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Article

PinX1‑siRNA/mPEG‑PEI‑SPION combined with doxorubicin enhances the inhibition of glioma growth

  • Authors:
    • Ling Long
    • Weiwei Wang
    • Xia‑Dong Cai
    • Du Cheng
    • Xintao Shuai
    • Ying Peng
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, The Third Affiliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510630, P.R. China, Center of Biomedical Engineering, School of Chemistry and Chemical Engineering, Sun Yat‑Sen University, Guangzhou, Guangdong 510275, P.R. China, Department of Neurology, The Sixth Affiliated Hospital (Guangdong Gastrointestinal and Anal Hospital), Sun Yat‑Sen University, Guangzhou, Guangdong 510655, P.R. China, Department of Neurology, Sun Yat‑Sen Memorial Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510120, P.R. China
  • Pages: 1170-1176
    |
    Published online on: February 26, 2014
       https://doi.org/10.3892/etm.2014.1586
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Abstract

Resistance to chemotherapy and the side effects of anticancer drugs are the major obstacles for glioma treatment. The aim of the present study was to develop a novel approach for the treatment of gliomas that improved the therapeutic effect; the anticancer drug, doxorubicin (DOX), was combined with short interfering (si)RNA and monomethoxy polyethylene glycol polyethylenimine superparamagnetic iron oxide nanoparticle (mPEG‑PEI‑SPION), a magnetic resonance imaging (MRI)‑visible nanoparticle. Specific siRNA molecules, delivered by mPEG‑PEI‑SPION, were employed to knockdown the PIN2‑interacting protein 1 (PinX1) gene in C6 glioma cells. PinX1 is a nucleolar protein associated with telomere and telomerase. C6 cells were treated with DOX and/or PinX1‑siRNA. The results of the transfection experiments revealed that siRNA/mPEG‑PEI‑SPION was transfected into C6 cells with high efficiency. PinX1‑siRNA was unable to inhibit C6 cells, while in the PinX1‑siRNA + DOX group, the same dose of DOX caused an increased loss of cell viability. Therefore, mPEG‑PEI‑SPION was shown to be viable for siRNA delivery into C6 cells and coadministration of DOX with PinX1‑siRNA may be a potential therapeutic method for inhibiting gliomas.
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Copy and paste a formatted citation
Spandidos Publications style
Long L, Wang W, Cai XD, Cheng D, Shuai X and Peng Y: PinX1‑siRNA/mPEG‑PEI‑SPION combined with doxorubicin enhances the inhibition of glioma growth. Exp Ther Med 7: 1170-1176, 2014.
APA
Long, L., Wang, W., Cai, X., Cheng, D., Shuai, X., & Peng, Y. (2014). PinX1‑siRNA/mPEG‑PEI‑SPION combined with doxorubicin enhances the inhibition of glioma growth. Experimental and Therapeutic Medicine, 7, 1170-1176. https://doi.org/10.3892/etm.2014.1586
MLA
Long, L., Wang, W., Cai, X., Cheng, D., Shuai, X., Peng, Y."PinX1‑siRNA/mPEG‑PEI‑SPION combined with doxorubicin enhances the inhibition of glioma growth". Experimental and Therapeutic Medicine 7.5 (2014): 1170-1176.
Chicago
Long, L., Wang, W., Cai, X., Cheng, D., Shuai, X., Peng, Y."PinX1‑siRNA/mPEG‑PEI‑SPION combined with doxorubicin enhances the inhibition of glioma growth". Experimental and Therapeutic Medicine 7, no. 5 (2014): 1170-1176. https://doi.org/10.3892/etm.2014.1586
Copy and paste a formatted citation
x
Spandidos Publications style
Long L, Wang W, Cai XD, Cheng D, Shuai X and Peng Y: PinX1‑siRNA/mPEG‑PEI‑SPION combined with doxorubicin enhances the inhibition of glioma growth. Exp Ther Med 7: 1170-1176, 2014.
APA
Long, L., Wang, W., Cai, X., Cheng, D., Shuai, X., & Peng, Y. (2014). PinX1‑siRNA/mPEG‑PEI‑SPION combined with doxorubicin enhances the inhibition of glioma growth. Experimental and Therapeutic Medicine, 7, 1170-1176. https://doi.org/10.3892/etm.2014.1586
MLA
Long, L., Wang, W., Cai, X., Cheng, D., Shuai, X., Peng, Y."PinX1‑siRNA/mPEG‑PEI‑SPION combined with doxorubicin enhances the inhibition of glioma growth". Experimental and Therapeutic Medicine 7.5 (2014): 1170-1176.
Chicago
Long, L., Wang, W., Cai, X., Cheng, D., Shuai, X., Peng, Y."PinX1‑siRNA/mPEG‑PEI‑SPION combined with doxorubicin enhances the inhibition of glioma growth". Experimental and Therapeutic Medicine 7, no. 5 (2014): 1170-1176. https://doi.org/10.3892/etm.2014.1586
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