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Article

Altered glutamate cysteine ligase activity in peripheral blood mononuclear cells from patients with systemic lupus erythematosus

  • Authors:
    • Weiqing Song
    • Jiangshui Yuan
    • Zongliang Zhang
    • Li Li
    • Lihua Hu
  • View Affiliations / Copyright

    Affiliations: Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China, Qingdao Municipal Hospital, Qingdao, Shandong 266071, P.R. China
  • Pages: 195-200
    |
    Published online on: April 24, 2014
       https://doi.org/10.3892/etm.2014.1689
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Abstract

Reductions in glutathione (GSH) levels have been shown to be associated with aging and the pathogenesis of a variety of diseases, including systemic lupus erythematosus (SLE). Glutamate cysteine ligase (GCL) catalyzes the first and rate‑limiting step of GSH synthesis. In order to appraise the correlation between oxidative stress and the severity and activity of SLE, GSH, oxidized GSH (GSSG) and thioredoxin (TRX) concentrations and the enzymatic activity levels of GCL in peripheral blood mononuclear cells (PBMCs) from patients with SLE and healthy controls were studied. In patients with SLE, the levels of GCL activity and GSH decreased, while TRX and GSSG levels increased when compared with those in the healthy controls. GSH concentrations and GCL activity levels negatively correlated with the SLE disease activity index and erythrocyte sedimentation rate. Furthermore, patients with SLE and nephritis had lower levels of GSH and GCL activity and higher levels of TRX and GSSG compared with those in SLE patients without nephritis. Therefore, the results of the present study indicate that insufficient levels of GSH and GCL activity in PBMCs may contribute to the pathogenesis of SLE.
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1 

Shah D, Aggarwal A, Bhatnagar A, et al: Association between T lymphocyte sub-sets apoptosis and peripheral blood mononuclear cells oxidative stress in systemic lupus erythematosus. Free Radic Res. 45:559–567. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Gordon C: Long-term complications of systemic lupus erythematosus. Rheumatology (Oxford). 41:1095–1100. 2002. View Article : Google Scholar : PubMed/NCBI

3 

Mansour RB, Lassoued S, Gargouri B, et al: Increased levels of autoantibodies against catalase and superoxide dismutase associated with oxidative stress in patients with rheumatoid arthritis and systemic lupus erythematosus. Scand J Rheumatol. 37:103–108. 2008. View Article : Google Scholar

4 

Kurien BT and Scofield RH: Free radical mediated peroxidative damage in systemic lupus erythematosus. Life Sci. 73:1655–1666. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Munoz LE, Gaipl US and Herrmann M: Predictive value of anti-dsDNA autoantibodies: importance of the assay. Autoimmun Rev. 7:594–597. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Li J, Ayene R, Ward KM, et al: Glucose deprivation increases nuclear DNA repair protein Ku and resistance to radiation induced oxidative stress in human cancer cells. Cell Biochem Funct. 27:93–101. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Townsend DM, Tew KD and Tapiero H: The importance of glutathione in human disease. Biomed Pharmacother. 57:145–155. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Gambhir JK, Lali P and Jain AK: Correlation between blood antioxidant levels and lipid peroxidation in rheumatoid arthritis. Clin Biochem. 30:351–355. 1997. View Article : Google Scholar : PubMed/NCBI

9 

Pastore A, Federici G, Bertini E and Piemonte F: Analysis of glutathione: implication in redox and detoxification. Clin Chim Acta. 333:19–39. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Burek CL and Rose NR: Autoimmune thyroiditis and ROS. Autoimmun Rev. 7:530–537. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Griffiths HR: Is the generation of neo-antigenic determinants by free radicals central to the development of autoimmune rheumatoid disease? Autoimmun Rev. 7:544–549. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Perricone C, De Carolis C and Perricone R: Glutathione: a key player in autoimmunity. Autoimmun Rev. 8:697–701. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Fernandez D and Perl A: Metabolic control of T cell activation and death in SLE. Autoimmun Rev. 8:184–189. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Dimitrov JD, Vassilev TL, Andre S, et al: Functional variability of antibodies upon oxidative processes. Autoimmun Rev. 7:574–578. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Crane FL and Low H: Reactive oxygen species generation at the plasma membrane for antibody control. Autoimmun Rev. 7:518–522. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Grisham MB: Reactive oxygen species in immune responses. Free Radic Biol Med. 36:1479–1480. 2004. View Article : Google Scholar : PubMed/NCBI

