Renal neutrophil gelatinase‑associated lipocalin and kidney injury molecule‑1 expression in children with acute kidney injury and Henoch‑Schönlein purpura nephritis

Du,Yue; Hou,Ling; Guo,Jinjie; Sun,Tingting; Wang,Xiuli; Wu,Yubin

doi:10.3892/etm.2014.1595

Renal neutrophil gelatinase‑associated lipocalin and kidney injury molecule‑1 expression in children with acute kidney injury and Henoch‑Schönlein purpura nephritis

Authors:
- Yue Du
- Ling Hou
- Jinjie Guo
- Tingting Sun
- Xiuli Wang
- Yubin Wu
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Affiliations: Department of Pediatric Nephrology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China
Published online on: February 28, 2014 https://doi.org/10.3892/etm.2014.1595
Pages: 1130-1134

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Abstract

The aim of this study was to investigate the expression of neutrophil gelatinase‑associated lipocalin (NGAL) and kidney injury molecule‑1 (KIM‑1) in the serum, urine and renal tissues of children with acute kidney injury (AKI) and Henoch‑Schönlein purpura nephritis (A‑on‑C). A prospective single‑center evaluation of the serum, urine and renal NGAL and KIM‑1 levels was performed in a cohort of children. Blood and 5‑ml urine samples were collected from each patient for the analysis of NGAL and KIM‑1 levels using an ELISA. In addition, the expression of NGAL and KIM‑1 in the kidney was examined using immunohistochemistry in patients with A‑on‑C and HSPN. The expression of serum cystatin C, β2‑macroglobulin and serum creatinine (SCr), as well as urinary β2‑MG and SCr, in the patients with A‑on‑C was significantly higher than that of HSPN patients, and the expression of NGAL and KIM‑1 in the serum and urine in the A‑on‑C patients was also significantly higher than that of HSPN patients. However, there were no significant differences in the urine protein levels between the two groups. NGAL and KIM‑1 were expressed in renal tubular epithelial cells, and the expression of NGAL and KIM‑1 in the A‑on‑C patients was significantly higher than that in HSPN patients. In addition, the urine NGAL and KIM‑1 levels were negatively correlated with glomerular filtration rate, but there was no significant correlation between the urine NGAL/KIM‑1 and urine protein levels. The changes in serum and urine NGAL and KIM‑1 levels may be applied to the diagnosis of A‑on‑C.

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