Apoptosis of human gastric cancer SGC‑7901 cells induced by podophyllotoxin

Ji,Chen‑Feng; Ji,Yu‑Bin

doi:10.3892/etm.2014.1606

May-2014 Volume 7 Issue 5

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May-2014 Volume 7 Issue 5

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Article

Apoptosis of human gastric cancer SGC‑7901 cells induced by podophyllotoxin

Authors:
- Chen‑Feng Ji
- Yu‑Bin Ji
View Affiliations / Copyright

Affiliations: Engineering Research Center of Natural Anticancer Drugs, Ministry of Education, Harbin, Heilongjiang 150076, P.R. China
Pages: 1317-1322
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Published online on: March 6, 2014

https://doi.org/10.3892/etm.2014.1606
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Abstract

Numerous studies have demonstrated that podophyllotoxin and its derivatives exhibit antitumor effects. The aim of the present study was to investigate SGC‑7901 cell apoptosis and the underlying mechanism induced by podophyllotoxin. SGC‑7901 cells were treated with varying concentrations of podophyllotoxin. MTT assays and flow cytometry were used to evaluate the effects of podophyllotoxin on the proliferation and apoptosis of SGC‑7901 cells, while fluorescence inverted microscopy was used to observe the morphology of SGC‑7901 cells that had been dyed with Hoechst 33258. In addition, laser scanning confocal microscopy was used to analyze the mitochondrial membrane potential (MMP) of SGC‑7901 cells dyed with Rhodamine 123. Western blotting was performed to analyze the expression levels of cytochrome c (cyt‑c), caspase‑9 and caspase‑3 in the SGC‑7901 cells. The results indicated that podophyllotoxin was capable of inhibiting growth and inducing the apoptosis of SGC‑7901 cells in a dose‑dependent manner, causing cell cycle arrest at the G2/M phase. After 48 h of treatment, the apoptotic morphology of SGC‑7901 cells was clear, exhibiting cell protuberance, concentrated cytoplasms and apoptotic bodies. Following 24 h of treatment, the MMP of the SGC‑7901 cells decreased. In addition, after 48 h, the expression of cyt‑c was shown to be upregulated, while the expression levels of pro‑caspase‑9 and pro‑caspase‑3 in the SGC‑7901 cells were shown to be downregulated. In conclusion, apoptosis can be induced in SGC‑7901 cells by podophyllotoxin, potentially via a mitochondrial pathway, indicating that podophyllotoxin may be a potent agent for cancer treatment.

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1	Hu Y, Fang JY and Xiao SD: Can the incidence of gastric cancer be reduced in the new century? J Dig Dis. 14:11–15. 2013. View Article : Google Scholar : PubMed/NCBI
2	Ji YB, Ji CF and Zhang H: Laminarin induces apoptosis of human colon cancer LOVO cells through a mitochondrial pathway. Molecules. 17:9947–9960. 2012. View Article : Google Scholar : PubMed/NCBI
3	Ji YB, Ji CF, Yue L and Xu H: Saponins isolated from Asparagus induce apoptosis in human hepatoma cell line HepG2 through a mitochondrial-mediated pathway. Curr Oncol. 19(Suppl 2): eS1–eS9. 2012.PubMed/NCBI
4	Wang B, Chen L, Zhen H, et al: Proteomic changes induced by podophyllotoxin in human cervical carcinoma HeLa cells. Am J Chin Med. 41:163–175. 2013. View Article : Google Scholar : PubMed/NCBI
5	Yang TM, Qi SN, Zhao N, et al: Induction of apoptosis through caspase-independent or caspase-9-dependent pathway in mouse and human osteosarcoma cells by a new nitroxyl spin-labeled derivative of podophyllotoxin. Apoptosis. 18:727–738. 2013. View Article : Google Scholar : PubMed/NCBI
6	Rojas-Sepúlveda AM, Mendieta-Serrana M, Mojica MY, et al: Cytotoxic podophyllotoxin type-lignans from the steam bark of Bursera fagaroides var. fagaroides. Molecules. 17:9506–9519. 2012.PubMed/NCBI
7	Xu H, Lv M and Tian X: A review on hemisynthesis, biosynthesis, biological activities, mode of action, and structure-activity relationship of podophyllotoxins: 2003–2007. Curr Med Chem. 16:327–349. 2009.PubMed/NCBI
8	Hartmann JT and Lipp HP: Camptothecin and podophyllotoxin derivatives: inhibitors of topoisomerase I and II - mechanisms of action, pharmacokinetics and toxicity profile. Drug Saf. 29:209–230. 2006. View Article : Google Scholar : PubMed/NCBI
9	Zhang J, Zhou CS, Liu S, Chen H and Yang C: Effect of podophyllotoxin on human gastric cancer cell line SGC 7901. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 33:718–722. 2008.(In Chinese).
10	Martin LJ: The mitochondrial permeability transition pore: a molecular target for amyotrophic lateral sclerosis therapy. Biochim Biophys Acta. 1802:186–197. 2010. View Article : Google Scholar : PubMed/NCBI
11	Lemeshko VV: Potential-dependent membrane permeabilization and mitochondrial aggregation caused by anticancer polyarginine-KLA peptides. Arch Biochem Biophys. 493:213–220. 2010. View Article : Google Scholar : PubMed/NCBI
12	Scorrano L: Opening the doors to cytochrome c: changes in mitochondrial shape and apoptosis. Int J Biochem Cell Biol. 41:1875–1883. 2010. View Article : Google Scholar : PubMed/NCBI
13	dos Santos AB, Dorta DJ, Pestana CR, et al: Dehydromonocrotaline induces cyclosporine A-insensitive mitochondrial permeability transition/cytochrome c release. Toxicon. 54:16–22. 2009.PubMed/NCBI
14	Lee HJ, Lee HJ, Lee EO, et al: Mitochondria-cytochrome c caspase-9 cascade mediates isorhamnetin-induced apoptosis. Cancer Lett. 270:342–353. 2008. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Apoptosis of human gastric cancer SGC‑7901 cells induced by podophyllotoxin

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