Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
July-2014 Volume 8 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2014 Volume 8 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Arsenic trioxide induces apoptosis in the THP1 cell line by downregulating EVI‑1

  • Authors:
    • Ling‑Yun Zhou
    • Fang‑Yuan Chen
    • Li‑Jing Shen
    • Hai‑Xia Wan
    • Ji‑Hua Zhong
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. China
  • Pages: 85-90
    |
    Published online on: May 15, 2014
       https://doi.org/10.3892/etm.2014.1716
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Acute leukemia is a malignant clonal hematopoietic stem cell disease. In the current study, the effects of arsenic trioxide (ATO) on the ecotropic viral integration site‑1 (EVI‑1) gene were investigated in the THP1 cell line. THP-1 cells were treated with different concentrations of ATO (0, 1, 3 and 5 µM) for 24, 48 or 72 h, then tested for cell viability by CCK-8 kit, cell morphology by cytospin smear, cell apoptosis by flow cytometry, EVI-1 mRNA expression by reverse transcription polymerase chain reaction (RT-PCR) and protein quantity by western blot. ATO treatment was shown to inhibit proliferation and induce apoptosis in THP1 cells in a dose‑ and time‑dependent manner. ATO downregulated the mRNA and protein expression of EVI‑1 in the THP1 cell line. In addition, ATO significantly decreased the expression of antiapoptotic proteins, B‑cell lymphoma 2 (Bcl‑2) and B cell lymphoma‑extra large (Bcl‑xL), but markedly increased the expression of proapoptotic proteins, including c‑Jun N‑terminal kinase (JNK), phosphorylated‑JNK, Bax, full length caspase‑3 and cleaved caspase‑3. These results indicated that ATO inhibited the proliferation and induced apoptosis in THP1 cells partially via blocking the inhibitory effects of EVI‑1 on the JNK signaling pathway with the involvement of apoptosis‑associated proteins, including Bax, Bcl‑2, Bcl‑xL and caspase‑3. These novel observations may be used to elucidate the mechanism by which ATO induces apoptosis in acute leukemia cells, and provide rationales to develop a personalized medicine strategy for ATO via targeting EVI‑1 positive neoplasm.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Liang R, Bai QX, Zhang YQ, et al: Reduced tumor lysis syndrome with low dose chemotherapy for hyperleukocytic acute leukemia prior to induction therapy. Asian Pac J Cancer Prev. 12:1807–1811. 2011.PubMed/NCBI

2 

Krivtsov AV, Figueroa ME, Sinha AU, et al: Cell of origin determines clinically relevant subtypes of MLL-rearranged AML. Leukemia. 27:852–860. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Barjesteh van Waalwijk van Doorn-Khosrovani S, Erpelinck C, van Putten WL, et al: High EVI1 expression predicts poor survival in acute myeloid leukemia: a study of 319 de novo AML patients. Blood. 101:837–845. 2003.PubMed/NCBI

4 

Konantz M, André MC, Ebinger M, et al: EVI-1 modulates leukemogenic potential and apoptosis sensitivity in human acute lymphoblastic leukemia. Leukemia. 27:56–65. 2013. View Article : Google Scholar : PubMed/NCBI

5 

De Weer A, Poppe B, Cauwelier B, et al: Screening for EVI1: ectopic expression absent in T-cell acute lymphoblastic leukemia patients and cell lines. Cancer Genet Cytogenet. 171:79–80. 2006.PubMed/NCBI

6 

Valk PJ, Verhaak RG, Beijen MA, et al: Prognostically useful gene-expression profiles in acute myeloid leukemia. N Engl J Med. 350:1617–1628. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Yuasa H, Oike Y, Iwama A, et al: Oncogenic transcription factor Evi1 regulates hematopoietic stem cell proliferation through GATA-2 expression. EMBO J. 24:1976–1987. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Goyama S, Yamamoto G, Shimabe M, et al: Evi-1 is a critical regulator for hematopoietic stem cells and transformed leukemic cells. Cell Stem Cell. 3:207–220. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Hoyt PR, Bartholomew C, Davis AJ, et al: The Evi1 proto-oncogene is required at midgestation for neural, heart, and paraxial mesenchyme development. Mech Dev. 65:55–70. 1997. View Article : Google Scholar : PubMed/NCBI

10 

Brooks DJ, Woodward S, Thompson FH, et al: Expression of the zinc finger gene EVI-1 in ovarian and other cancers. Br J Cancer. 74:1518–1525. 1996. View Article : Google Scholar : PubMed/NCBI

11 

Morishita K, Parganas E, Douglass EC and Ihle JN: Unique expression of the human Evi-1 gene in an endometrial carcinoma cell line: sequence of cDNAs and structure of alternatively spliced transcripts. Oncogene. 5:963–971. 1990.PubMed/NCBI

