin II induced hypertension and vascular dysfunction. J Exp Med. 204:2449–2460. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Griendling KK, Minieri CA, Ollerenshaw JD and Alexander RW: Angiotensin II stimulates NADH and NADPH oxidase activity in cultured vascular smooth muscle cells. Circ Res. 74:1141–1148. 1994. View Article : Google Scholar : PubMed/NCBI

16 

Shi Y, Niculescu R, Wang D, Patel S, Davenpeck KL and Zalewski A: Increased NAD(P)H oxidase and reactive oxygen species in coronary arteries after balloon injury. Arterioscler Thromb Vasc Biol. 21:739–745. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Ohtsu H, Frank GD, Utsunomiya H and Eguchi S: Redox-dependent protein kinase regulation by angiotensin II: mechanistic insights and its pathophysiology. Antioxid Redox Signal. 7:1315–1326. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Lassègue B and Clempus RE: Vascular NAD(P)H oxidases: specific features, expression, and regulation. Am J Physiol Regul Integr Comp Physiol. 285:R277–R297. 2003.PubMed/NCBI

19 

Kalinowski L and Malinski T: Endothelial NADH/NADPH-dependent enzymatic sources of superoxide production: relationship to endothelial dysfunction. Acta Biochim Pol. 51:459–469. 2004.PubMed/NCBI

20 

Chandrasekar B, Mummidi S, Perla RP, et al: Fractalkine (CX3CL1) stimulated by nuclear factor kappaB (NF-kappaB)-dependent inflammatory signals induces aortic smooth muscle cell proliferation through an autocrine pathway. Biochem J. 373:547–558. 2003. View Article : Google Scholar

21 

Garcia GE, Xia Y, Chen S, et al: NF-kappaB-dependent fractalkine induction in rat aortic endothelial cells stimulated by IL-1beta, TNF-alpha, and LPS. J Leukoc Biol. 67:577–584. 2000.PubMed/NCBI

22 

Zernecke A, Weber KS and Weber C: Combined modulation of the mesangial machinery for monocyte recruitment by inhibition of NF-kappaB. Am J Physiol Cell Physiol. 281:C1881–C1888. 2001.

23 

Pomerantz JL and Baltimore D: Two pathways to NF-kappaB. Mol Cell. 10:693–695. 2002. View Article : Google Scholar : PubMed/NCBI

24 

Bonello S, Zähringer C, BelAiba RS, et al: Reactive oxygen species activate the HIF-1alpha promoter via a functional NFkappaB site. Arterioscler Thromb Vasc Biol. 27:755–761. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Yokoo T and Kitamura M: Antioxidant PDTC induces stromelysin expression in mesangial cells via a tyrosine kinase-AP-1 pathway. Am J Physiol. 270:F806–F811. 1996.PubMed/NCBI

26 

Chen J, Zhang M, Zheng T-S and Tao J-H: Study on antioxidant activities of ethanol extracts from lotus germ in vitro. Food Science. 29:48–51. 2008.(In Chinese).

27 

Hanna IR, Taniyama Y, Szöcs K, Rocic P and Griendling KK: NAD(P)H oxidase-derived reactive oxygen species as mediators of angiotensin II signaling. Antioxid Redox Signal. 4:899–914. 2002. View Article : Google Scholar : PubMed/NCBI

28 

Ruiz-Ortega M, Ruperez M, Esteban V and Egido J: Molecular mechanisms of angiotensin II-induced vascular injury. Curr Hypertens Rep. 5:73–79. 2003. View Article : Google Scholar : PubMed/NCBI

29 

Luo Z, Teerlink T, Griendling K, Aslam S, Welch WJ and Wilcox CS: Angiotensin II and NADPH oxidase increase ADMA in vascular smooth muscle cells. Hypertension. 56:498–504. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Wong BW, Wong D and McManus BM: Characterization of fractalkine (CX3CL1) and CX3CR1 in human coronary arteries with native atherosclerosis, diabetes mellitus, and transplant vascular disease. Cardiovasc Pathol. 11:332–338. 2002. View Article : Google Scholar : PubMed/NCBI

31 

Sullivan JC, Pardieck JL, Doran D, Zhang Y, She JX and Pollock JS: Greater fractalkine expression in mesenteric arteries of female spontaneously hypertensive rats compared with males. Am J Physiol Heart Circ Physiol. 296:H1080–H1088. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Schäfer A, Schulz C, Fraccarollo D, et al: The CX3C chemokine fractalkine induces vascular dysfunction by generation of superoxide anions. Arterioscler Thromb Vasc Biol. 27:55–62. 2007.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Cao Y, Zheng L, Liu S, Peng Z and Zhang S: Total flavonoids from Plumula Nelumbinis suppress angiotensin II‑induced fractalkine production by inhibiting the ROS/NF‑κB pathway in human umbilical vein endothelial cells. Exp Ther Med 7: 1187-1192, 2014.
APA
Cao, Y., Zheng, L., Liu, S., Peng, Z., & Zhang, S. (2014). Total flavonoids from Plumula Nelumbinis suppress angiotensin II‑induced fractalkine production by inhibiting the ROS/NF‑κB pathway in human umbilical vein endothelial cells. Experimental and Therapeutic Medicine, 7, 1187-1192. https://doi.org/10.3892/etm.2014.1554
MLA
Cao, Y., Zheng, L., Liu, S., Peng, Z., Zhang, S."Total flavonoids from Plumula Nelumbinis suppress angiotensin II‑induced fractalkine production by inhibiting the ROS/NF‑κB pathway in human umbilical vein endothelial cells". Experimental and Therapeutic Medicine 7.5 (2014): 1187-1192.
Chicago
Cao, Y., Zheng, L., Liu, S., Peng, Z., Zhang, S."Total flavonoids from Plumula Nelumbinis suppress angiotensin II‑induced fractalkine production by inhibiting the ROS/NF‑κB pathway in human umbilical vein endothelial cells". Experimental and Therapeutic Medicine 7, no. 5 (2014): 1187-1192. https://doi.org/10.3892/etm.2014.1554
Copy and paste a formatted citation
x
Spandidos Publications style
Cao Y, Zheng L, Liu S, Peng Z and Zhang S: Total flavonoids from Plumula Nelumbinis suppress angiotensin II‑induced fractalkine production by inhibiting the ROS/NF‑κB pathway in human umbilical vein endothelial cells. Exp Ther Med 7: 1187-1192, 2014.
APA
Cao, Y., Zheng, L., Liu, S., Peng, Z., & Zhang, S. (2014). Total flavonoids from Plumula Nelumbinis suppress angiotensin II‑induced fractalkine production by inhibiting the ROS/NF‑κB pathway in human umbilical vein endothelial cells. Experimental and Therapeutic Medicine, 7, 1187-1192. https://doi.org/10.3892/etm.2014.1554
MLA
Cao, Y., Zheng, L., Liu, S., Peng, Z., Zhang, S."Total flavonoids from Plumula Nelumbinis suppress angiotensin II‑induced fractalkine production by inhibiting the ROS/NF‑κB pathway in human umbilical vein endothelial cells". Experimental and Therapeutic Medicine 7.5 (2014): 1187-1192.
Chicago
Cao, Y., Zheng, L., Liu, S., Peng, Z., Zhang, S."Total flavonoids from Plumula Nelumbinis suppress angiotensin II‑induced fractalkine production by inhibiting the ROS/NF‑κB pathway in human umbilical vein endothelial cells". Experimental and Therapeutic Medicine 7, no. 5 (2014): 1187-1192. https://doi.org/10.3892/etm.2014.1554
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