17 

Hassan SZ, Gheita TA, Kenawy SA, et al: Oxidative stress in systemic lupus erythematosus and rheumatoid arthritis patients: relationship to disease manifestations and activity. Int J Rheum Dis. 14:325–331. 2011.PubMed/NCBI

18 

Kurien BT and Scofield RH: Autoimmunity and oxidatively modified autoantigens. Autoimmun Rev. 7:567–573. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Huang CS, Chang LS, Anderson ME and Meister A: Catalytic and regulatory properties of the heavy subunit of rat kidney gamma-glutamylcysteine synthetase. J Biol Chem. 268:19675–19680. 1993.PubMed/NCBI

20 

McConnachie LA, Mohar I, Hudson FN, et al: Glutamate cysteine ligase modifier subunit deficiency and gender as determinants of acetaminophen-induced hepatotoxicity in mice. Toxicol Sci. 99:628–636. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Dalton TP, Dieter MZ, Yang Y, et al: Knockout of the mouse glutamate cysteine ligase catalytic subunit (Gclc) gene: Embryonic lethal when homozygous, and proposed model for moderate glutathione deficiency when heterozygous. Biochem Biophys Res Commun. 279:324–329. 2000. View Article : Google Scholar

22 

Kasuno K, Nakamura H, Ono T, et al: Protective roles of thioredoxin, a redox-regulating protein, in renal ischemia/reperfusion injury. Kidney Int. 64:1273–1282. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Maurice MM, Nakamura H, Gringhuis S, et al: Expression of the thioredoxin-thioredoxin reductase system in the inflamed joints of patients with rheumatoid arthritis. Arthritis Rheum. 42:2430–2439. 1999. View Article : Google Scholar : PubMed/NCBI

24 

Bombardier C, Gladman DD, Urowitz MB, et al: Derivation of the SLEDAI. A disease activity index for lupus patients The Committee on Prognosis Studies in SLE. Arthritis Rheum. 35:630–640. 1992. View Article : Google Scholar : PubMed/NCBI

25 

Hawker G, Gabriel S, Bombardier C, et al: A reliability study of SLEDAI: a disease activity index for systemic lupus erythematosus. J Rheumatol. 20:657–660. 1993.PubMed/NCBI

26 

Chen CN, Brown-Borg HM, Rakoczy SG, et al: Aging impairs the expression of the catalytic subunit of glutamate cysteine ligase in soleus muscle under stress. J Gerontol A Biol Sci Med Sci. 65:129–137. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Shah D, Sah S and Nath SK: Interaction between glutathione and apoptosis in systemic lupus erythematosus. Autoimmun Rev. 12:741–751. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Ortona E, Margutti P, Matarrese P, et al: Redox state, cell death and autoimmune diseases: a gender perspective. Autoimmun Rev. 7:579–584. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Ziegler DM: Role of reversible oxidation-reduction of enzyme thiols-disulfides in metabolic regulation. Annu Rev Biochem. 54:305–329. 1985. View Article : Google Scholar : PubMed/NCBI

30 

Maher P: The effects of stress and aging on glutathione metabolism. Ageing Res Rev. 4:288–314. 2005. View Article : Google Scholar : PubMed/NCBI

31 

Kasahara Y, Iwai K, Yachie A, et al: Involvement of reactive oxygen intermediates in spontaneous and CD95 (Fas/APO-1)-mediated apoptosis of neutrophils. Blood. 89:1748–1753. 1997.PubMed/NCBI

32 

Hammond CL, Madejczyk MS and Ballatori N: Activation of plasma membrane reduced glutathione transport in death receptor apoptosis of HepG2 cells. Toxicol Appl Pharmacol. 195:12–22. 2004. View Article : Google Scholar : PubMed/NCBI

