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Article

Interferon‑α enhances the susceptibility of renal cell carcinoma to rapamycin by suppressing mTOR activity

  • Authors:
    • Xiao Han
    • Donghao Shang
    • Tiandong Han
    • Xiuhong Xu
    • Ye Tian
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China
  • Pages: 267-273
    |
    Published online on: April 25, 2014
       https://doi.org/10.3892/etm.2014.1691
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Abstract

The aim of the present study was to investigate the antiproliferative effects of interferon (IFN)‑α and rapamycin (RPM) on renal cell carcinoma (RCC) cells and examine the synergistic growth suppression conferred by IFN‑α and RPM. The effects of IFN‑α and/or RPM on RCC cells were determined using a WST‑1 assay and the synergy of IFN‑α and RPM against three RCC cell lines was analyzed with isobolographic analysis. The expression of mammalian target of rapamycin (mTOR) was downregulated by RNAi, and the expression and phosphorylation of proteins in the mTOR pathway following treatment with IFN‑α and/or RPM was examined by western blot analysis. The observations indicated that IFN‑α significantly increased the susceptibility of RCC cells to RPM and the synergistic effect of IFN‑α and RPM against RCC cells was confirmed in all three RCC cell lines. The mTOR pathway was shown to be associated with the synergistic effect of IFN‑α and RPM against RCC. IFN‑α and RPM alone decreased the phosphorylation of mTOR, p70 S6 kinase, S6 and 4E binding protein 1, and IFN‑α significantly enhanced the RPM‑induced suppression of the mTOR pathway. However, in RCC cells with low mTOR activity, the synergy of IFN‑α and RPM was eliminated. Therefore, the results of the present study indicate that the mTOR pathway plays an important role in the synergistic effect of IFN‑α and RPM against RCC cells. Thus, mTOR may serve as an effective therapeutic target in the treatment of advanced RCC.
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Copy and paste a formatted citation
Spandidos Publications style
Han X, Shang D, Han T, Xu X and Tian Y: Interferon‑α enhances the susceptibility of renal cell carcinoma to rapamycin by suppressing mTOR activity. Exp Ther Med 8: 267-273, 2014.
APA
Han, X., Shang, D., Han, T., Xu, X., & Tian, Y. (2014). Interferon‑α enhances the susceptibility of renal cell carcinoma to rapamycin by suppressing mTOR activity. Experimental and Therapeutic Medicine, 8, 267-273. https://doi.org/10.3892/etm.2014.1691
MLA
Han, X., Shang, D., Han, T., Xu, X., Tian, Y."Interferon‑α enhances the susceptibility of renal cell carcinoma to rapamycin by suppressing mTOR activity". Experimental and Therapeutic Medicine 8.1 (2014): 267-273.
Chicago
Han, X., Shang, D., Han, T., Xu, X., Tian, Y."Interferon‑α enhances the susceptibility of renal cell carcinoma to rapamycin by suppressing mTOR activity". Experimental and Therapeutic Medicine 8, no. 1 (2014): 267-273. https://doi.org/10.3892/etm.2014.1691
Copy and paste a formatted citation
x
Spandidos Publications style
Han X, Shang D, Han T, Xu X and Tian Y: Interferon‑α enhances the susceptibility of renal cell carcinoma to rapamycin by suppressing mTOR activity. Exp Ther Med 8: 267-273, 2014.
APA
Han, X., Shang, D., Han, T., Xu, X., & Tian, Y. (2014). Interferon‑α enhances the susceptibility of renal cell carcinoma to rapamycin by suppressing mTOR activity. Experimental and Therapeutic Medicine, 8, 267-273. https://doi.org/10.3892/etm.2014.1691
MLA
Han, X., Shang, D., Han, T., Xu, X., Tian, Y."Interferon‑α enhances the susceptibility of renal cell carcinoma to rapamycin by suppressing mTOR activity". Experimental and Therapeutic Medicine 8.1 (2014): 267-273.
Chicago
Han, X., Shang, D., Han, T., Xu, X., Tian, Y."Interferon‑α enhances the susceptibility of renal cell carcinoma to rapamycin by suppressing mTOR activity". Experimental and Therapeutic Medicine 8, no. 1 (2014): 267-273. https://doi.org/10.3892/etm.2014.1691
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