Effect of γ‑tocotrienol in counteracting oxidative stress and joint damage in collagen‑induced arthritis in rats

Radhakrishnan,Ammu; Tudawe,Dulanthi; Chakravarthi,Srikumar; Chiew,Gan Seng; Haleagrahara,Nagaraja

doi:10.3892/etm.2014.1592

Effect of γ‑tocotrienol in counteracting oxidative stress and joint damage in collagen‑induced arthritis in rats

Authors:
- Ammu Radhakrishnan
- Dulanthi Tudawe
- Srikumar Chakravarthi
- Gan Seng Chiew
- Nagaraja Haleagrahara
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Affiliations: Faculty of Medicine and Health Sciences, International Medical University, Kuala Lumpur 57000, Malaysia, Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, Kuala Lumpur 57000, Malaysia, Discipline of Physiology and Pharmacology, School of Veterinary and Biomedical Sciences, James Cook University, Townsville, Queensland 4811, Australia
Published online on: February 28, 2014 https://doi.org/10.3892/etm.2014.1592
Pages: 1408-1414

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Abstract

Tocotrienols exhibit a significant anti‑inflammatory and antioxidant effect in numerous human diseases. However, the anti‑inflammatory and antioxidant effects of tocotrienols in arthritic conditions are not well documented. Therefore, the effect of γ‑tocotrienol supplementation against oxidative stress and joint pathology in collagen-induced arthritis in rats was investigated in the present study. Adult female Dark Agouti rats were randomly divided into groups: Control, γ‑tocotrienol alone, arthritis alone and arthritis with γ‑tocotrienol. Arthritis was induced using 4 mg/kg body weight collagen in complete Freund's adjuvant. The rats were treated orally with 5 mg/kg body weight of γ‑tocotrienol between day 21 and day 45. After 45 days, serum C‑reactive protein (CRP), tumor necrosis factor (TNF)‑α, superoxide dismutase (SOD) and total glutathione (GSH) assays were conducted. γ‑tocotrienol significantly reduced the arthritis-induced changes in body weight, CRP, TNF‑α, SOD and the total GSH levels. There was a significant reduction in the arthritis‑induced histopathological changes in the γ‑tocotrienol treatment group. The data indicated that administration of γ‑tocotrienol resulted in a significant antioxidant and anti-inflammatory effect on collagen-induced arthritis; therefore, γ‑tocotrienol may have therapeutic potential as a long‑term anti‑arthritic agent in rheumatoid arthritis therapy.

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