Direct identification of Mycobacterium abscessus through 16S rDNA sequence analysis and a citrate utilization test: A case report

Zou,Ziying; Liu,Yuan; Zhu,Bing; Zeng,Ping

doi:10.3892/etm.2014.1705

July-2014 Volume 8 Issue 1

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July-2014 Volume 8 Issue 1

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Case Report

Direct identification of Mycobacterium abscessus through 16S rDNA sequence analysis and a citrate utilization test: A case report

Authors:
- Ziying Zou
- Yuan Liu
- Bing Zhu
- Ping Zeng
View Affiliations / Copyright

Affiliations: Department of Microbiology and Immunology, Center of Laboratory Medicine, General Hospital of Chengdu Military Region of PLA, Chengdu 610083, P.R. China, Department of Clinical Chemistry, Center of Laboratory Medicine, General Hospital of Chengdu Military Region of PLA, Chengdu 610083, P.R. China
Pages: 115-117
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Published online on: May 12, 2014

https://doi.org/10.3892/etm.2014.1705
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Abstract

A growing number of nontuberculous mycobacteria infection cases, especially those caused by rapidly growing mycobacteria (RGM), have been reported in the past decade. Conventional methods for mycobacteria diagnosis are inefficient and easily lead to misdiagnosis. New detection methods, such as gene sequencing, have been reported but are not widely used. The aim of the present case report was to provide a quick and exact method of identifying Myobacterium abscessus (M. abscessus) infections. The particular case reported in this study initially manifested as hyperglycemia and papules in the right leg. Routine cultures for fungus were repeatedly negative. However, cultures of the purulent material under aerobic cultivation for five days yielded a rapidly growing, nontuberculous mycobacterium. A Ziehl-Neelsen staining of this mycobacterium revealed the presence of acid-fast bacilli that were finally identified as M. abscessus through 16S rDNA sequence analysis and a citrate utilization test. The current report may help other clinicians to make a quick and accurate diagnosis of RGM infection.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Direct identification of Mycobacterium abscessus through 16S rDNA sequence analysis and a citrate utilization test: A case report

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Abstract

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