PinX1‑siRNA/mPEG‑PEI‑SPION combined with doxorubicin enhances the inhibition of glioma growth

  • Authors:
    • Ling Long
    • Weiwei Wang
    • Xia‑Dong Cai
    • Du Cheng
    • Xintao Shuai
    • Ying Peng
  • View Affiliations

  • Published online on: February 26, 2014     https://doi.org/10.3892/etm.2014.1586
  • Pages: 1170-1176
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Resistance to chemotherapy and the side effects of anticancer drugs are the major obstacles for glioma treatment. The aim of the present study was to develop a novel approach for the treatment of gliomas that improved the therapeutic effect; the anticancer drug, doxorubicin (DOX), was combined with short interfering (si)RNA and monomethoxy polyethylene glycol polyethylenimine superparamagnetic iron oxide nanoparticle (mPEG‑PEI‑SPION), a magnetic resonance imaging (MRI)‑visible nanoparticle. Specific siRNA molecules, delivered by mPEG‑PEI‑SPION, were employed to knockdown the PIN2‑interacting protein 1 (PinX1) gene in C6 glioma cells. PinX1 is a nucleolar protein associated with telomere and telomerase. C6 cells were treated with DOX and/or PinX1‑siRNA. The results of the transfection experiments revealed that siRNA/mPEG‑PEI‑SPION was transfected into C6 cells with high efficiency. PinX1‑siRNA was unable to inhibit C6 cells, while in the PinX1‑siRNA + DOX group, the same dose of DOX caused an increased loss of cell viability. Therefore, mPEG‑PEI‑SPION was shown to be viable for siRNA delivery into C6 cells and coadministration of DOX with PinX1‑siRNA may be a potential therapeutic method for inhibiting gliomas.
View Figures
View References

Related Articles

Journal Cover

May-2014
Volume 7 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Long L, Wang W, Cai XD, Cheng D, Shuai X and Peng Y: PinX1‑siRNA/mPEG‑PEI‑SPION combined with doxorubicin enhances the inhibition of glioma growth. Exp Ther Med 7: 1170-1176, 2014
APA
Long, L., Wang, W., Cai, X., Cheng, D., Shuai, X., & Peng, Y. (2014). PinX1‑siRNA/mPEG‑PEI‑SPION combined with doxorubicin enhances the inhibition of glioma growth. Experimental and Therapeutic Medicine, 7, 1170-1176. https://doi.org/10.3892/etm.2014.1586
MLA
Long, L., Wang, W., Cai, X., Cheng, D., Shuai, X., Peng, Y."PinX1‑siRNA/mPEG‑PEI‑SPION combined with doxorubicin enhances the inhibition of glioma growth". Experimental and Therapeutic Medicine 7.5 (2014): 1170-1176.
Chicago
Long, L., Wang, W., Cai, X., Cheng, D., Shuai, X., Peng, Y."PinX1‑siRNA/mPEG‑PEI‑SPION combined with doxorubicin enhances the inhibition of glioma growth". Experimental and Therapeutic Medicine 7, no. 5 (2014): 1170-1176. https://doi.org/10.3892/etm.2014.1586