Monoamine neurotransmitters and fibroblast growth factor‑2 in the brains of rats with post‑stroke depression

Ji,Xiao‑Wei; Wu,Chun‑Ling; Wang,Xing‑Chen; Liu,Jie; Bi,Jian‑Zhong; Wang,Dian‑Yun

doi:10.3892/etm.2014.1674

Monoamine neurotransmitters and fibroblast growth factor‑2 in the brains of rats with post‑stroke depression

Authors:
- Xiao‑Wei Ji
- Chun‑Ling Wu
- Xing‑Chen Wang
- Jie Liu
- Jian‑Zhong Bi
- Dian‑Yun Wang
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Affiliations: Department of Neurology, Second Hospital Affiliated to Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250001, P.R. China, Blood Purification Center, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250013, P.R. China, Department of Neurology, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China, Department of Traditional Chinese Medicine, Dongying People's Hospital, Dongying, Shandong 257091, P.R. China
Published online on: April 8, 2014 https://doi.org/10.3892/etm.2014.1674
Pages: 159-164

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Abstract

The aim of the present study was to investigate the changes in the levels of serotonin (5‑HT), dopamine (DA), norepinephrine (NE) and fibroblast growth factor‑2 (FGF‑2) in the brains of rats with post‑stroke depression (PSD). A rat model of stroke was established by middle cerebral artery occlusion and the rats were randomly divided into two groups: Control and modification groups. The rats in the modification group had PSD, while the rats in the control group had experienced a stroke only. The PSD model was established by applying chronic mild stress to the individually housed rats. High‑performance liquid chromatography was used to detect the levels of 5‑HT, DA and NE, while western blotting was used to detect the FGF‑2 protein expression levels in the frontal lobe and hippocampus. Quantitative polymerase chain reaction was also used to determine the mRNA expression levels of FGF‑2 in the frontal lobes of the two groups. The levels of 5‑HT, DA and NE in the frontal lobe and hippocampus of the rats in the PSD group were significantly lower than the levels observed in the rats in the stroke group (P<0.01). In addition, protein expression levels of FGF‑2 in the frontal lobe of the rats in the PSD group were significantly lower when compared with the control group rats (P<0.01), however, the protein expression levels of FGF‑2 in the hippocampus did not exhibit a statistically significant difference (P>0.05). The mRNA expression levels of FGF‑2 in the frontal lobe of the rats in the modification group were significantly lower than the levels in the control group rats (P<0.01). Therefore, reduced levels of monoamine neurotransmitters and FGF‑2 expression in the brains of rats with PSD are associated with the incidence of PSD.

