Vacuum‑assisted closure increases ICAM‑1, MIF, VEGF and collagen I expression in wound therapy

Wang,Weiyang; Pan,Zhenyu; Hu,Xiang; Li,Zonghuan; Zhao,Yong; Yu,Aixi

doi:10.3892/etm.2014.1567

Vacuum‑assisted closure increases ICAM‑1, MIF, VEGF and collagen I expression in wound therapy

Authors:
- Weiyang Wang
- Zhenyu Pan
- Xiang Hu
- Zonghuan Li
- Yong Zhao
- Aixi Yu
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Affiliations: Department of Micro‑Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China
Published online on: February 20, 2014 https://doi.org/10.3892/etm.2014.1567
Pages: 1221-1226

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Abstract

Severe traumatic wounds are challenging to manage during surgery. The introduction of vacuum‑assisted closure (VAC) is a breakthrough in wound management. The aim of the present study was to investigate the effect of VAC on cytokines in wounds during the management of severe traumatic wounds following initial debridement. VAC and conventional wound care (CWC) were independently applied to severe traumatic wounds on pigs. The expression levels of intercellular adhesion molecule‑1 (ICAM‑1), migration inhibitory factor (MIF), vascular endothelial growth factor (VEGF), basic fibroblast growth factor, collagen I and human fibroblast collagenase 1 were detected by quantitative polymerase chain reaction and western blotting. VAC significantly increased the expression of ICAM‑1, MIF, VEGF and collagen I compared with that induced by CWC at the protein and mRNA levels. Therefore, the results of the present study indicate that VAC therapy is an effective method for treating severe traumatic wounds, as it increases the expression of cytokines in wounds. VAC significantly increases the expression of ICAM‑1, MIF, VEGF and collagen I to manage severe traumatic wounds.

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