The expression of renal Epstein‑Barr virus markers in patients with lupus nephritis

Yu,Xiao‑Xia; Yao,Cui‑Wei; Tao,Jing‑Li; Yang,Chen; Luo,Mian‑Na; Li,Shang‑Mei; Liu,Hua‑Feng

doi:10.3892/etm.2014.1578

May-2014 Volume 7 Issue 5

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

May-2014 Volume 7 Issue 5

Full Size Image

Article

The expression of renal Epstein‑Barr virus markers in patients with lupus nephritis

Authors:
- Xiao‑Xia Yu
- Cui‑Wei Yao
- Jing‑Li Tao
- Chen Yang
- Mian‑Na Luo
- Shang‑Mei Li
- Hua‑Feng Liu
View Affiliations / Copyright

Affiliations: Institute of Nephrology, Guangdong Medical College, Zhanjiang, Guangdong 524001, P.R. China
Pages: 1135-1140
|
Published online on: February 24, 2014

https://doi.org/10.3892/etm.2014.1578
Expand metrics +

Abstract

The aim of this study was to investigate the role of renal Epstein‑Barr virus (EBV) infection in the pathogenesis of lupus nephritis (LN). A total of 58 renal tissue samples from patients with LN, seven normal renal tissue samples from patients with non‑glomerular hematuria and 37 renal tissue samples from patients with minimal change nephropathy were collected. The expression of EBV‑latent membrane protein‑1 (EBV‑LMP1) and EBV‑encoded RNA 1 (EBER-1) in the renal tissue was examined by immunohistochemistry (IHC) and in situ hybridization (ISH), respectively. The sera levels of anti-nuclear antibody as well as antibodies to extractable nuclear antigen in patients with LN were also measured. An equivalence test showed that the results from the IHC and the ISH analyses had strong agreement. The positive rates of renal EBER-1 and EBV‑LMP1 in the LN patients were significantly higher than those of the normal and minimal change nephropathy patients (P<0.001), while no significant difference was identified between those of the normal and minimal change nephropathy groups (P>0.05). The positive rates of EBV‑LMP1 and EBER-1 in the renal tissues of patients with LN were not determined to be significantly different between the relapse (immunosuppressant‑treated) and initial onset (non‑treated) patients, between the patients with and without concurrent infection, and among the patients with different age ranges (P>0.05). The proportion of LN patients positive for anti‑Sm antibody was significantly higher in the renal EBV‑positive group than in the EBV‑negative group (P<0.05), while the proportions of LN patients positive for the other autoantibodies that were examined were not identified to be significantly different between these two groups (P>0.05). The present study shows that renal EBV infection may contribute to the pathogenesis of LN by inducing anti‑Sm antibody production.