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1	Yang LL, Zhang ZJ and Sun DM: Incidence and its risk factors of post-stroke depression in cerebral stroke patients at acute stage. Lin Chuang Shen Jing Bing Xue Za Zhi. 23:185–187. 2010.(In Chinese).
2	Berg C, Backström T, Winberg S, et al: Developmental exposure to fluoxetine modulates the serotonin system in hypothalamus. PLoS One. 8:e550532013. View Article : Google Scholar : PubMed/NCBI
3	Sullivan GM, Ogden RT, Huang YY, et al: Higher in vivo serotonin-1a binding in posttraumatic stress disorder: a PET study with [11C]WAY-100635. Depress Anxiety. 30:197–206. 2013.PubMed/NCBI
4	Spasojevic N, Jovanovic P and Dronjak S: Chronic fluoxetine treatment affects gene expression of catecholamine enzymes in the heart of depression model rats. Indian J Exp Biol. 50:771–775. 2012.PubMed/NCBI
5	Vergaño-Vera E, Méndez-Gómez HR, Hurtado-Chong A, et al: Fibroblast growth factor-2 increases the expression of neurogenic genes and promotes the migration and differentiation of neurons derived from transplanted neural stem/progenitor cells. Neuroscience. 162:39–54. 2009.PubMed/NCBI
6	Reynolds BA and Weiss S: Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science. 255:1707–1710. 1992. View Article : Google Scholar : PubMed/NCBI
7	Vicario-Abejón C, Yusta-Boyo MJ, Fernández-Moreno C and de Pablo F: Locally born olfactory bulb stem cells proliferate in response to insulin-related factors and require endogenous insulin-like growth factor-I for differentiation into neurons and glia. J Neurosci. 23:895–906. 2003.PubMed/NCBI
8	Gritti A, Parati EA, Cova L, Frolichsthal P, et al: Multipotential stem cells from the adult mouse brain proliferate and self-renew in response to basic fibroblast growth factor. J Neurosci. 16:1091–1100. 1996.PubMed/NCBI
9	Turner CA, Watson SJ and Akil H: The fibroblast growth factor family: neuromodulation of affective behavior. Neuron. 76:160–174. 2012. View Article : Google Scholar : PubMed/NCBI
10	Fukuchi-Shimogori T and Grove EA: Neocortex patterning by the secreted signaling molecule FGF8. Science. 294:1071–1074. 2001. View Article : Google Scholar : PubMed/NCBI
11	Shin DM, Korada S, Raballo R, et al: Loss of glutamatergic pyramidal neurons in frontal and temporal cortex resulting from attenuation of FGFR1 signaling is associated with spontaneous hyperactivity in mice. J Neurosci. 24:2247–2258. 2004. View Article : Google Scholar : PubMed/NCBI
12	Guillemot F and Zimmer C: From cradle to grave: the multiple roles of fibroblast growth factors in neural development. Neuron. 71:574–588. 2011. View Article : Google Scholar : PubMed/NCBI
13	Vaccarino FM, Schwartz ML, Raballo R, et al: Changes in cerebral cortex size are governed by fibroblast growth factor during embryogenesis. Nat Neurosci. 2:246–253. 1999. View Article : Google Scholar
14	Raballo R, Rhee J, Lyn-Cook R, et al: Basic fibroblast growth factor (Fgf2) is necessary for cell proliferation and neurogenesis in the developing cerebral cortex. J Neurosci. 20:5012–5023
15	Mason I: Initiation to end point: the multiple roles of fibroblast growth factors in neural development. Nat Rev Neurosci. 8:583–596. 2007. View Article : Google Scholar : PubMed/NCBI
16	Ciccolini F and Svendsen CN: Fibroblast growth factor 2 (FGF-2) promotes acquisition of epidermal growth factor (EGF) responsiveness in mouse striatal precursor cells: identification of neural precursors responding to both EGF and FGF-2. J Neurosci. 18:7869–7880. 1998.
17	Ito Y, Tsurushima H, Sato M, et al: Angiogenesis therapy for brain infarction using a slow-releasing drug delivery system for fibroblast growth factor 2. Biochem Biophys Res Commun. 432:182–187. 2013. View Article : Google Scholar : PubMed/NCBI
18	Maric D, Fiorio Pla A, Chang YH and Barker JL: Self-renewing and differentiating properties of cortical neural stem cells are selectively regulated by basic fibroblast growth factor (FGF) signaling via specific FGF receptors. J Neurosci. 27:1836–1852. 2007. View Article : Google Scholar
19	Sun Y, Hu J, Zhou L, et al: Interplay between FGF2 and BMP controls the self-renewal, dormancy and differentiation of rat neural stem cells. J Cell Sci. 124:1867–1877. 2011. View Article : Google Scholar : PubMed/NCBI
20	Vicario-Abejón C, Johe KK, Hazel TG, et al: Functions of basic fibroblast growth factor and neurotrophins in the differentiation of hippocampal neurons. Neuron. 15:105–114. 1995.PubMed/NCBI
21	Cattaneo E and McKay R: Proliferation and differentiation of neuronal stem cells regulated by nerve growth factor. Nature. 347:762–765. 1990. View Article : Google Scholar : PubMed/NCBI
22	Gillen R, Tennen H, McKee TE, et al: Depressive symptoms and history of depression predict rehabilitation efficiency in stroke patients. Arch Phys Med Rehabil. 82:1645–1649. 2001. View Article : Google Scholar : PubMed/NCBI
