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Article

In vivo determination of muscle‑derived stem cells in the rat corpus cavernosum

  • Authors:
    • Lijun Xu
    • Boxin Xue
    • Yuxi Shan
    • Dong Chen
    • Jie Gao
    • Dongrong Yang
    • Chuanyang Sun
    • Yong Cui
  • View Affiliations / Copyright

    Affiliations: Department of Urinary Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China
  • Pages: 274-280
    |
    Published online on: May 14, 2014
       https://doi.org/10.3892/etm.2014.1710
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Abstract

The aim of the present in vivo study was to determine the presence of muscle‑derived stem cells (MDSCs) in the corpus cavernosum of rats. Immunohistochemistry and reverse transcription polymerase chain reaction (RT‑PCR) were performed to detect the expression of the stem cell markers stem cell antigen-1 (Sca‑1), Oct4 and Desmin in Sprague‑Dawley rats aged 2, 5 and 20 months. Sca‑1 was mainly expressed in the blood vessels and cavernous sinus and staining revealed that Sca‑1 was predominantly expressed in the cytoplasm. Desmin was primarily expressed in muscular tissues and staining demonstrated that it was mainly expressed in the cytoplasm, however, Desmin was also partially expressed in the nuclei. A small number of double positive cells, expressing Sca‑1 and Desmin, were also detected near the cavernous sinus. It was found that the expression of the markers was negatively correlated with the age of the rats (P<0.05). The results from the RT‑PCR demonstrated that the expression levels of Sca‑1 and Desmin significantly decreased with age (P<0.05). In addition, the correlation analysis indicated that the expression of Sca‑1 and Desmin were negatively correlated with the age of the rats (r=‑0.929; P<0.05). In conclusion, the present study provided evidence for the presence of MDSCs in the rat corpus cavernosum. MDSCs may be a potential therapeutic treatment for organic erectile dysfunction.
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Copy and paste a formatted citation
Spandidos Publications style
Xu L, Xue B, Shan Y, Chen D, Gao J, Yang D, Sun C and Cui Y: In vivo determination of muscle‑derived stem cells in the rat corpus cavernosum. Exp Ther Med 8: 274-280, 2014.
APA
Xu, L., Xue, B., Shan, Y., Chen, D., Gao, J., Yang, D. ... Cui, Y. (2014). In vivo determination of muscle‑derived stem cells in the rat corpus cavernosum. Experimental and Therapeutic Medicine, 8, 274-280. https://doi.org/10.3892/etm.2014.1710
MLA
Xu, L., Xue, B., Shan, Y., Chen, D., Gao, J., Yang, D., Sun, C., Cui, Y."In vivo determination of muscle‑derived stem cells in the rat corpus cavernosum". Experimental and Therapeutic Medicine 8.1 (2014): 274-280.
Chicago
Xu, L., Xue, B., Shan, Y., Chen, D., Gao, J., Yang, D., Sun, C., Cui, Y."In vivo determination of muscle‑derived stem cells in the rat corpus cavernosum". Experimental and Therapeutic Medicine 8, no. 1 (2014): 274-280. https://doi.org/10.3892/etm.2014.1710
Copy and paste a formatted citation
x
Spandidos Publications style
Xu L, Xue B, Shan Y, Chen D, Gao J, Yang D, Sun C and Cui Y: In vivo determination of muscle‑derived stem cells in the rat corpus cavernosum. Exp Ther Med 8: 274-280, 2014.
APA
Xu, L., Xue, B., Shan, Y., Chen, D., Gao, J., Yang, D. ... Cui, Y. (2014). In vivo determination of muscle‑derived stem cells in the rat corpus cavernosum. Experimental and Therapeutic Medicine, 8, 274-280. https://doi.org/10.3892/etm.2014.1710
MLA
Xu, L., Xue, B., Shan, Y., Chen, D., Gao, J., Yang, D., Sun, C., Cui, Y."In vivo determination of muscle‑derived stem cells in the rat corpus cavernosum". Experimental and Therapeutic Medicine 8.1 (2014): 274-280.
Chicago
Xu, L., Xue, B., Shan, Y., Chen, D., Gao, J., Yang, D., Sun, C., Cui, Y."In vivo determination of muscle‑derived stem cells in the rat corpus cavernosum". Experimental and Therapeutic Medicine 8, no. 1 (2014): 274-280. https://doi.org/10.3892/etm.2014.1710
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