12 

Fears S, Mathieu C, Zeleznik-Le N, Huang S, Rowley JD and Nucifora G: Intergenic splicing of MDS1 and EVI1 occurs in normal tissues as well as in myeloid leukemia and produces a new member of the PR domain family. Proc Natl Acad Sci USA. 93:1642–1647. 1996. View Article : Google Scholar : PubMed/NCBI

13 

Jiang GL and Huang S: The yin-yang of PR-domain family genes in tumorigenesis. Histol Histopathol. 15:109–117. 2000.PubMed/NCBI

14 

Nitta E, Izutsu K, Yamaguchi Y, et al: Oligomerization of Evi-1 regulated by the PR domain contributes to recruitment of corepressor CtBP. Oncogene. 24:6165–6173. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Shackelford D, Kenific C, Blusztajn A, Waxman S and Ren R: Targeted degradation of the AML1/MDS1/EVI1 oncoprotein by arsenic trioxide. Cancer Res. 66:11360–11369. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Dhanasekaran DN and Reddy EP: JNK signaling in apoptosis. Oncogene. 27:6245–6251. 2008. View Article : Google Scholar

17 

Kurokawa M, Mitani K, Yamagata T, et al: The evi-1 oncoprotein inhibits c-Jun N-terminal kinase and prevents stress-induced cell death. EMBO J. 19:2958–2968. 2000. View Article : Google Scholar : PubMed/NCBI

18 

Garrison SP, Phillips DC, Jeffers JR, et al: Genetically defining the mechanism of Puma- and Bim-induced apoptosis. Cell Death Differ. 19:642–649. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Coiras M, López-Huertas MR, Mateos E and Alcami J: Caspase-3-mediated cleavage of p65/RelA results in a carboxy-terminal fragment that inhibits IkappaBalpha and enhances HIV-1 replication in human T lymphocytes. Retrovirology. 5:1092008. View Article : Google Scholar : PubMed/NCBI

20 

Sun C, Guo XX, Zhu D, et al: Apoptosis is induced in cancer cells via the mitochondrial pathway by the novel xylocydine-derived compound JRS-15. Int J Mol Sci. 14:850–870. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Yang Y, Duan W, Liang Z, et al: Curcumin attenuates endothelial cell oxidative stress injury through Notch signaling inhibition. Cell Signal. 25:615–629. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Lin WJ, Shen LJ, Chen FY, Zhong M, Sun RL and Zhong JH: The establishment of AML1-EVI1 transgenic zebrafish model and regulation of hematopoietic transcription factors. Journal of Internal Medicine Concepts & Practice. 3:192–196. 2013.(In Chinese).

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhou LY, Chen FY, Shen LJ, Wan HX and Zhong JH: Arsenic trioxide induces apoptosis in the THP1 cell line by downregulating EVI‑1. Exp Ther Med 8: 85-90, 2014.
APA
Zhou, L., Chen, F., Shen, L., Wan, H., & Zhong, J. (2014). Arsenic trioxide induces apoptosis in the THP1 cell line by downregulating EVI‑1. Experimental and Therapeutic Medicine, 8, 85-90. https://doi.org/10.3892/etm.2014.1716
MLA
Zhou, L., Chen, F., Shen, L., Wan, H., Zhong, J."Arsenic trioxide induces apoptosis in the THP1 cell line by downregulating EVI‑1". Experimental and Therapeutic Medicine 8.1 (2014): 85-90.
Chicago
Zhou, L., Chen, F., Shen, L., Wan, H., Zhong, J."Arsenic trioxide induces apoptosis in the THP1 cell line by downregulating EVI‑1". Experimental and Therapeutic Medicine 8, no. 1 (2014): 85-90. https://doi.org/10.3892/etm.2014.1716
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou LY, Chen FY, Shen LJ, Wan HX and Zhong JH: Arsenic trioxide induces apoptosis in the THP1 cell line by downregulating EVI‑1. Exp Ther Med 8: 85-90, 2014.
APA
Zhou, L., Chen, F., Shen, L., Wan, H., & Zhong, J. (2014). Arsenic trioxide induces apoptosis in the THP1 cell line by downregulating EVI‑1. Experimental and Therapeutic Medicine, 8, 85-90. https://doi.org/10.3892/etm.2014.1716
MLA
Zhou, L., Chen, F., Shen, L., Wan, H., Zhong, J."Arsenic trioxide induces apoptosis in the THP1 cell line by downregulating EVI‑1". Experimental and Therapeutic Medicine 8.1 (2014): 85-90.
Chicago
Zhou, L., Chen, F., Shen, L., Wan, H., Zhong, J."Arsenic trioxide induces apoptosis in the THP1 cell line by downregulating EVI‑1". Experimental and Therapeutic Medicine 8, no. 1 (2014): 85-90. https://doi.org/10.3892/etm.2014.1716
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team