23	Singh A, Black SE, Herrmann N, et al: Functional and neuroanatomic correlations in poststroke depression: the Sunnybrook Stroke Study. Stroke. 31:637–644. 2000. View Article : Google Scholar : PubMed/NCBI
24	Vataja R, Pohjasvaara T, Mäntylä R, et al: Depression-executive dysfunction syndrome in stroke patients. Am J Geriatr Psychiatry. 13:99–107. 2005.PubMed/NCBI
25	Kishi Y, Kosier T and Robinson RG: Suicidal plans in patients with acute stroke. J Nerv Ment Dis. 184:274–280. 1996. View Article : Google Scholar : PubMed/NCBI
26	House A, Knapp E, Bamford J and Vail A: Mortality at 12 and 24 months after stroke may be associated with depressive symptoms at 1 month. Stroke. 32:696–701. 2001. View Article : Google Scholar : PubMed/NCBI
27	Everson SA, Roberts RE, Goldberg DE and Kaplan GA: Depressive symptoms and increased risk of stroke mortality over a 29-year period. Arch Intern Med. 158:1133–1138. 1998.PubMed/NCBI
28	Robinson RG, Bolduc PL and Price TR: Two-year longitudinal study of poststroke mood disorders:diagnosis and outcome at one and two years. Stroke. 18:837–843. 1987.PubMed/NCBI
29	Simonsick EM, Wallace RB, Blazer DG and Berkman LF: Depressive symptomatology and hypertension-associated morbidity and mortality in older adults. Psychosom Med. 57:427–435. 1995. View Article : Google Scholar : PubMed/NCBI
30	Vescovi AL, Reynolds BA, Fraser DD and Weiss S: bFGF regulates the proliferative fate of unipotent (neuronal) and bipotent (neuronal/astroglial) EGF-generated CNS progenitor cells. Neuron. 11:951–966. 1993. View Article : Google Scholar : PubMed/NCBI
31	Lukaszewicz A, Savatier P, Cortay V, et al: Contrasting effects of basic fibroblast growth factor and neurotrophin 3 on cell cycle kinetics of mouse cortical stem cells. J Neurosci. 22:6610–6622. 2002.PubMed/NCBI
32	Lillien L and Raphael H: BMP and FGF regulate the development of EGF-responsive neural progenitor cells. Development. 127:4993–5005. 2000.PubMed/NCBI
33	Ikeda N, Nonoguchi N, Zhao MZ, et al: Bone marrow stromal cells that enhanced fibroblast growth factor-2 secretion by herpes simplex virus vector improve neurological outcome after transient focal cerebral ischemia in rats. Stroke. 36:2725–2730. 2005. View Article : Google Scholar
34	Yi ZH, Fang YR and Yu SY: Research progress of gene differential expression of depression brain tissue. Zhongguo Shen Jing Jing Shen Ji Bing Za Zhi. 36:56–59. 2010.(In Chinese).
35	Ay I, Sugimori H and Finkelstein SP: Intravenous basic fibroblast growth factor (bFGF) decreases DNA fragmentation and prevents downregulation of Bcl-2 expression in the ischemic brain following middle cerebral artery occlusion in rats. Brain Res Mol Brain Res. 87:71–80. 2001. View Article : Google Scholar
36	Rosenblatt S, Irikura K, Caday CG, et al: Basic fibroblast growth factor dilates rat pial arterioles. J Cereb Blood Flow Metab. 14:70–74. 1994. View Article : Google Scholar : PubMed/NCBI
37	Longa EZ, Weinstein PR, Carlson S and Cummins R: Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke. 20:84–91. 1989. View Article : Google Scholar : PubMed/NCBI
38	Koizumi J, Yoshida Y, Nakazawa T and Ooneda G: Experimental studies of ischemic brain edema: A new experimental model of cerebral embolism in rats in which recirculation can be introduced in the ischemic area. Jpn J Stroke. 16:1–8. 1986.(In Japanese).
39	Willner P, Towell A, Sampson D, et al: Reduction of sucrose preference by chronic mild unpredictable stress, and its restoration by a tricyclic antidepressant. Psychopharmacology (Berl). 93:358–364. 1987. View Article : Google Scholar : PubMed/NCBI
40	Rasul A, El-Nour H, Blakely RD, et al: Effect of chronic mild stress on serotonergic markers in the skin and brain of the NC/Nga atopic-like mouse strain. Arch Dermatol Res. 303:625–633. 2011. View Article : Google Scholar : PubMed/NCBI
41	Ji YH and Song JG: Relevance among cognition function, autonomic nerve function and the serum levels of monoamine neurotransmitter in patients with post-stroke depression. Lin Chuang Shen Jing Bing Xue Za Zhi. 20:426–428. 2007.(In Chinese).
42	Loubinoux I, Kronenberg G, Endres M, et al: Post-stroke depression: mechanisms, translation and therapy. J Cell Mol Med. 16:1961–1969. 2012. View Article : Google Scholar : PubMed/NCBI
43	Haneda E, Higuchi M, Maeda J, et al: In vivo mapping of substance P receptors in brains of laboratory animals by high-resolution imaging systems. Synapse. 61:205–215. 2007. View Article : Google Scholar : PubMed/NCBI
44	Chatterjee K, Fall S and Barer D: Mood after stroke: a case control study of biochemical, neuro-imaging and socio-economic risk factors for major depression in stroke survivors. BMC Neurol. 10:1252010. View Article : Google Scholar : PubMed/NCBI
45	Evans SJ, Choudary PV, Neal CR, et al: Dysregulation of the fibroblast growth factor system in major depression. Proc Natl Acad Sci USA. 101:15506–15511. 2004. View Article : Google Scholar : PubMed/NCBI