View Figures

View References

1	Simard JF and Costenbader KH: What can epidemiology tell us about systemic lupus erythematosus? Int J Clin Pract. 61:1170–1180. 2007. View Article : Google Scholar : PubMed/NCBI
2	Pérez-Mercado AE and Vilá-Pérez S: Cytomegalovirus as a trigger for systemic lupus erythematosus. J Clin Rheumatol. 16:335–337. 2010.PubMed/NCBI
3	Pavlovic M, Kats A, Cavallo M and Shoenfeld Y: Clinical and molecular evidence for association of SLE with parvovirus B19. Lupus. 19:783–792. 2010. View Article : Google Scholar : PubMed/NCBI
4	Sun Y, Sun S, Li W, Li B and Li J: Prevalence of human herpesvirus 8 infection in systemic lupus erythematosus. Virol J. 8:2102011. View Article : Google Scholar : PubMed/NCBI
5	Barzilai O, Ram M and Shoenfeld Y: Viral infection can induce the production of autoantibodies. Curr Opin Rheumatol. 19:636–643. 2007. View Article : Google Scholar : PubMed/NCBI
6	Evans AS, Rothfield NF and Niederman JC: Raised antibody titres to E.B. virus in systemic lupus erythematosus. Lancet. 297:167–168. 1971. View Article : Google Scholar : PubMed/NCBI
7	James JA, Kaufman KM, Farris AD, Taylor-Albert E, Lehman TJ and Harley JB: An increased prevalence of Epstein-Barr virus infection in young patients suggests a possible etiology for systemic lupus erythematosus. J Clin Invest. 100:3019–3026. 1997. View Article : Google Scholar
8	James JA, Neas BR, Moser KL, et al: Systemic lupus erythematosus in adults is associated with previous Epstein-Barr virus exposure. Arthritis Rheum. 44:1122–1126. 2001. View Article : Google Scholar : PubMed/NCBI
9	Moon UY, Park SJ, Oh ST, et al: Patients with systemic lupus erythematosus have abnormally elevated Epstein-Barr virus load in blood. Arthritis Res Ther. 6:R295–R302. 2004. View Article : Google Scholar : PubMed/NCBI
10	Chen CJ, Lin KH, Lin SC, et al: High prevalence of immunoglobulin A antibody against Epstein-Barr virus capsid antigen in adult patients with lupus with disease flare: case control studies. J Rheumatol. 32:44–47. 2005.PubMed/NCBI
11	Yu SF, Wu HC, Tsai WC, et al: Detecting Epstein-Barr virus DNA from peripheral blood mononuclear cells in adult patients with systemic lupus erythematosus in Taiwan. Med Microbiol Immunol. 194:115–120. 2005. View Article : Google Scholar : PubMed/NCBI
12	Lu JJ, Chen DY, Hsieh CW, Lan JL, Lin FJ and Lin SH: Association of Epstein-Barr virus infection with systemic lupus erythematosus in Taiwan. Lupus. 16:168–175. 2007.PubMed/NCBI
13	Poole BD, Templeton AK, Guthridge JM, Brown EJ, Harley JB and James JA: Aberrant Epstein-Barr viral infection in systemic lupus erythematosus. Autoimmun Rev. 8:337–342. 2009. View Article : Google Scholar : PubMed/NCBI
14	Hochberg MC: Updating the American College of Rheumatology revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 40:17251997. View Article : Google Scholar : PubMed/NCBI
15	Cao SM, Simons MJ and Qian CN: The prevalence and prevention of nasopharyngeal carcinoma in China. Chin J Cancer. 30:114–119. 2011. View Article : Google Scholar : PubMed/NCBI
16	Odumade OA, Hogquist KA and Balfour HH Jr: Progress and problems in understanding and managing primary Epstein-Barr virus infections. Clin Microbiol Rev. 24:193–209. 2011. View Article : Google Scholar : PubMed/NCBI
17	Vetsika EK and Callan M: Infectious mononucleosis and Epstein-Barr virus. Expert Rev Mol Med. 6:1–16. 2004. View Article : Google Scholar : PubMed/NCBI
18	Okano M: Overview and problematic standpoints of severe chronic active Epstein-Barr virus infection syndrome. Crit Rev Oncol Hematol. 44:273–282. 2002. View Article : Google Scholar : PubMed/NCBI
19	Lerner MR, Andrews NC, Miller G and Steitz JA: Two small RNAs encoded by Epstein-Barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus. Proc Natl Acad Sci USA. 78:805–809. 1981. View Article : Google Scholar : PubMed/NCBI
20	Gulley ML: Molecular diagnosis of Epstein-Barr virus-related diseases. J Mol Diagn. 3:1–10. 2001. View Article : Google Scholar : PubMed/NCBI
21	Harley JB and James JA: Autoepitopes in lupus. J Lab Clin Med. 126:509–516. 1995.
22	Kaufman KM, Kirby MY, Harley JB and James JA: Peptide mimics of a major lupus epitope of SmB/B′. Ann NY Acad Sci. 987:215–229. 2003.PubMed/NCBI
23	Poole BD, Gross T, Maier S, Harley JB and James JA: Lupus-like autoantibody development in rabbits and mice after immunization with EBNA-1 fragments. J Autoimmun. 31:362–371. 2008. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

The expression of renal Epstein‑Barr virus markers in patients with lupus nephritis

This article is mentioned in:

Abstract

Figure 1

Related Articles