33 

Messina JP and Lawrence DA: Cell cycle progression of glutathione-depleted human peripheral blood mononuclear cells is inhibited at S phase. J Immunol. 143:1974–1981. 1989.PubMed/NCBI

34 

Franco R, Panayiotidis MI and Cidlowski JA: Glutathione depletion is necessary for apoptosis in lymphoid cells independent of reactive oxygen species formation. J Biol Chem. 282:30452–30465. 2007. View Article : Google Scholar : PubMed/NCBI

35 

Ballatori N, Krance SM, Notenboom S, et al: Glutathione dysregulation and the etiology and progression of human diseases. Biol Chem. 390:191–214. 2009. View Article : Google Scholar : PubMed/NCBI

36 

Shah D, Kiran R, Wanchu A and Bhatnagar A: Oxidative stress in systemic lupus erythematosus: relationship to Th1 cytokine and disease activity. Immunol Lett. 129:7–12. 2010. View Article : Google Scholar : PubMed/NCBI

37 

Túri S, Németh I, Torkos A, et al: Oxidative stress and antioxidant defense mechanism in glomerular diseases. Free Radic Biol Med. 22:161–168. 1997.PubMed/NCBI

38 

Nakamura H, De Rosa SC, Yodoi J, et al: Chronic elevation of plasma thioredoxin: inhibition of chemotaxis and curtailment of life expectancy in AIDS. Proc Natl Acad Sci USA. 98:2688–2693. 2001. View Article : Google Scholar : PubMed/NCBI

39 

Jikimoto T, Nishikubo Y, Koshiba M, et al: Thioredoxin as a biomarker for oxidative stress in patients with rheumatoid arthritis. Mol Immunol. 38:765–772. 2002. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Song W, Yuan J, Zhang Z, Li L and Hu L: Altered glutamate cysteine ligase activity in peripheral blood mononuclear cells from patients with systemic lupus erythematosus. Exp Ther Med 8: 195-200, 2014.
APA
Song, W., Yuan, J., Zhang, Z., Li, L., & Hu, L. (2014). Altered glutamate cysteine ligase activity in peripheral blood mononuclear cells from patients with systemic lupus erythematosus. Experimental and Therapeutic Medicine, 8, 195-200. https://doi.org/10.3892/etm.2014.1689
MLA
Song, W., Yuan, J., Zhang, Z., Li, L., Hu, L."Altered glutamate cysteine ligase activity in peripheral blood mononuclear cells from patients with systemic lupus erythematosus". Experimental and Therapeutic Medicine 8.1 (2014): 195-200.
Chicago
Song, W., Yuan, J., Zhang, Z., Li, L., Hu, L."Altered glutamate cysteine ligase activity in peripheral blood mononuclear cells from patients with systemic lupus erythematosus". Experimental and Therapeutic Medicine 8, no. 1 (2014): 195-200. https://doi.org/10.3892/etm.2014.1689
Copy and paste a formatted citation
x
Spandidos Publications style
Song W, Yuan J, Zhang Z, Li L and Hu L: Altered glutamate cysteine ligase activity in peripheral blood mononuclear cells from patients with systemic lupus erythematosus. Exp Ther Med 8: 195-200, 2014.
APA
Song, W., Yuan, J., Zhang, Z., Li, L., & Hu, L. (2014). Altered glutamate cysteine ligase activity in peripheral blood mononuclear cells from patients with systemic lupus erythematosus. Experimental and Therapeutic Medicine, 8, 195-200. https://doi.org/10.3892/etm.2014.1689
MLA
Song, W., Yuan, J., Zhang, Z., Li, L., Hu, L."Altered glutamate cysteine ligase activity in peripheral blood mononuclear cells from patients with systemic lupus erythematosus". Experimental and Therapeutic Medicine 8.1 (2014): 195-200.
Chicago
Song, W., Yuan, J., Zhang, Z., Li, L., Hu, L."Altered glutamate cysteine ligase activity in peripheral blood mononuclear cells from patients with systemic lupus erythematosus". Experimental and Therapeutic Medicine 8, no. 1 (2014): 195-200. https://doi.org/10.3892/etm.2014.1689
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