Contrast‑enhanced ultrasound analysis of tissue perfusion in tumor-bearing mice following treatment with endostatin combined with radiotherapy

Ge,Wei; Zheng,Yongfa; Tao,Zezhang

doi:10.3892/etm.2014.1594

Contrast‑enhanced ultrasound analysis of tissue perfusion in tumor-bearing mice following treatment with endostatin combined with radiotherapy

Authors:
- Wei Ge
- Yongfa Zheng
- Zezhang Tao
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Affiliations: Department of Oncology, Renmin Hospital, Wuhan University, Wuhan, Hubei 430060, P.R. China, Department of Otolaryngology, Renmin Hospital, Wuhan University, Wuhan, Hubei 430060, P.R. China
Published online on: February 28, 2014 https://doi.org/10.3892/etm.2014.1594
Pages: 1359-1363

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Abstract

The aim of this study was to observe the effects of Endostar (recombinant human endostatin) and radiotherapy, singly or in combination, on blood flow in mouse tumour tissue using ultrasound imaging. The ultrasound contrast agent, SonoVue, was used for the contrast‑enhanced ultrasound examinations. SonoLiver software was used to analyse dynamic vascular patterns (DVPs) in the contrast process. Blood perfusion data were collected and statistical analysis was performed for data processing. Results were presented as DVP curves and quantitative parameters. Quantitative parameters showed statistically significant (P<0.05) differences in peak strength, rise time, time to peak and mean transit time among the various treatment groups. Changes in tumour blood perfusion were quantified by the assessment of contrast‑enhanced ultrasound parameters. The results indirectly reflected the degree of change in angiogenesis in the tumour following experimental intervention. Ultrasound contrast imaging effectively showed the extent of the changes in vascularity and flow state. Therefore, contrast‑enhanced ultrasound is suitable for use as an indicator of blood flow changes in an experimental model.

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